Efecto del factor de crecimiento fibroblástico dos (FGF-2), en la reducción de la senescencia en células madre mesenquimales aisladas de gelatina de Wharton

Andrea Catalina  Rubio Vargas; Jessica Paola  Alcázar; Laura Alejandra  Lozano Trujillo; Diana Katherine  Garzón Perdomo; Angélica Rocío  Bonilla Porras; Olivia Teresa  González Gay; Liliana Francis  Turner

doi:10.22490/24629448.6188

Vol. 20 No. 38 (2022)

Published 2022-09-13

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Research Article (before OJS)

Fibroblastic growth factor two (FGF-2), in reducing the senescent effect on mesenchymal stem cell isolated from Wharton´s jelly

DOI: https://doi.org/10.22490/24629448.6188

Andrea Catalina Rubio Vargas

Jessica Paola Alcázar

Laura Alejandra Lozano Trujillo

Diana Katherine Garzón Perdomo

Angélica Rocío Bonilla Porras

Olivia Teresa González Gay

Liliana Francis Turner

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Abstract
References

Mesenchymal stem cells have been generated interest in tissue engineering, due to their proliferative properties and tissue repair capacity, however, for a successful transplant process, it is necessary to increase the number of cells in a culture expansion process. During this process the proliferative capacity is limited, causing changes in cell morphology and functionality affecting the viability of the culture, this state is known as cell senescence. Oxidative stress and deregulation of growth factors are considered as reasons. Aims. To evaluate the effect of FGF-2 on the senescence of a mesenchymal stem cells culture isolated from Wharton´s jelly and its role in the regulation of oxidative stress. Methodology: 3,5 and 7,5 ng doses of FGF-2 were added to the culture medium from passage 2, then the senescence of the culture was evaluated and the presence of reactive oxygen species was determined during passages 5 and 7. Results. We observed that in passage 5, there is a significant difference 99.5% between the control (+) concerning the FGF-2 treatments, however, in passage 7, an increase in the production of the enzyme β-galactosidase was observed and changes in morphology such as: increase in size and elongated shape of the cell, confirming a senescent state on the culture in all the treatments evaluated. Conclusion. The doses used in this study contributed positively to decrease this process in a cell culture, also, the FGF- 2 can prolong the cultivation time, partially decreasing the concentration of reactive oxygen species.

keywords: cellular senescence, mesenchymal stem cells, oxidative stress, fibroblast growth factor 2, reactive oxygen species, Wharton jelly

Y. B. Cui et al., “Human umbilical cord mesenchymal stem cells transplantation improves cognitive function in Alzheimer’s disease mice by decreasing oxidative stress and promoting hippocampal neurogenesis,” Behav. Brain Res., vol. 320, 2017, doi: 10.1016/j.bbr.2016.12.021.

J. Vasanthan et al., “Role of Human Mesenchymal Stem Cells in Regenerative Therapy,” Cells, vol. 10, no. 1.2020, doi: 10.3390/cells10010054.

R. Yonemitsu et al., “Fibroblast Growth Factor 2 Enhances Tendon-to-Bone Healing in a Rat Rotator Cuff Repair of Chronic Tears,” Am. J. Sports Med., vol.47, no. 7, 2019, doi: 10.1177/0363546519836959.

A. Aghajani Nargesi, L. O Lerman, and A. Eirin, “Mesenchymal stem cell-derived extracellular vesicles for renal repair,” Curr. Gene Ther., vol. 17, no. 1, pp.29–42, 2017.

B. Badyra, M. Sułkowski, O. Milczarek, and M. Majka, “Mesenchymal stem cells as a multimodal treatment for nervous system diseases,” Stem Cells TranslationalMedicine, vol. 9, no. 10. 2020, doi: 10.1002/sctm.19-0430.

G. Z. Salazar Vargas, V. M. Neyra Chagua, C. R. Pitot Álvarez, A. M. Muñoz Jáuregui, and L. Á. Aguilar Mendoza, “Estudios en neurociencias: aportes para la investigación en cultivo de células madre mesenquimales,” Persona, no. 21, 2018.

J. A. Guadix, J. L. Zugaza, and P. Gálvez-Martín, “Características, aplicaciones y perspectivas de las células madre mesenquimales en terapia celular,” Medicina Clinica, vol. 148, no. 9. 2017, doi: 10.1016/j.medcli.2016.11.033.

