Bodas de plata de la reacción en cadena de la polimerasa (PCR)

Karen Cubillos; Gladys Pinilla MSC; Monica Rodríguez

doi:10.22490/24629448.397

Vol. 6 Núm. 9 (2008)

Publicado 2008-06-15

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Artículo de Revisión

Bodas de plata de la reacción en cadena de la polimerasa (PCR)

DOI: https://doi.org/10.22490/24629448.397

Karen Cubillos Instituto de Biotecnología Universidad Nacional, Bogotá - Colombia.

Gladys Pinilla MSC Universidad Colegio Mayor de Cundinamarca, Bogotá - Colombia

Monica Rodríguez Universidad Nacional Posgrado de Microbiología

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Referencias

Invenciones verdaderamente revolucionarias han promovido el cambio de pensamiento y la manera de trabajar en el ámbito del laboratorio. Una de estas invenciones es la reacción en cadena de la polimerasa, la cual ha aportado de manera significativa al conocimiento científico. Las diferentes metodologías que aplican la reacción en cadena de la polimerasa han permitido a los investigadores manipular la información genética de los organismos, facilitando procedimientos como la clonación y la secuenciación, entre otros, lo cual agilizó significativamente los resultados del Proyecto Genoma Humano. Existe diversidad de variantes de la reacción en cadena de la polimerasa convencional. Este escrito tiene por objetivo presentar una revisión sobre el tema, especialmente sobre la reacción en cadena de la polimerasa en tiempo real, debido a las ventajas que ofrece.

Palabras clave: agentes intercalantes, cuantificación absoluta, cuantificación relativa, curvas de calibración, gen normalizador, sondas fluorogénicas.

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Cubillos, K., Pinilla MSC, G., & Rodríguez, M. (2008). Bodas de plata de la reacción en cadena de la polimerasa (PCR). Revista Nova publicación científica En Ciencias biomédicas, 6(9), 65-75. https://doi.org/10.22490/24629448.397

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