Copyright (c) 2026 Publicaciones e Investigación
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
When the Publicaciones e Investigaciones Journal receives an original study or article from its author(s), whether by email, postal service, or the platforms available for said purpose, know that it may be published in physical or electronic formats in national or international archives, databases, or SIRES. As such, Publications and Research authorizes the reproduction and citation of said material, provided that the description of information is carried out in conformity with bibliographic norms, and mention the corresponding names, authors, article, issue, and pages. Publications and Research, in advance, expresses that the information, concepts, and methods are the responsibility of the author(s). As such, the UNAD does not have any influence whatsoever over that expressed in the manuscript.
Production and purification of exopolysaccharide (EPS) under liquid fermentation using Bacillus megaterium ATCC 14581
This study evaluated the ability of Bacillus megaterium ATCC 14581 to produce and purify exopolysaccharides (EPS) under controlled liquid fermentation conditions. Glucose and sucrose (5 g/L) were used as carbon sources to compare their efficiency. Results indicated that sucrose favored greater colony development (4.8 mm) and the highest final mass of purified EPS (2.691 g). However, glucose demonstrated higher efficiency in biomass yield (Yx/s=0.498 g/g) and product yield (Yps= 1.059 g/g) compared to 0.378 g/g and 0.968 g/g obtained with sucrose, respectively. Qualitative tests, such as the Ropy test and mobility assays (swimming and swarming), confirmed the synthesis of extracellular matrices and the microorganism's adaptation capacity. The purification process, conducted via cold ethanol precipitation, was validated through spectrophotometric methods (Dubois and DNS), confirming the polysaccharide nature of the product and the absence of residual reducing sugars. It is concluded that B. megaterium holds high biotechnological potential for EPS production, with sucrose being the most effective source for increasing the production of this polymer.
How to Cite
