Published 2008-06-15
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Research Article (before OJS)

Present Anaerobic Bacteria in Gingival Sulcus of Patients With Fixed Partial Dentures

DOI: https://doi.org/10.22490/24629448.391
José Alberto Acosta Rueda PhD Ortodoncista, Decano Facultad de Odontología Universidad Cooperativa de Colombia, Villavicencio
Diana Milena Estupiñán Torres Especialista en Odontología Legal y Forense. Docente Facultad de Odontología, Universidad Cooperativa de Colombia, Villavicencio
Geovana Katerine Reyes Guevara Periodoncista. Docente Facultad de Odontología, Universidad Cooperativa de Colombia, Villavicencio.
Liliana Sánchez Lerna Magister en Microbiología Médica, Docente Facultad de Medicina, Universidad Cooperativa de Colombia, Villavicencio.

Present anaerobic bacteria in gingival sulcus of patients with fixed partial dentures Recently, the biological aspects of fixed reconstructions have been given attention because dental materials have great capacity to accumulate and retain plaque. The microflora at both tooth and fixed partial prostheses can be identified as biofilms, which are composed of microcolonies of bacterial cells distributed in a shaped matrix. The purpose of this study was to isolate anaerobic bacteria in gingival sulcus from patients during fixed partial denture treatment. 45 samples were analyzed. 30 patients with fixed partial dentures treatment and 15 subjects without partial denture treatment were enrolled in the study. Microbiologic samples were obtained by inserting paper points into the gingival sulcus.

Samples were cultured for anaerobic bacteria on blood agar. The plates were placed into an anaerobic chamber. Later, aerotolerance tests were performed for each different colony. RapID TM ANA II System Remel was used for identification of anaerobes. A total of 12 anaerobic bacteria were observed: 8.33 % gram–negative bacilli, 58.33 % gram-positive bacilli, 0 % Gram-negative cocci, and 33.33 % gram-positive cocci. In the control group none anaerobic bacteria was isolated. Only facultative gram-positive cocci were observed (100%). The results of this study have confirmed that the presence of fixed partial dentures resulted in a change in the composition of the anaerobic microflora of the gingival sulcus, compatible with periodontal disease.

keywords: anaerobial bacteria, fixed partial dentures, gingival sulcus, periodontal disease, public health.
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How to Cite

Acosta Rueda PhD, J. A., Estupiñán Torres, D. M., Reyes Guevara, G. K., & Sánchez Lerna, L. (2008). Present Anaerobic Bacteria in Gingival Sulcus of Patients With Fixed Partial Dentures. NOVA Biomedical Sciences Journal, 6(9), 14-19. https://doi.org/10.22490/24629448.391
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