Estudio de Biomoléculas con Actividad Inhibitoria sobre Helicobacter pylori en Pacientes Colombianos con Alto Riesgo de Cáncer Gástrico

Diana Marcela  Burbano Vasquez; Carlos David  López Miramag; Lizeth Camila  Maigual Enríquez; Yudy Banesa  Legarda Nastul; Oscar  Arango Bedoya; Andres Mauricio  Hurtado Benavides; Alvaro Jairo  Pazos Moncayo

doi:10.22490/24629448.9981

Vol. 23 No. 44 (2025): 1.

Published 2025-07-01

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Copyright (c) 2025 Diana Marcela Burbano Vasquez, Carlos David López Miramag, Lizeth Camila Maigual Enríquez, Yudy Banesa Legarda Nastul, Oscar Arango Bedoya, Andres Mauricio Hurtado Benavides, Alvaro Jairo Pazos Moncayo

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Study of Biomolecules with Inhibitory Activity on Helicobacter pylori in Colombian Patients with High Risk of Gastric Cancer

DOI: https://doi.org/10.22490/24629448.9981

Diana Marcela Burbano Vasquez Universidad de Nariño

Carlos David López Miramag Universidad de Nariño

Lizeth Camila Maigual Enríquez Universidad del Cauca

Yudy Banesa Legarda Nastul Universidad de Nariño

Oscar Arango Bedoya Universidad de Nariño

Andres Mauricio Hurtado Benavides Universidad de Nariño

Alvaro Jairo Pazos Moncayo Universidad de Nariño

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Abstract
References

Introduction: Gastric cancer in Nariño represents a significant public health problem due to its high incidence and mortality rates. Eradicating Helicobacter pylori is a key strategy for preventing this disease, but the failure of some treatments has prompted the search for alternatives, such as the use of natural compounds. Among these, Brassica oleracea var. Itálica and Cannabis sativa extracts rich in cannabidiol stand out. Objective: To evaluate the in vitro inhibitory activity of broccoli and Cannabis sativa extracts rich in cannabidiol on the growth of Helicobacter pylori isolated from patients with gastric disease in Nariño. Methodology: Helicobacter pyloriwas isolated and purified from gastric biopsies of patients from the Department of Nariño. Brassica oleraceavar. Itálica extracts, with high sulforaphane content, were obtained using rotaevaporation and lyophilization techniques. The Cannabis sativa extract was obtained using supercritical CO₂ extraction. The inhibitory activity of the extracts was evaluated using the agar dilution method, colony-forming units (CFU) were counted using ImageJ software, and the minimum inhibitory concentration (MIC) for each isolate was determined. Results:The MIC of Brassica oleracea var. Itálica extracts with high sulforaphane content was 120 µg/mL. The MIC of the Cannabis sativa extract was 55 µg/mL. Conclusion: Brassica oleracea var. Itálica and Cannabis sativaextracts rich in cannabidiol exhibit an inhibitory effect on the in vitro growth of Helicobacter pylori isolated from patients in Nariño, with the Cannabis sativa extract being the most effective.

keywords: Gastric cancer, extracts, Helicobacter pylori, Cannabis sativa L, Brassica oleracea, sulforaphane, minimum inhibitory concentration

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Burbano Vasquez, D. M. ., López Miramag, C. D. ., Maigual Enríquez, L. C. ., Legarda Nastul, Y. B., Arango Bedoya, O. ., Hurtado Benavides, A. M. ., & Pazos Moncayo, A. J. . (2025). Study of Biomolecules with Inhibitory Activity on Helicobacter pylori in Colombian Patients with High Risk of Gastric Cancer. NOVA Biomedical Sciences Journal, 23(44), 35-49. https://doi.org/10.22490/24629448.9981

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Study of Biomolecules with Inhibitory Activity on Helicobacter pylori in Colombian Patients with High Risk of Gastric Cancer

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