Kobolak J, Dinnyes A, Memic A, Khademhosseini A, Mobasheri A. Mesenchymal stem cells: Identification, phenotypic characterization, biological properties and potential for regenerative medicine through biomaterial micro-engineering of their niche. Methods. 2016 Apr15;99:62-8. doi: 10.1016/j.ymeth.2015.09.016. Epub2015 Sep 15. PMID: 26384580.

M. Kosinski et al., “Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord,” Stem Cells Int., vol. 2020, 2020, doi: 10.1155/2020/1321283.

A. Can, F. T. Celikkan, and O. Cinar, “Umbilical cord mesenchymal stromal cell transplantations: a systemic analysis of clinical trials,” Cytotherapy, vol. 19, no. 12, pp.1351–1382, 2017.

M. Ziaei, J. Zhang, D. V Patel, and C. N. J. McGhee,“Umbilical cord stem cells in the treatment of corneal disease,” Surv. Ophthalmol., vol. 62, no. 6, pp. 803–815, 2017.

S. Roy, S. Arora, P. Kumari, and M. Ta, “A simple and serum-free protocol for cryopreservation of human umbilical cord as source of Wharton’s jelly mesenchymal stem cells,” Cryobiology, vol. 68, no. 3, 2014, doi:10.1016/j.cryobiol.2014.03.010.

A. C. Schnitzler et al., “Bioprocessing of human mesenchymal stem/stromal cells for therapeutic use: current technologies and challenges,” Biochem. Eng. J.,vol. 108, pp. 3–13, 2016.

S. Ramos García, “Células madre: potencial asombroso, desafiante demanda,” 2014.

Y. W. Eom et al., “The role of growth factors in maintenance of stemness in bone marrow-derived mesenchymal stem cells,” Biochem. Biophys. Res. Commun., vol. 445, no. 1, pp. 16–22, 2014.

S. H. Hong et al., “Stem cell passage affects directional migration of stem cells in electrotaxis,” Stem Cell Res., vol. 38, p. 101475, 2019.

C. Rubio-Vargas, J. Alcázar, and L. Francis-Turner, “Influencia del factor de crecimiento fibroblástico 2 en células madre in vitro Estudio de revisión.,” Actual. Biológicas, vol. 41, no. 110, 2019.

W. Wei and S. Ji, “Cellular senescence: molecular mechanisms and pathogenicity,” J. Cell. Physiol., vol. 233, no. 12, pp. 9121–9135, 2018.

V. Turinetto, E. Vitale, and C. Giachino, “Senescence in human mesenchymal stem cells: functional changes and implications in stem cell-based therapy,” Int. J. Mol. Sci., vol. 17, no. 7, p. 1164, 2016.

L. Chuaire-Noack, C. García-Morcote, and S. R.Ramírez-Clavijo, “Actividad β-galactosidasa asociadacon la senescenciChuaire-Noack, L., García-Morcote, C., & Ramírez-Clavijo, S. R. (2011). Actividad β-galactosidasa asociada con la senescencia en fibroblastos del estroma ovárico in vitro. Revista Ciencias de La Salud, 9,” Rev. Ciencias la Salud, vol. 9,no. 1, pp. 17–31, 2011.

S. Saez-Atienzar and E. Masliah, “Cellular senescence and Alzheimer disease: the egg and the chicken scenario,” Nature Reviews Neuroscience, vol. 21, no. 8.2020, doi: 10.1038/s41583-020-0325-z.

K. Endo, N. Fujita, T. Nakagawa, and R. Nishimura,“Effect of fibroblast growth factor-2 and serum oncanine mesenchymal stem cell chondrogenesis,” Tissue Eng. Part A, vol. 25, no. 11–12, pp. 901–910, 2019.

Y. Zou, H. J. Tong, M. Li, K. S. Tan, and T. Cao, “Telomere length is regulated by FGF-2 in human embryonic stem cells and affects the life span of its differentiated progenies,” Biogerontology, vol. 18, no.1, pp. 69–84, 2017.

F. Kottakis, C. Polytarchou, P. Foltopoulou, I. Sanidas, S. C. Kampranis, and P. N. Tsichlis, “FGF-2 Regulates Cell Proliferation, Migration, and Angiogenesis through an NDY1/KDM2B-miR-101-EZH2 Pathway,” Mol. Cell, vol. 43, no. 2, 2011, doi: 10.1016/j.molcel.2011.06.020.

