Optimización de protocolo de establecimiento in vitro de Magnolia arcabucoana (Lozano) Govaerts. (Magnoliaceae) como una herramienta de conservación

Diana Milena Avendaño Torres; Elberth Hernando Pinzón Sandoval

doi:10.22490/21456453.7651

Vol. 16 No. 1 (2025)

Published 2024-12-19

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Optimization the in vitro establishment protocol of Magnolia arcabucoana (Lozano) Govaerts. (Magnoliacea) as a conservation tool

DOI: https://doi.org/10.22490/21456453.7651

Diana Milena Avendaño Torres Universidad Pedagógica y Tecnologica de Colombia

Elberth Hernando Pinzón Sandoval Universidad Pedagógica y Tecnológica de Colombia

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Abstract
References

Contextualization: Magnolia arcabucoana (Lozano) Govaerts, is a species categorized as endangered, with deficient sexual propagation due to low fruit and seed production, which generates a high vulnerability of existing populations to extinction events. In vitro tissue culture techniques are an effective alternative for the propagation of threat species that require specific strategies for their conservation.

Knowledge gap: At present, there is no protocol that creates optimal conditions to enable the disinfection and in vitro establishment of Magnolia arcabucoana.

Purpose: Establish and optimize a protocol for the disinfection and in vitro establishment of vegetative buds as a conservation tool for the species.

Methodology: Three experimental trials were conducted corresponding to a disinfection protocol, phenol inhibitor agents, and establishment. DCA experimental designs and existing methodologies for each protocol were employed.

Results and conclusions: It was obtained that concentrations of 1 g L-1 of CA (Activated Carbon) + 0.5 g L-1 of AA (Ascorbic Acid) included in the culture medium and AA solution at 25 % w/v for 15 minutes, 67.7 % controlled phenolic oxidation and bud necrosis. The best disinfection treatment was achieved with inversion for three minutes in a concentration of 0.8 % of Ca(ClO)2. In the establishment phase, the greatest growth of the buds was achieved with Woody Plant Medium (WPM) with 1 mg L-1 of BAP (6 Benzyl Aminopurine) + 0.05 mg L-1 of ANA (Naphthaleneacetic acid). Sprouting was obtained 105 days after establishment in the WPM medium with 3 mg L-1 of BAP. The results constitute a contribution for the in vitro establishment of Magnolia arcabucoana for conservation purposes

keywords: Biotechnology, culture medium, Direct organogenesis, Growth regulators, Phenolic oxidation

Abdin, M. Z., Kiran, U., Kamaluddin, & Ali, A. (2017). Plant biotechnology: Principles and applications. In Plant Biotechnology: Principles and Applications. https://doi.org/10.1007/978-981-10-2961-5

Aguilar-Cano, J., Mendoza-Cifuentes, H., & Ayala-Joya, M. (2018). Dos nuevas especies de árboles molinillo (Magnolia: Magnoliaceae) de la Serranía de los Yariguíes, departamento de Santander, Colombia. Biota Colombiana, 19(s1), 27–42. https://doi.org/10.21068/c2018.v19s1a04

Ahmad, I., Hussain, T., Ashraf, I., Nafes, M., Maryam., Rafay, M., & Iqbal, M. (2013). Lethal effects of secondary metabolites on plant tissue culture. In Amer. Eur. J. Agric. Environ. Sci. (Vol. 13, pp. 539–547). http://www.idosi.org/aejaes/jaes13(4)13/14.pdf

Amente, G., & Chimdessa, E. (2021). Control of browning in plant tissue culture: A review. Journal of Scientific Agriculture, 5, 67–71. https://doi.org/10.25081/jsa.2021.v5.7266

Araújo, M. da C. da R., Chagas, E. A., Vendrame, W., Ribeiro, M. I. G., Moura, E. A. de, Taveira, D. L. L., Chagas, P. C., & Grigio, M. L. (2021). Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. Crop Breeding and Applied Biotechnology, 21(3). https://doi.org/10.1590/1984-70332021v21n3a40

Avendaño Torres, D. M., Pinzón Sandoval, E. H., & Serrano Cely, P. A. (2022). Aspectos biotecnológicos en la propagación in vitro de magnoliáceas. Ciencia y Agricultura, 19(3), 116–131. https://doi.org/10.19053/01228420.v19.n3.2022.15195

Azofeifa-Delgado, Á. (2008). Problemas de oxidación y oscurecimiento de explantes cultivados in vitro. Agronomía Mesoamericana, 20(1), 153. https://doi.org/10.15517/am.v20i1.4990

