Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

Oscar Gabriel  Beltrán; Ligia Denise  Torres Higuera; José Luis  Rodríguez Bautista; