Sustancias utilizadas como agente gelificante alternativas al agar en medios de cultivo para propagación in vitro

Darío Alonso Martin Gordo; Oswaldo Cárdenas Gonzalez; José Constantino Pacheco

doi:10.22490/21456453.972

Vol. 3 No. 2 (2012)

Published

2012-07-02

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Martin Gordo, D. A., Cárdenas Gonzalez, O., & Constantino Pacheco, J. (2012). Substances used as gelling agent alternative to the agar in culture media for in vitro propagation. Revista De Investigación Agraria Y Ambiental, 3(2), 49-62. https://doi.org/10.22490/21456453.972

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Substances used as gelling agent alternative to the agar in culture media for in vitro propagation

DOI: https://doi.org/10.22490/21456453.972

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Artículos de Investigación

Darío Alonso Martin Gordo Universidad Pedagógica y Tecnológica de Colombia, Tunja, Colombia

Oswaldo Cárdenas Gonzalez Universidad Pedagógica y Tecnológica de Colombia. Facultad de Ciencias. Escuela de Ciencias Químicas. Grupo de investigación Química-Física Molecular y Modelamiento Computacional (QUIMOLUPTC). Tunja. Colombia.

José Constantino Pacheco Universidad Pedagógica y Tecnológica de Colombia. Facultad de Ciencias. Escuela de Ciencias Biológicas. Grupo de investigación Bioplasma UPTC. Tunja. Colombia

The cultivation of plant fibers is achieved through the implementation of a group of techniques widely used for the propagation of plants in vitro. However, one disadvantage of these techniques is the high cost of components used for the crop’s medium, among these, the agar (primary gelling agent), has seen a price increase due to high demand. This article consults several studies regarding substances that have been utilized as alternative gelling agents, among which highlight starches (native or modified) and several plant gums and bacterial excretions that have been used in processes of organogenesis, embryogenesis and vegetative proliferation. The starches and gums studied showed favorable results in the in vitro cultivation of some plant species. The study demonstrates that both gums and starches may be potential substitutes (either partial or total) for agar, achieve a decrease in costs associated with starches, and have an ability to be chemically modified to improve gelling capacity.

agar, gelling agent, starch, gums, micro-propagation.

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Substances used as gelling agent alternative to the agar in culture media for in vitro propagation

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