Germinación conidial y crecimiento micelial in vitro de Trichoderma koningiopsis (Th003) con agroquímicos de papa

Blanca Lucía Botina Azaín; Camilo Rubén Beltrán Acosta; Yimmy Alexander Zapata Narvaéz

doi:10.22490/21456453.9186

Vol. 17 No. 1 (2026)

Published 2025-12-02

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Área Agrícola

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Conidial germination and in vitro mycelial growth of Trichoderma koningiopsis (Th003) with potato agrochemicals

DOI: https://doi.org/10.22490/21456453.9186

Blanca Lucía Botina Azaín AGROSAVIA

Camilo Rubén Beltrán Acosta AGROSAVIA

Yimmy Alexander Zapata Narvaéz AGROSAVIA

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Abstract
References

Contextualization: Different pests affect potato crop production and given the trends towards sustainable agricultural production; it is important to implement management alternatives that allow to reduce the use of chemical agro-inputs.

Knowledge gap: The negative impact that the different agrochemicals used in potato cultivation may have on the growth of Trichoderma koningiopsis Th003, the active ingredient of the biopesticide Tricotec® WG, is unknown.

Purpose: To evaluate in vitro the effect of agrochemicals used in potato cultivation on mycelial growth and germination of conidia of T. koningiopsis Th003, with a view to establishing recommendations for the use of the biopesticide.

Methodology: The effect on mycelial growth was evaluated on PDA agar supplemented with each i.a. at the higher dose recommended in the data sheet. The effect on conidia germination was performed by suspending them in solutions of each agrochemical for one hour and then seeding on water agar.

Results and conclusions: Insecticides such as Acephate, Bifenthrin, Chlorantraniliprole, Chlorfenapyr, Fipronil, Methomyl, Phenthoate, Lambdacyhalothrin-Tiamethoxam, and Thiamethoxam-Chlorantraniliprole, the bactericide based oxolinic acid and the fungicide N-Alkyl Benzyl and all fertilizers (except Boron Ethanolamine, inhibiting mycelial growth by 22% and conidial germination by 34%) did not inhibit the growth of T. koningiopsis Th003. The bactericides based on gentamicin sulfate and oxytetracycline can be considered moderately compatible given their relative inhibitory effect on germination, as well as the fungicide Thiophanate-methyl. The fungicides Pyraclostrobin-Fluxapyroxad and Azoxystrobin-Mancozeb, the disinfectants Benzalkonium chloride+surfactants and the Iodine complex inhibited the growth and germination of fungal conidia. Potassium Silicate and Ethoxylated Alcohol-Polyoxyethylene Alkyl Ether adjuvants did not inhibit germination.

keywords: adjuvant, fertilizer, germination, growth, pesticide

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How to Cite

Botina Azaín, B. L., Beltrán Acosta, C. R., & Zapata Narvaéz, Y. A. (2025). Conidial germination and in vitro mycelial growth of Trichoderma koningiopsis (Th003) with potato agrochemicals. Revista De Investigación Agraria Y Ambiental, 17(1), 55-72. https://doi.org/10.22490/21456453.9186

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