Adaptación del Método Bligh & Dyer a la Extracción de Lípidos de Microalgas Colombianas para la Producción de Biodiesel de Tercera Generación

González Delgado Ángel; Galindo Leisslly; González Silvia; Peralta Ruiz Yeimmy; Kafarov Viatchesla

doi:10.22490/25394088.1093

Vol. 6 (2012)

Published 2012-06-02

license

When the Publicaciones e Investigaciones Journal receives an original study or article from its author(s), whether by email, postal service, or the platforms available for said purpose, know that it may be published in physical or electronic formats in national or international archives, databases, or SIRES. As such, Publications and Research authorizes the reproduction and citation of said material, provided that the description of information is carried out in conformity with bibliographic norms, and mention the corresponding names, authors, article, issue, and pages. Publications and Research, in advance, expresses that the information, concepts, and methods are the responsibility of the author(s). As such, the UNAD does not have any influence whatsoever over that expressed in the manuscript.

Original article

Adaptation Method Bligh & Dyer a Lipid Extraction of Colomb ian Microalgas Biodiesel Production for Third Generation

DOI: https://doi.org/10.22490/25394088.1093

González Delgado Ángel Universidad Nacional Abierta y a Distancia

Galindo Leisslly Universidad Industrial de Santander-UIS.

González Silvia Universidad Nacional Abierta y a Distancia

Peralta Ruiz Yeimmy Universidad Nacional Abierta y a Distancia

Kafarov Viatchesla Centro de Investigación para el Desarrollo Sostenible en Industria y Energía, Universidad Industrial de Santander.

PDF (Spanish)

Abstract
References

In the biodiesel production process from microalgae, the cell disruption and lipid extraction stages are important for obtaining triglycerides that can be transesterified to biodiesel and glycerol. In this work, the Bligh & Dyer method was adapted for lipid extraction from native microalgae using organosolv pretreatment or acid hydrolysis as cell disruption mechanism for improve the extraction process. Chloroform-methanol-water are the solvents employed in the Bligh & Dyer extraction method. The microalgae species Botryococcus braunii, Nannocloropsis, Closterium, Guinardia y Amphiprora were employed for the experimental part. Adaptation of the method was found the best extraction conditions, these were: 1:20 biomass/solvent ratio, initial ratio solvents CHCl3:CH3OH:H2O (1:2:0), stirring conditions of 5000 rpm for 14 minutes and centrifuge of 3400 rpm for 15 minutes. The cell disruption mechanisms allowed to obtain extracts with high lipid content after performing the extraction with Bligh & Dyer method, but decreases significantly the total extraction yield. Finally, the fatty acids profiles showed that Botryococcus braunii specie contains higher acylglycerol percentage area suitable for the production of biodiesel.

keywords: biomass, bligh & dyer method, cell disruption, extraction, lipids, microalgae

A. Demirbas, “Biofuels sources, biofuel policy, biofuel economy and global biofuel projections”. Energy Conversion and Management, Vol. 49, pp. 2106- 2116., 2008.

S. Majer, F. Mueller-Langer, V. Zeller and M. Kaltschmitt. “Implications of biodiesel production and utilization on global climate a literature review”. European Journal of Lipid Science Technology, vol. 111, No. 8, pp.747-762, 2009.

P. Schenk, S. Thomas-Hall, E. Stephen, U. Marx, J. Mussgnug, C. Posten, O. Kruse, and B. Hankamer. “Second generation biofuels: high-efficiency microalgae for biodiesel production”. Bioenergy Research, vol. 1, No. 1, pp. 20-43, 2008.

N. Singh and A. Singh. “Production of lipids biofuels from renewable resources”. Progress in energy and combustion science, pp. 1-17, 2010.

J. Rosenberg, G. Oylerl, L. Wilkinson and M. Betenbaugh. “A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution”. Current Opinion in Biotechnology, vol. 19, pp. 430-436, 2008.

Chisti Y. “Biodiesel from microalgae”. Biotechnology Advances, vol. 25, pp. 294–306, 2007.

E. Bligh and W. Dyer. “A rapid method of total lipid extraction and purification”. Journal Biochemical Physiology, vol. 37, No. 8, pp. 911-917, 1959.

M. Jaramillo and N. Mantilla. Estudio del método de pretratamiento químico etanol-NaOH para la obtención de azúcares reductores a partir de microalgas, como vía a la producción de bioetanol. Universidad Industrial de Santander, Bucaramanga, 25 p., 2010.

A. Dominguez J. Fábregas and A. Otero. “Astaxantina, el oro rojo de la microalga” Haematococcus Pluvialis. Algas: Boletín Española de ficología: pp. 160-167, 2006.

G. Cravotto, L Boffa, S. Mantegna, P. Perego, M. Avogadro and P. Cintas 2. “Improved extraction of vegetable oils under high-intensity ultrasound and/or microwaves”. Ultrasonics sonochemistry, vol. 15, No. 5, pp. 898-902, 2008.

S., Lee B. Yoon, and H. Oh 1. “Rapid method for the determination of lipid from the green alga Botryococcus braunii.” Biotechnology Techniques, vol. 12, No. 7, pp. 553- 556, 1998.

Determinación de grasa método Bligh and Dyer. Instituto de salud pública de Chile. Subdepartamento laboratorios del ambiente. Sección química de alimentos. PRT: 701. 04-156.

license

How to Cite

Delgado Ángel, G., Leisslly, G., Silvia, G., Ruiz Yeimmy, P., & Viatchesla, K. (2012). Adaptation Method Bligh & Dyer a Lipid Extraction of Colomb ian Microalgas Biodiesel Production for Third Generation. Publicaciones E Investigación, 6, 25-34. https://doi.org/10.22490/25394088.1093

Download Citation

Almétricas

Metrics

Metrics Loading ...