Blokhina, O., Virolainen, E., & Fagerstedt, K. V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: A review. Annals of Botany, 91, 179–194. https://doi.org/10.1093/aob/mcf118

Borah, R., Kumaria, S., & Choudhury, H. (2017). In vitro plant regeneration of magnolia punduana: An endemic and threatened plant species. Plant Tissue Culture and Biotechnology, 27(2), 153–159. https://doi.org/10.3329/ptcb.v27i2.35020

Cardozo, S., Marin, B. S., Godoy, J., & Suarez, S. (2017). Propagación in vitro de Magnolia Hernandezii (Molinillo) a partir de hojas. Revista de La Asociación Colombiana de Ciencias Biológicas, 29, 80–85.

Chávez-Cortázar, A., Oyama, K., Ochoa-Zavala, M., Mata-Rosas, M., Veltjen, E., Samain, M. S., & Quesada, M. (2021). Conservation genetics of relict tropical species of Magnolia (section Macrophylla). Conservation Genetics, 22(2), 259–273. https://doi.org/10.1007/s10592-021-01334-5

Coelho, N., Gonçalves, S., & Romano, A. (2020). Endemic plant species conservation: Biotechnological approaches. Plants, 9(3). https://doi.org/10.3390/plants9030345

Cuba-Díaz, M., Acuña, D., Cordero, C., & Klagges, M. (2014). Optimización de parámetros para la propagación in vitro de Colobanthus quitensis (Kunth) Bartl. Gayana Bot., 71(1), 58–67.

Cui, Y., Deng, Y., Zheng, K., Hu, X., Zhu, M., Deng, X., & Xi, R. (2019). An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers. Scientific Reports, 9(1), 1–10. https://doi.org/10.1038/s41598-019-46050-w

Díaz-Lezcano, M., Rodas-Ramirez, J., Gonzalez-Segnana, L., & Ortiz, M. (2021). Control de la oxidación fenólica de segmentos nodales de Handroanthus heptaphyllus en condiciones in vitro. Cedamaz, 11(1), 1–5.

Gutiérrez-Lozano, M., Sánchez-González, A., Vázquez-García, J. A., López-Mata, L., & Octavio-Aguilar, P. (2020). Population morphological differentiation of Magnolia rzedowskiana (Magnoliaceae): Endemic species in danger of extinction of the Sierra Madre Oriental, Mexico. Revista Mexicana de Biodiversidad, 91. https://doi.org/10.22201/IB.20078706E.2020.91.3101

Hernández, Y., & González, M. (2010). Efectos de la contaminación microbiana y oxidación perennes. Cultivos Tropicales, 31(4), 58–69.

IUCN. (2022). Guidelines for Using the IUCN Red List Categories and Criteria. IUCN Standards and Petitions Committee. https://www.iucnredlist.org/documents/RedListGuidelines.pdf.

Kang, L., Zheng, K., Xie, Y., Deng, Y., Yu, Y., Zhu, M., Xi, R., & Deng, X. (2020). Efficient tissue culture protocol for magnolia lucida (Magnoliaceae) and confirmation of genetic stability of the regenerated plants. Plants, 9(8), 1–12. https://doi.org/10.3390/plants9080997

Kulak, V., Longboat, S., Brunet, N. D., Shukla, M., & Saxena, P. (2022). In Vitro Technology in Plant Conservation: Relevance to Biocultural Diversity. Plants, 11(4), 1–20. https://doi.org/10.3390/plants11040503

Lee, Y. J., Lee, Y. M., Lee, C. K., Jung, J. K., Han, S. B., & Hong, J. T. (2011). Therapeutic applications of compounds in the Magnolia family. Pharmacology and Therapeutics, 130(2), 157–176. https://doi.org/10.1016/j.pharmthera.2011.01.010

Luo, Y., Zheng, K., Liu, X., Tao, J., Sun, X., Deng, Y., & Deng, X. (2023). In Vitro Propagation and Genetic Uniformity Assessment of Manglietiastrum sinicum: A Critically Endangered Magnoliaceae Species. Plants, 12(13), 2500. https://doi.org/10.3390/plants12132500

Maestro, R., & Borja, R. (1993). Actividad antioxidante de las vitaminas C y E y de la provitamina A. Grasas y Aceites, 44(2), 107–111. https://doi.org/10.3989/gya.1993.v44.i2.1106

Palmarola, A., Granado, L., Albelo, N., Testé, E., Hernández, M., & González-Torres, L. (2018). Population structure and distribution of Magnolia cubensis subsp . acunae. Revista Del Jardín Botánico Nacional, 39, 103–111.

