Antagonistic activity of native Streptomyces spp. against  ESBL -and KPC- producing Enterobacteriaceae and MRSA

Víctor Manuel  Osorio; Alejandro  Mejía; Elizabeth  Correa; Ana María  Ochoa; Ana  Mercedes Rada; José  Gregorio Martínez

doi:10.22490/24629448.10141

Vol. 23 Núm. 45 (2025): 2.

Publicado 2025-07-01

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Derechos de autor 2025 Víctor Manuel Osorio, Alejandro Mejía, Elizabeth Correa, Ana María Ochoa, Ana Mercedes Rada, José Gregorio Martínez

Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.

Artículo producto de Investigación

Actividad antagónica de Streptomyces spp. nativas frente a Enterobacteriaceae productoras de BLEE y KPC, y a MRSA

DOI: https://doi.org/10.22490/24629448.10141

Víctor Manuel Osorio Institución Universitaria Colegio Mayor de Antioquia

Alejandro Mejía Institución Universitaria Colegio Mayor de Antioquia

Elizabeth Correa Institución Universitaria Colegio Mayor de Antioquia

Ana María Ochoa Institución Universitaria Colegio Mayor de Antioquia

Ana Mercedes Rada Institución Universitaria Colegio Mayor de Antioquia

José Gregorio Martínez Institución Universitaria Colegio Mayor de Antioquia

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Introducción. La búsqueda de microorganismos productores de compuestos antimicro bianos representa una estrategia para enfrentar la crisis global de la resistencia a los anti bióticos. La identificación de bacterias del género Streptomyces con actividad antagónica contra bacterias multirresistentes es el paso inicial para recuperar metabolitos antibacte rianos efectivos para inhibir estos organismos.

Objetivos. Reconocer fuentes promisorias para aislar bacterias filamentosas capaces de inhibir aislamientos clínicos incluyendo En terobacteriaceae productoras de BLEES y KPC, y MRSA.

Métodos. Se aislaron actino bacterias de rizosferas y un sistema de compostaje. Se eligieron aislamientos clínicos de MRSA, Escherichia coli, and Klebsiella pneumoniae resistentes para realizar las pruebas de antagonismo. La actividad antibacteriana se registró mediante un método de estrías cru zadas. Los aislamientos de Streptomyces se caracterizaron según el Proyecto Internacional Streptomyces y se identificaron mediante técnicas moleculares.

Resultados. Se obtuvieron nueve aislados de actinobacterias con actividad contra bacterias resistentes, dos de la rizos fera de aguacate, cuatro de un cerco vivo y tres de un sistema de compostaje; dos mostra ron actividad contra todas las bacterias resistentes evaluadas. La identificación taxonómica molecular encontró S. jumonjinensis, S. bacillaris, S. prasinus, S. microflavus y S. cadmiisoli como especies putativas para los aislados nativos.

Conclusiones. Se aislaron Streptomyces antagónicas contra Enterobacterias productoras de BLEE y KPC, y MRSA, validando el potencial de las rizosferas y los sistemas de compostaje para obtener bacterias productoras de antibióticos. Los aislados nativos presentaron características de Streptomyces; aunque el Blast de NCBI no mostró una resolución suficiente, EzBioCloud detectó la identidad de los aislados hasta especie.

Palabras clave: Actinobacterias, agentes antibacterianos, resistencia a medicamentos, bacterias productoras de betalactamasas de espectro extendido, MRSA, microbiología del suelo

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Osorio, V. M., Mejía, A., Correa, E., Ochoa, A. M., Mercedes Rada, A., & Gregorio Martínez, J. (2025). Actividad antagónica de Streptomyces spp. nativas frente a Enterobacteriaceae productoras de BLEE y KPC, y a MRSA. Revista Nova publicación científica En Ciencias biomédicas, 23(45), 7-25. https://doi.org/10.22490/24629448.10141

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Actividad antagónica de Streptomyces spp. nativas frente a Enterobacteriaceae productoras de BLEE y KPC, y a MRSA

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