Published 2014-01-07
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Artículos de Investigación

Evaluation of the effect of disinfection treatments with sodium hypochlorite over nodal segments present in Guadua angustifolia Kunth for the establishment of the in vitro culture.

DOI: https://doi.org/10.22490/21456453.944
Lorena Alexandra Ramírez Correa Corporación Autónoma Regional del Quindío. Centro Nacional para el Estudio del Bambú – Guadua. Calle 19 Norte N° 19-55. Armenia, Quindío. Colombia
Jairo Enrique Granados Moreno Universidad Nacional Abierta y a Distancia
Nidia Elizabeth Carreño González Universidad Nacional Abierta y a Distancia

The establishment of the in vitro culture of Guadua angustifolia nodal segments presents as the main problem the contamination by microorganisms, causing biological and economic losses. This research was developed at the National Center for the Study of the bamboo-Guadua bamboo, in municipality of Cordoba, Quindio and it was financed by the Regional Autonomous Corporation of the Quindio (C. R. Q). Six treatments were evaluated for the disinfection of the explants of guadua bamboo with sodium hypochlorite (NaClO) in concentrations of 2% and 3 %, each one of them in time of application of 5, 10, and 15 minutes , the explants were planted in the culture medium Murashige and Skoog medium supplemented with 6-BAP at a rate of 3mg L-1. The percentage of sprouting was also evaluated. The best result of disinfection and budding was obtained with the NaClO to 2% for 15 minutes.

keywords: Guadua angustifolia, explant, in vitro culture, sodium hypochlorite, disinfection, sprouting.
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How to Cite

Ramírez Correa, L. A., Granados Moreno, J. E., & Carreño González, N. E. (2014). Evaluation of the effect of disinfection treatments with sodium hypochlorite over nodal segments present in Guadua angustifolia Kunth for the establishment of the in vitro culture. Revista De Investigación Agraria Y Ambiental, 5(1), 155-169. https://doi.org/10.22490/21456453.944
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