Caracterización de bacilos Gram negativos resistentes, aislados en Instituciones de Salud del Departamento de Boyacá, Colombia

Andrey Ucros-Álvarez; Sandra Galeano-Ariza; Diana López Velandia; Helena Suescún-Carrero

doi:10.22490/24629448.10561

Vol. 24 No. 46 (2026): 1

Published 2025-12-01

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Research Article (before OJS)

Characterization of resistant Gram-negative bacilli isolated in health institutions in the Department of Boyacá, Colombia

DOI: https://doi.org/10.22490/24629448.10561

Andrey Ucros-Álvarez Universidad de Boyacá

Sandra Galeano-Ariza Universidad de Boyacá

Diana López Velandia Universidad de Boyacá

Helena Suescún-Carrero Universidad de Boyacá

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Abstract
References

Introduction. Antimicrobial resistance in Gram-negative bacilli affects the global population. In the clinical context, it is associated with increased morbidity and mortality and greater use of broad-spectrum antimicrobials. Objective. To characterize resistance profiles in Gram-negative bacilli isolated from patients from healthcare institutions in the department of Boyacá during the years 2021 to 2023. Methodology. A descriptive cross-sectional study of phenotypic and genotypic characterization of carbapenemase-mediated bacterial resistance in samples from patients from healthcare institutions. Absolute and relative frequencies were established for qualitative variables, and the χ2 test was used with a significance level of 0.05. Results. A total of 183 samples with carbapenem resistance were included, with Klebsiella pneumoniae (48.6%) and Pseudomonas aeruginosa (24.0%) being the most frequently present Gram-negative bacilli among the 13 isolated species. The hospital care services from which the greatest number of isolates were obtained were the intensive care unit and hospitalization units, with urine samples being the main source of microorganisms. Genetic testing identified six types of β-lactamases: KPC, VIM, NDM, OXA-48, AmpC, and GES. A statistically significant association was found between the type of β-lactamase and the producing microorganism (p <0.05). Conclusions. The main resistance mechanism identified was the production of carbapenemases KPC, NDM, and VIM. Understanding this epidemiological profile contributes to expanding the available knowledge base, which is essential for guiding clinical decision-making in the management of infections caused by multidrug-resistant bacilli.

keywords: Klebsella, Pseudomonas, Enterobacteriaceae, beta lactam antibiotics, carbapenems, drug resistance bacterial

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Ucros-Álvarez, A., Galeano-Ariza, S., López Velandia, D., & Suescún-Carrero, H. (2025). Characterization of resistant Gram-negative bacilli isolated in health institutions in the Department of Boyacá, Colombia. NOVA Biomedical Sciences Journal, 24(46), 77-93. https://doi.org/10.22490/24629448.10561

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Characterization of resistant Gram-negative bacilli isolated in health institutions in the Department of Boyacá, Colombia

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