Lymphotherapy induce an increase of blocking factors and correct infertility problems

Edwin Manrique; Verónica Rincón; Humberto Ossa

doi:10.22490/24629448.1020

Vol. 11 No. 19 (2013)

Published 2013-06-15

license

Research Article (before OJS)

Lymphotherapy induce an increase of blocking factors and correct infertility problems

DOI: https://doi.org/10.22490/24629448.1020

Edwin Manrique Universidad Distrital Francisco José de Caldas. Bogota, D.C. Colombia

Verónica Rincón Universidad Distrital Francisco José de Caldas. Bogota, D.C. Colombia

Humberto Ossa Laboratory of Genetic and Molecular Biology. Bogotá, D.C. Colombia.

PDF (Spanish)

Abstract
References

This study aimed to confirm the presence of Blocking Factors (BFs) in Mixed Lymphocyte Culture (MLC) from female normal reproducer and sub-fertile rabbit inoculated with two injection of the allogenic lymphotherapy (LIT) to analyze its effect on rate fertility and pregnancy success. The BFs measuring was done intervening MLC with MTT-Formazan non-radioactive technique.

It was demonstrated BFs presence in MLC in female rabbit groups.. In sub-fertile female reproducers treated with allogenic lymphotherapy a significant increase in the level of FBs after every LIT was observed, as well as a rate fertility increase.. Furthermore, it was established that BFs act on cell proliferation inhibiting the MLC of other species, clearly indicating that the inhibit effect of the BFs is inter-specific and no intraspecific as had sustain until now.

keywords: lymphotherapy, blocking factors, mixed lymphocytes culture, subfertiles females rabbit and fetal allograft.

Beer A, Billingham R.E. The embryo as a transplant. In: A Scientific American. 1974; 230: 36 - 46.

Maldonado J. Efecto de la aloinmunoterapia en la reproducción animal. Tesis de grado como requisito parcial para optar al título de Magíster en Inmunología. Universidad de Antioquía. Facultad de Medicina. 1993

Ossa J, Sánchez F, Cadavid A. Inmunología de la Reproducción. Primera Edición. Imprenta Universidad de Antioquía. Colombia. 1993. 206 p.

Ossa H, Benítez A, Cárdenas S, Moreno C.H. Cuantificación de factores bloqueadores del cultivo mixto de linfocitos en mujeres abortadoras habituales tratadas con Linfoterapia. In:

Medicina Reproductive. 2000; 3(1): 33 - 36

Gurka G., Rocklin R.E. Reproductive Immunology. In: JAMA.1987;258 (20): 2983 – 7.

Lancet. Maternal blocking antibodies, the fetal allograft and recurrent abortion. The Lancet. 1983.

Mossmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and citotoxicity assays. In: Journal of Immunological Methods. 1983;65: 55 - 63.

PROMEGA. 1994. CELL TITER 96 TM Aqueous Non – Radioactive cell proliferation Assay. Technical Bulletin No 169.

PROMEGA. 1995. CELL TITER 96 TM Non – Radioactive cell proliferation Assay. Technical Bulletin No 112.

Daniel W. Bioestadística. Base para el análisis de ciencias de la salud. Editorial Uteha. Noriega editores. México. 1997; 878p.

Reyes C. 1995. Bioestadística Aplicada. Agronomía, Biología, Química. Editorial Trillas. México. 216p.

Mowbray J, Gibbings C; Liddell H; Reginald P; Underwood J; Beard R. Controlled trial of treatment of recurrent spontaneous abortion by immunisation with paternal cells. The Lancet. 1985; 8435: 941- 943.

Ober C, Karrison T, Odem R; Barnes R, Branch W; Stephenson M, Baron B, Ann M, Scott J, Schreiber J. Mononuclear cell immunisation in prevention of recurrent miscarriages: a randomised trial. The Lancet. 1999; 354: 366 - 369.

Adams C. E. Studies on prenatal mortality in the Rabbit, Oryctolagus cuniculus: The amount and distribution of loss before and after implantation. In: Journal. Endocrin. 1960;19: 325 -344.

Adams C. E. Studies on prenatal mortality in the Rabbit, Oryctolagus cuniculus: The effect of transferring varying numbers of eggs. In: Journal of Endocrin. 1962; 24: 471 - 490.

Adams C. E. Maintenance of pregnancy relative to the presence of few embryons in the rabbit. In: Journal of Endocrin. 1970; 48: 243 - 249.

Benitez A., Cardenas. Determinación de factores bloqueadores mediante el cultivo mixto de linfocitos (CML), técnica MTT. Tesis de pregrado. Universidad Distrital Francisco José de Caldas.

Scott Jr, Park M, Edwin S, Brach D. Interpretation of blocking activity in maternal serum depends on the equation used for calculation of mixed lymphocyte culture results. In: Clinical Experimental Immunology.1990;82: 363 - 368.

license

How to Cite

Manrique, E., Rincón, V., & Ossa, H. (2013). Lymphotherapy induce an increase of blocking factors and correct infertility problems. NOVA Biomedical Sciences Journal, 11(19), 25-31. https://doi.org/10.22490/24629448.1020

Download Citation

Almétricas

Metrics

File downloads

174

Declaración de privacidad.

Lymphotherapy induce an increase of blocking factors and correct infertility problems

Declaración de privacidad.