Evaluation of culture media for growth of probiotic lactic acid bacteria and their potential use in poultry feeding
In Colombia, the poultry industry has shown a constant growth in recent years, however, in line with this increase, the use of antibiotics to maintain animal health has also been increasing, causing the appearance of resistant strains such as Escherichia coli, Salmonella and Campylobacter, generating a public health problem. Multiple studies have shown that the use of probiotic bacteria (LAB) with antimicrobial capacity can be used in animal feed, reducing the use of antibiotics. For the industrial use of LAB, it is necessary to find economical and adequate culture media for the recovery and growth of the strains. For this reason, during the present study, the adequate concentration of the substrate soy milk, whey, sugar, and wheat bran will be sought to allow the growth of LAB isolated from chicken intestine for their subsequent use in animal feed. For this purpose, 24 h fermentation kinetics were performed, taking the viability of LAB every 6 h during 24 h. It was found that the best substrate concentration where viability like the commercial culture medium was achieved was 15.76 g/L sugar, 14.24 g/L whey and 10.00 g/L soy milk, which shows that the use of alternative substrates for the elaboration of culture media can be feasible in the production of LAB.