Isolamento de lectinas por cromatografia de afinidade

José Camilo Torres Romero; Myriam Janeth Ortega Torres; Jhon Alexander Infante Betancour; Cicero Antonio Maia Cavalcante

doi:10.22490/21456453.1837

Vol. 8 Núm. 1 (2017)

Publicado 13-01-2017

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Derechos de autor 2017 Revista de Investigación Agraria y Ambiental

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Artículos de Investigación

Isolamento de lectinas por cromatografia de afinidade

DOI: https://doi.org/10.22490/21456453.1837

José Camilo Torres Romero Universidad Nacional Abierta y a Distancia

Myriam Janeth Ortega Torres Universidad Nacional Abierta y a Distancia

Jhon Alexander Infante Betancour YOLUKA ONG Fundación de Investigación en Biodiversidad y Conservación. 4Instituto Federal do Ceará – IFCE. Jaguaribe –Ceará, Brasil.

Cicero Antonio Maia Cavalcante Instituto Federal do Ceará – IFCE. Jaguaribe –Ceará, Brasil.

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Cromatografia de afinidade é uma técnica usada para separar compostos, como, por exemplo, determinadas proteínas, que têm a capacidade de se ligar não covalentemente e reversivelmente a moléculas específicas conhecidas como ligantes. Esse método difere das técnicas de cromatografia clássica devido a proteína conseguir ser separada com base em uma única propriedade bioquímica. Em cromatografia de afinidade, o ligante está ligado covalentemente à matriz, que deve ser quimicamente inerte, porosa e também ter uma variedade de grupos funcionais adequados para acoplamento com ligantes diferentes. Várias matrizes e ligantes são usados em cromatografia de afinidade, dependendo da proteína a ser purificada. Esse trabalho descreverá alguns aspectos importantes para o isolamento de lectinas através da técnica de cromatografia de afinidade utilizando-se carboidratos.

Palabras clave: proteínas, lectinas, carboidratos, isolectinas, cromatografia.

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Torres Romero, J. C., Ortega Torres, M. J., Infante Betancour, J. A., & Maia Cavalcante, C. A. (2017). Isolamento de lectinas por cromatografia de afinidade. Revista De Investigación Agraria Y Ambiental, 8(1), 63-69. https://doi.org/10.22490/21456453.1837

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