Optimización de la Reacción en Cadena de la Polimerasa para la Detección del gen B1 de T. gondii

Liliana Jazmín Cortés Cortés; Diana Carolina Hernández Castro; Mónica Mantilla; María Isabel Medina; Duque Sofía

doi:10.22490/24629448.1044

Vol. 12 No. 22 (2014)

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2014-12-15

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Optimization of Polymerase Chain Reaction for the detection of the B1 gene of T. gondii

DOI: https://doi.org/10.22490/24629448.1044

Section

Research Article (before OJS)

Liliana Jazmín Cortés Cortés Instituto Nacional de Salud

Diana Carolina Hernández Castro Instituto Nacional de Salud

Mónica Mantilla Hospital Simón Bolívar E.S.E.

María Isabel Medina Hospital Simón Bolívar E.S.E.

Duque Sofía Instituto Nacional de Salud.

Objective: This study aimed at optimizing the amplification conditions of the B1 gene (35 copies in the genome) of T. gondii in cases of possible brain toxoplasmosis. Materials and methods: DNA was extracted from the peritoneal exudate of mice inoculated with the RH strain of T. gondii. Total exudate volume was 17 mL with a concentration of 1x10⁷ parasites/mL. PCR conditions for the B1 gene were further optimized.Results: Serial dilutions from 1x10⁶ to 1x10^-1 parasites/mL were needed for amplifying a fragment of 132 bp . This set the detection limit for 1 T. gondii tachyzoite.

toxoplasmosis, optimization, polymerase chain reaction, B1 gene detection.

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Cortés Cortés, L. J., Hernández Castro, D. C., Mantilla, M., Medina, M. I., & Sofía, D. (2014). Optimization of Polymerase Chain Reaction for the detection of the B1 gene of T. gondii. NOVA Biomedical Sciences Journal, 12(22), 137-141. https://doi.org/10.22490/24629448.1044

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Optimization of Polymerase Chain Reaction for the detection of the B1 gene of T. gondii

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