Published 2012-07-02
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Artículos de Investigación

Substances used as gelling agent alternative to the agar in culture media for in vitro propagation

DOI: https://doi.org/10.22490/21456453.972
Darío Alonso Martin Gordo Universidad Pedagógica y Tecnológica de Colombia, Tunja, Colombia
Oswaldo Cárdenas Gonzalez Universidad Pedagógica y Tecnológica de Colombia. Facultad de Ciencias. Escuela de Ciencias Químicas. Grupo de investigación Química-Física Molecular y Modelamiento Computacional (QUIMOLUPTC). Tunja. Colombia.
José Constantino Pacheco Universidad Pedagógica y Tecnológica de Colombia. Facultad de Ciencias. Escuela de Ciencias Biológicas. Grupo de investigación Bioplasma UPTC. Tunja. Colombia

The cultivation of plant fibers is achieved through the implementation of a group of techniques widely used for the propagation of plants in vitro. However, one disadvantage of these techniques is the high cost of components used for the crop’s medium, among these, the agar (primary gelling agent), has seen a price increase due to high demand. This article consults several studies regarding substances that have been utilized as alternative gelling agents, among which highlight starches (native or modified) and several plant gums and bacterial excretions that have been used in processes of organogenesis, embryogenesis and vegetative proliferation. The starches and gums studied showed favorable results in the in vitro cultivation of some plant species. The study demonstrates that both gums and starches may be potential substitutes (either partial or total) for agar, achieve a decrease in costs associated with starches, and have an ability to be chemically modified to improve gelling capacity.

keywords: agar, gelling agent, starch, gums, micro-propagation.
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How to Cite
Martin Gordo, D. A., Cárdenas Gonzalez, O., & Constantino Pacheco, J. (2012). Substances used as gelling agent alternative to the agar in culture media for in vitro propagation. Revista De Investigación Agraria Y Ambiental, 3(2), 49-62. https://doi.org/10.22490/21456453.972
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