Publicado: 2013-06-15

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Lymphotherapy induce an increase of blocking factors and correct infertility problems

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Artículo producto de Investigación

Autores/as

Edwin Manrique
Universidad Distrital Francisco José de Caldas. Bogota, D.C. Colombia , Colombia
Verónica Rincón
Universidad Distrital Francisco José de Caldas. Bogota, D.C. Colombia , Colombia
Humberto Ossa
Laboratory of Genetic and Molecular Biology. Bogotá, D.C. Colombia. , Colombia
This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique. It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.

Lymphotherapy induce an increase of blocking factors and correct infertility problems

Autores/as

  • Edwin Manrique Universidad Distrital Francisco José de Caldas. Bogota, D.C. Colombia
  • Verónica Rincón Universidad Distrital Francisco José de Caldas. Bogota, D.C. Colombia
  • Humberto Ossa Laboratory of Genetic and Molecular Biology. Bogotá, D.C. Colombia.

DOI:

https://doi.org/10.22490/24629448.1020

Palabras clave:

lymphotherapy, blocking factors, mixed lymphocytes culture, subfertiles females rabbit and fetal allograft.

Resumen

This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique. It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.

Citas

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Publicado

2013-06-15

Cómo citar

Manrique, E., Rincón, V., & Ossa, H. (2013). Lymphotherapy induce an increase of blocking factors and correct infertility problems. Nova, 11(19), 25–31. https://doi.org/10.22490/24629448.1020

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