T. Ito, R. Sawada, Y. Fujiwara, Y. Seyama, and T. Tsuchiya, “FGF-2 suppresses cellular senescence of human mesenchymal stem cells by down-regulation of TGF-β2,” Biochem. Biophys. Res. Commun., vol. 359,o. 1, pp. 108–114, 2007

L. M. de Souza, “Avaliação da indução de senescência e apoptose pelo tratamento com antraciclinas em fibroblastos humanos deficientes no reparo por excisão de nucleotídeos,” 2011.

M. Liu et al., “Adipose-derived mesenchymal stem cells from the elderly exhibit decreased migration and differentiation abilities with senescent properties,” Cell Transplant., vol. 26, no. 9, pp. 1505–1519, 2017.

W. Zhai et al., “Identification of senescent cells in multipotent mesenchymal stromal cell cultures: Current methods and future directions,” Cytotherapy, vol. 21, no. 8. 2019, doi: 10.1016/j.jcyt.2019.05.001.

X. Meng, M. Xue, P. Xu, F. Hu, B. Sun, and Z. Xiao, “MicroRNA profiling analysis revealed different cellular senescence mechanisms in human mesenchymal stem cells derived from different origin,” Genomics, vol. 109, no. 3–4, 2017, doi: 10.1016/j.ygeno.2017.02.003.

J. Franzen et al., “Senescence-associated DNA methylation is stochastically acquired in subpopulations of mesenchymal stem cells,” Aging Cell, vol. 16, no. 1, 2017, doi: 10.1111/acel.12544.

J. A. Arévalo Romero, D. Páez Guerrero, and V. M. Rodríguez Pardo, “Evaluación de características morfológicas e inmunofenotipo de células madre mesenquimales en cultivo obtenidas a partir de sangre de cordón umbilical y médula ósea.,” Nova, vol. 5, no.8, 2007, doi: 10.22490/24629448.380.32. I. Fridlyanskaya, L. Alekseenko, and N. Nikolsky,“Senescence as a general cellular response to stress: amini-review,” Exp. Gerontol., vol. 72, pp. 124–128,2015.

R. A. Avelar et al., “A multidimensional systems biology analysis of cellular senescence in aging and disease,” Genome Biol., vol. 21, no. 1, 2020, doi: 10.1186/s13059-020-01990-9.

D. N. Gala and Z. Fabian, “To Breathe or Not toBreathe: The Role of Oxygen in Bone MarrowDerived Mesenchymal Stromal Cell Senescence,” Stem Cells Int., vol. 2021, p. 8899756, 2021, doi:10.1155/2021/8899756.

J. Li et al., “Down-regulation of fibroblast growth factor 2 (FGF2) contributes to the premature senescence of mouse embryonic fibroblast,” Med. Sci. Monit., vol. 26, 2020, doi: 10.12659/MSM.920520.

U. Galderisi et al., “Efficient cultivation of neural stem cells with controlled delivery of FGF-2,” Stem Cell Res., vol. 10, no. 1, 2013, doi: 10.1016/j. scr.2012.09.001.

A. Cieślar-Pobuda, J. Yue, H.-C. Lee, M. Skonieczna, and Y.-H. Wei, “ROS and oxidative stress in stem cells.” Hindawi, 2017.

R. A. Denu and P. Hematti, “Effects of oxidative stress on mesenchymal stem cell biology,” Oxid. Med. Cell.Longev., vol. 2016, 2016.

C. L. Bigarella, R. Liang, and S. Ghaffari, “Stem cellsand the impact of ROS signaling,” Development, vol.141, no. 22, pp. 4206–4218, 2014.

Y. Liu and Q. Chen, “Senescent Mesenchymal Stem Cells: Disease Mechanism and Treatment Strategy,” Curr. Mol. Biol. Reports, vol. 6, no. 4, 2020, doi:10.1007/s40610-020-00141-0.

T. Kamiya, M. Courtney, and M. O. Laukkanen, “Redox-activated signal transduction pathways mediating cellular functions in inflammation, differentiation, degeneration, transformation, and death.” Hindawi, 2016

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How to Cite

Rubio Vargas, A. C. ., Alcázar, J. P. ., Lozano Trujillo, L. A. ., Garzón Perdomo, D. K. ., Bonilla Porras, A. R. ., González Gay, O. T. ., & Turner, L. F. . (2022). Fibroblastic growth factor two (FGF-2), in reducing the senescent effect on mesenchymal stem cell isolated from Wharton´s jelly. NOVA Biomedical Sciences Journal, 20(38), 141-157. https://doi.org/10.22490/24629448.6188

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Fibroblastic growth factor two (FGF-2), in reducing the senescent effect on mesenchymal stem cell isolated from Wharton´s jelly

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