Identificación de polimorfismos en el gen mieloperoxidasa y su papel en candidiasis vulvovaginal recurrente por Candida spp., aisladas de pacientes de Bogotá DC

María Paula  Montoya; Gloria Julieth  Rincón; Julián Esteban  Muñoz; Sharon Hassbleidy  Ochoa; Claudia  Andrea Cruz; Jeanette  Navarrete

doi:10.22490/24629448.8198

Vol. 22 No. 42 (2024): Primer número 2024

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2024-06-22

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Identification of polymorphisms in the myeloperoxidase gene and its role in recurrent vulvovaginal candidiasis due to Candida spp., in patients from Bogotá DC.

DOI: https://doi.org/10.22490/24629448.8198

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Article (before OJS)

María Paula Montoya https://orcid.org/0000-0002-6970-7704

Gloria Julieth Rincón

Julián Esteban Muñoz https://orcid.org/0000-0003-4495-7077

Sharon Hassbleidy Ochoa

Claudia Andrea Cruz https://orcid.org/0000-0003-1041-9609

Jeanette Navarrete https://orcid.org/0000-0003-1935-7125

Introduction: Myeloperoxidase (MPO) is an antimicrobial protein present in the azuro- philic granules of neutrophils, its action falls when it is conjugated in the phagosome with hydrogen peroxide, resulting in toxic substances for phagocytosed microorganisms. Seve- ral authors have described that recurrent vulvovaginal infections by Candida spp., are rela- ted to primary MPO deficiency, which is an autosomal recessive disorder, due to polymor- phisms associated with posttranslational processing or pre-translational errors. Objective: To identify the relationship between the two MPO polymorphisms (Y173C-M251T) with a higher probability of presenting vulvovaginal infection. Materials and methods: 27 blood samples were collected from women between 20-45 years old diagnosed with vul- vovaginal candidiasis in order to analyze the complete blood count, Wright stain, leuko- cyte peroxidase stain and perform DNA extraction to assess the quantity and quality of genetic material. Subsequently, primers for the mpo gene were designed by bioinformatic tools and the conditions to standardize conventional PCR were established. Amplified products were sequenced by capillary electrophoresis (CES) to identify polymorphisms. Results: The polymorphisms of interest were not found in any sample. However, nucleoti- de changes were found in exons 4 and 6 of the fragment studied in two samples analyzed, indicating that this region may present genetic variability. Conclusion: The present study is a first approximation in Colombia on the mpo gene polymorphisms from patients with vulvovaginal Candidiasis in Bogotá D.C. Future studies with a more representative num- ber of samples will give us stronger indications of the genetic variability that the mpo gene may present.

Polymorphisms, Myeloperoxidase Deficiency, PCR, Sequencing, Vulvovaginal Candidiasis, Candida spp.

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Montoya, M. P. ., Rincón , G. J. ., Muñoz, J. E. ., Ochoa , S. H. ., Andrea Cruz, C., & Navarrete, J. . (2024). Identification of polymorphisms in the myeloperoxidase gene and its role in recurrent vulvovaginal candidiasis due to Candida spp., in patients from Bogotá DC. NOVA Biomedical Sciences Journal, 22(42). https://doi.org/10.22490/24629448.8198

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