Caracterización molecular con marcadores ISSR de la colección de cítricos de la Universidad de los Llanos

Christian Camilo Castañeda Cardona; Rogelio  Portela Puerta; Yacenia Morillo Coronado

doi:10.22490/21456453.3884

Vol. 12 No. 2 (2021)

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2021-06-13

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Molecular characterization with ISSR markers of the citrus collection from the Universidad de los Llanos

DOI: https://doi.org/10.22490/21456453.3884

Section

Área Agrícola

Castañeda-Cardona, C. Universidad de los Llanos

Portela-Puerta, R. Universidad de los Llanos

Morillo-Coronado, Y. AGROSAVIA

Contextualization: Citrus trees are one of the most cultivated tree species in the world. They are of great importance since their production is greater than that of any other fruit tree.

Knowledge gap: Despite the great demand for citrus fruits in Colombia, there is limited knowledge about the origin and genetic diversity. Likewise, molecular characterization studies of citrus varieties established in the work collection of the Universidad de los Llanos have not been carried out, which is of vital importance to implement conservation strategies and potential use of genetic resources.

Purpose: to evaluate the genetic diversity of four citrus varieties (Tangelo orange, Valencia orange, Arrayana mandarin and Castilla lemon), established at the University of the Llanos with seven ISSR primers.

Methodology: The molecular characterization was carried out in the Plant Biotechnology and Animal Reproduction and Genetics laboratories of the Universidad de los Llanos Universidad de los Llanos. A binary matrix of absence and presence was generated. The genetic similarity between the individuals was calculated using the coefficient of similarity of Nei and Li (1979). The cluster analysis was performed by the UPGMA method and a dendrogram was generated using the NTSYS statistical package, version 2.02 pc. The unbiased heterozygosity, the percentage of polymorphic loci, and the unbiased f statistic were estimated with a 95% confidence interval, using the TFPGA statistical package, version 1.3.

Results and conclusions: A total of 80 bands were obtained, of which 86.25% were polymorphic. The average estimated heterozygosity for the total population was 0.29, which shows a moderate genetic diversity. The CGA and AG primers were the ones with the greatest contribution to the estimation of genetic polymorphism. Little genetic differentiation was found (Fst = 0.03). At a level of similarity of 0.42, seven groups were formed, with groups 1 and 2 being the ones that grouped the largest number of genotypes of the four species, being mostly Arrayana mandarin and Tangelo orange. The seven primers were significant for the estimation of genetic diversity in citrus fruits and constitute a tool with great potential for further improvement work in this species.

Citrus, Genetic Differentiation, Germplasm, Microsatellite, Genetic Variability

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Castañeda Cardona, C. C., Portela Puerta, R. ., & Morillo Coronado, Y. (2021). Molecular characterization with ISSR markers of the citrus collection from the Universidad de los Llanos. Revista De Investigación Agraria Y Ambiental, 12(2), 67-84. https://doi.org/10.22490/21456453.3884

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