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Vol. 6 (2012)
Published 2012-06-02
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When the Publicaciones e Investigaciones Journal receives an original study or article from its author(s), whether by email, postal service, or the platforms available for said purpose, know that it may be published in physical or electronic formats in national or international archives, databases, or SIRES. As such, Publications and Research authorizes the reproduction and citation of said material, provided that the description of information is carried out in conformity with bibliographic norms, and mention the corresponding names, authors, article, issue, and pages. Publications and Research, in advance, expresses that the information, concepts, and methods are the responsibility of the author(s). As such, the UNAD does not have any influence whatsoever over that expressed in the manuscript.

Original article

Influence of the Carbon / Nitrogen Ratio in the Production of Total Proteins in Chlorella Vulgaris UTE X 1803.

DOI: https://doi.org/10.22490/25394088.1111
Linda Estephany Durán Serrano Universidad Industrial de Santander, Bucaramanga, Colombia
Andrés Fernando Barajas Solano Universidad Industrial de Santander, Bucaramanga, Colombia.
Ángel Darío González Delgado Universidad Nacional Abierta y a Distancia
Crisóstomo Barajas Ferreira Universidad Industrial de Santander, Bucaramanga, Colombia.
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Microalgae represent a source of protein with potential applications in human and animal nutrition, since they possess a good balance of amino-acids and low content of nucleic acids in comparison with other sources of protein. However, the low productivity of cultures has prevented production at a large scale. In this work, we studied the total production of protein in Chlorella vulgaris at a laboratory scale, analyzing different pre-established parameters under the carbon/nitrogen ratio. For this, we performed mixotrophic cultures during 5 days at different concentrations of acetate (5Mm, 10Mm, 20Mm) and sodium nitrate (0,97Mm, 1,94Mm, 2,94Mm). All treatments were kept at 23 ± 1ºC, with light/dark cycles of 12h: 12h for 5 days. The highest value of protein concentration was obtained from the culture R9 with a production of 0,78±0.18 g/L, 1.7 times higher than the production of the control culture treatment. (0,46 g/L). R9 was also the culture that consumed the most nitrogen (91%). Based on these results and their analysis, we found that the total content of protein in C. vulgaris is directly proportional to the amount of acetate and nitrate present in the culture media, since the algae grown in cultures where these nutrients were limited exhibited the lowest values in terms of protein concentration

keywords: acetate, microalgae, mixotrophic culture, sodium nitrate, productivity
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How to Cite

Durán Serrano, L. E., Barajas Solano, A. F., González Delgado, Ángel D., & Barajas Ferreira, C. (2012). Influence of the Carbon / Nitrogen Ratio in the Production of Total Proteins in Chlorella Vulgaris UTE X 1803. Publicaciones E Investigación, 6, 49-58. https://doi.org/10.22490/25394088.1111
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