Published 2008-06-15
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Article of Review (before OJS)

Silver Anniversary of the Polymerase Chain Reaction (Pcr)

DOI: https://doi.org/10.22490/24629448.397
Karen Cubillos Instituto de Biotecnología Universidad Nacional, Bogotá - Colombia.
Gladys Pinilla MSC Universidad Colegio Mayor de Cundinamarca, Bogotá - Colombia
Monica Rodríguez Universidad Nacional Posgrado de Microbiología
Truly revolutionary inventions have promoted the change of thought and the way to work in the laboratory. One of these inventions is the polymerase chain reaction (PCR), which has contributed significantly to the scientific knowledge. The different methodologies that use the polymerase chain reaction have allowed investigators to manipulate the genetic information of organisms, facilitating procedures like cloning and sequencing, among others, which sped the Human Genome Project results significantly. There are several variants of the conventional polymerase chain reaction. This work tries to present a revision on the subject, especially on the Real-time polymerase chain reaction, due to the advantages that it offers.
keywords: absolute quantification, calibration charts, fluorescent probe, normalizing gene, intercalate agents, relative quantification.
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How to Cite
Cubillos, K., Pinilla MSC, G., & Rodríguez, M. (2008). Silver Anniversary of the Polymerase Chain Reaction (Pcr). NOVA Biomedical Sciences Journal, 6(9), 65-75. https://doi.org/10.22490/24629448.397
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