Adaptación del Método Bligh & Dyer a la Extracción de Lípidos de Microalgas Colombianas para la Producción de Biodiesel de Tercera Generación

González Delgado Ángel; Galindo Leisslly; González Silvia; Peralta Ruiz Yeimmy; Kafarov Viatchesla

doi:10.22490/25394088.1093

Vol. 6 (2012)

Published

2012-06-02

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Adaptation Method Bligh & Dyer a Lipid Extraction of Colomb ian Microalgas Biodiesel Production for Third Generation

DOI: https://doi.org/10.22490/25394088.1093

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González Delgado Ángel Escuela de Ciencia Agrícolas y Pecuarias, Universidad Nacional Abierta y a Distancia-UNAD

Galindo Leisslly Universidad Industrial de Santander-UIS.

González Silvia Universidad Nacional Abierta y a Distancia-UNAD.

Peralta Ruiz Yeimmy Escuela de Ciencias Básicas Tecnología e Ingeniería, Universidad Nacional Abierta y a Distancia-UNAD.

Kafarov Viatchesla Centro de Investigación para el Desarrollo Sostenible en Industria y Energía, Universidad Industrial de Santander.

In the biodiesel production process from microalgae, the cell disruption and lipid extraction stages are important for obtaining triglycerides that can be transesterified to biodiesel and glycerol. In this work, the Bligh & Dyer method was adapted for lipid extraction from native microalgae using organosolv pretreatment or acid hydrolysis as cell disruption mechanism for improve the extraction process. Chloroform-methanol-water are the solvents employed in the Bligh & Dyer extraction method. The microalgae species Botryococcus braunii, Nannocloropsis, Closterium, Guinardia y Amphiprora were employed for the experimental part. Adaptation of the method was found the best extraction conditions, these were: 1:20 biomass/solvent ratio, initial ratio solvents CHCl3:CH3OH:H2O (1:2:0), stirring conditions of 5000 rpm for 14 minutes and centrifuge of 3400 rpm for 15 minutes. The cell disruption mechanisms allowed to obtain extracts with high lipid content after performing the extraction with Bligh & Dyer method, but decreases significantly the total extraction yield. Finally, the fatty acids profiles showed that Botryococcus braunii specie contains higher acylglycerol percentage area suitable for the production of biodiesel.

biomass, bligh & dyer method, cell disruption, extraction, lipids, microalgae

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Delgado Ángel, G., Leisslly, G., Silvia, G., Ruiz Yeimmy, P., & Viatchesla, K. (2012). Adaptation Method Bligh & Dyer a Lipid Extraction of Colomb ian Microalgas Biodiesel Production for Third Generation. Publicaciones E Investigación, 6, 25-34. https://doi.org/10.22490/25394088.1093

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Adaptation Method Bligh & Dyer a Lipid Extraction of Colomb ian Microalgas Biodiesel Production for Third Generation

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