Palmarola, A., Simón, R., Testé, E., Hernández, M., Sosa, A., Molina, Y., & González-Torres, L. R. (2020). Distribution and conservation of Magnolia (Magnoliaceae) in cuba. Botanical Sciences, 1(1), 300–313. https://doi.org/10.17129/botsci.2868

Parris, J. K., Touchell, D. H., Ranney, T. G., & Adelberg, J. (2012). Basal Salt Composition, Cytokinins, and Phenolic Binding Agents Influence In Vitro Growth and Ex Vitro Establishment of Magnolia ‘Ann.’ HortScience, 47(11), 1625–1629. https://doi.org/10.21273/HORTSCI.47.11.1625

Pedroza, J. A. (2009). Efecto del carbón activado , ácido indolacético (AIA) y bencil amino purina (BAP) en el desarrollo de protocormos de Epidendrum elongatum Jacq bajo condiciones in vitro. Revista Colombiana de Biotecnologia, 11(1), 17–32.

Poniewozik, M., Parzymies, M., & Szot, P. (2022). Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne. Horticultural Science, 49(1), 48–51. https://doi.org/10.17221/68/2020-HORTSCI

R Core Team. (2022). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.r-project.org/.

Radomir, A.-M. (2012). Comparative study on the in vitro multiplication potential of Magnolia stellata and Magnolia x soulangiana species. Journal of Horticulture, Forestry and Biotechnology, 16(2), 39–44.

Rivers, M., Beech, E., Murphy, L., & Oldfield, S. (2016). The Red List of Magnoliaceae - revised and extended. In The Red List (BGCI. Rich, Issue March). BGCI. Richmond, UK.

Rodríguez-Duque, D. L., Escobar-Alba, M., García-González, J. D., Carvajal-Cogollo, J. E., & Aymard-Corredor, G. A. (2023). A New Andean Species of Magnolia (Section Talauma, Magnolioideae, Magnoliaceae), and a Key to the Species Found in Colombia. Harvard Papers in Botany, 27(2), 3350. https://doi.org/10.3100/hpib.v27iss2.2022.n1

Serna-González, M., Urrego-Giraldo, L. E., Santa-Ceballos, J. P., & Suzuki-Azuma, H. (2022). Flowering, floral visitors and climatic drivers of reproductive phenology of two endangered magnolias from neotropical Andean forests. Plant Species Biology, 37(1), 20–37. https://doi.org/10.1111/1442-1984.12351

Serna-González, M., & Velásquez-Ruiz, C. (2017). Pollen of colombian magnolias. Caldasia, 39(1), 59. https://doi.org/10.15446/caldasia.v39n1.64315

Sokolov, R. S., Atanassova, B. Y., & Iakimova, E. T. (2014). Physiological response of in vitro cultured Magnolia Sp. to nutrient medium composition. Journal of Horticultural Research, 22(1), 49–61. https://doi.org/10.2478/johr-2014-0006

Thomas, T. D. (2008). The role of activated charcoal in plant tissue culture. Biotechnology Advances, 26(6), 618–631. https://doi.org/10.1016/j.biotechadv.2008.08.003

Vázquez-García, J. A., Neill, D. A., Azanza, M., Pérez, Á. J., Dahua-Machoa, A., Merino-Santi, E., Delgado-Chaves, A. F., & Urbano-Apraez, S. M. (2017). Magnolia mindoensis (subsect. Talauma, Magnoliaceae): Una especie nueva del Chocó biogeográfico premontano en Colombia y Ecuador. Brittonia, 69(2), 197–208. https://doi.org/10.1007/s12228-016-9449-x

Wojtania, A., Dziurka, M., & Skrzypek, E. (2020). In vitro rooting response of yellow-flowered magnolia in relation to the phenolic acids content. Agronomy, 10(12), 1–12. https://doi.org/10.3390/agronomy10121880

Wojtania, A., Skrzypek, E., Gabryszewska, E., & Sci Pol, A. (2015). Effect of cytokinin, sucrose and nitrogen salts concentrations on the growth and development and phenolics content in Magnolia × soulangiana ‘Coates’ shoots In vitro. Acta Sci. Pol. Hortorum Cultus, 14(3), 51–62.

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Avendaño Torres, D. M., & Pinzón Sandoval, E. H. (2024). Optimization the in vitro establishment protocol of Magnolia arcabucoana (Lozano) Govaerts. (Magnoliacea) as a conservation tool. Revista De Investigación Agraria Y Ambiental, 16(1), 103-122. https://doi.org/10.22490/21456453.7651

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