Efecto de la dosificación de CO2 en la cinética de crecimiento de microalgas Chlorella vulgaris y Scenedesmus obliquuss

Milton Cesar Ararat Orozco; Oscar Eduardo Sanclemente Reyes; Leonardo Vergara Patiño

doi:10.22490/21456453.3482

Vol. 12 No. 1 (2021)

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2020-12-23

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Ararat Orozco, M. C., Sanclemente Reyes, O. E., & Vergara Patiño, L. (2020). Effect of CO2 dosing on growth kinetics of Chlorella vulgaris and Scenedesmus obliquus microalgae. Revista De Investigación Agraria Y Ambiental, 12(1), 89-100. https://doi.org/10.22490/21456453.3482

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Effect of CO2 dosing on growth kinetics of Chlorella vulgaris and Scenedesmus obliquus microalgae

DOI: https://doi.org/10.22490/21456453.3482

Section

Área Ambiental

Milton Cesar Ararat Orozco Universidad Nacional Abierta y a Distancia (UNAD)

Oscar Eduardo Sanclemente Reyes Universidad Nacional Abierta y a Distancia (UNAD)

Leonardo Vergara Patiño Corporación Autónoma Regional del Valle del Cauca- CVC.

Contextualization: Currently, interest in the production of microalgae has increased due to the different uses of microalgal biomass in some industrial sectors, in addition to being used in the mitigation of greenhouse gases thanks to its high capacity for CO₂ capture.

Knowledge gap: Being part of the Paris Agreement in 2015, Colombia is committed to reduce its greenhouse gases emissions by 20%, to contribute to the goal set for 2050, for this reason, it must adopt research and action plans focused on this objective.

Purpose: the purpose of this study was to evaluate the effect of CO₂ induction at different concentrations on the growth kinetics of Chlorella Vulgaris CV_2714A and Scenedesmus Obliquus SOB_001 in a laboratory scale photobioreactor system in a volume of 3.8 L of culture medium (NPK solution + micronutrients).

Methodology: The tests were carried out in photoperiods with 12 hours of light and 12 hours of darkness for 5 consecutive days. The completely randomized experimental design whose sources of variation were microalgae species and CO₂ dosage (0.2, 6.5 and 12.8 L. day^-1) with artificial lighting by a 4400-lumen fluorescent lamp. The cellular concentration was estimated by means of the numerical approximation in the “Neubauer” chamber with the methodology used by Darki et al. (2017), in addition to growth rate and cell doubling time in days according to Andersen, (2005). The data were submitted to analysis of variance and Tukey's mean tests (p<0.05).

Results and conclusions: The results in the different tests showed a higher cell concentration in C. vulgaris compared to S. obliquus. Regarding CO₂ supply, the 2 species obtained a significantly greater response (p<0.05) at the dose of 6.5 L. day^-1, reaching a cellular concentration of 2.59 x 10⁷ cel.ml^-1 and 4.62 x 10⁶ cel.ml^-1, respectively. These results allow us to conclude that the cultivation of these microalgae associated with the dose of CO₂ can favor their rapid growth and biomass production, while also contributing to other studies on the reduction of greenhouse gases.

Climate change, photobioreactors, cell growth, biomass

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Effect of CO2 dosing on growth kinetics of Chlorella vulgaris and Scenedesmus obliquus microalgae

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