Producción de dos cepas de Frankia sp. aisladas de Alnus acuminata H.B.K. por fermentación fed-batch

Ana María Rey Obando; Rolando Barahona Rosales; Diego Rosendo Chamorro Viveros

doi:10.22490/21456453.925

Vol. 5 No. 1 (2014)

Published

2014-01-07

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Production of two strains of Frankia sp. isolated from Alnus acuminata H. B. K. using fed-batch fermentation

DOI: https://doi.org/10.22490/21456453.925

Section

Artículos de Investigación

Ana María Rey Obando Universidad Cooperativa de Colombia. Pasto

Rolando Barahona Rosales Universidad Nacional de Colombia. Medellín

Diego Rosendo Chamorro Viveros Universidad Nacional Abierta y a Distancia UNAD. Medellín.

Nitrogen, one of the most important factors in the agricultural production systems, it is provides to the ground through chemical fertilization, natural phenomena and biological fixation by soil microorganisms as Frankia, a mechanism environmentally and economically viable. The use of Frankia as inoculant requires a thorough study related to the development and analysis of the behavior of the inocula in controlled conditions. Assessments were carried out focused on study and standardize the conditions that allow the development of a prototype of inoculant to base of Frankia strains Aan17, Aac49 and eca AacIII Chilean, prior to its production to large scale. It was determined the nutritional conditions that influence the growth and formation of specialized structures of Frankia in a fermentation fed-batch system on the scale of a liter. The dynamics of growth in the media evaluated showed highly significant differences (P< 0.001) observing the highest specific growth rate (0,025 h-1) and shorter doubling time (33.9 h) in the medium M1 root extract, yeast and glucose. With the average M3 root extract, yeast and molasses, it was noted the widest length of time of the exponential phase (64.86 h), the highest specific speed of substrate consumption (0,007 ) and the largest cellular performance based on the substrate (1.95 g/protein/substrate). It was evidenced that the nutritional components evaluated presented influence on the production of biomass (P<0.05 ), showing higher biomass production in regard to the reference medium Denzil Amina Purina (BAP) (P< 0.01 ).

microorganisms, diazotrofos, inoculants, fermentation

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Rey Obando, A. M., Barahona Rosales, R., & Chamorro Viveros, D. R. (2014). Production of two strains of Frankia sp. isolated from Alnus acuminata H. B. K. using fed-batch fermentation. Revista De Investigación Agraria Y Ambiental, 5(1), 81-92. https://doi.org/10.22490/21456453.925

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Production of two strains of Frankia sp. isolated from Alnus acuminata H. B. K. using fed-batch fermentation

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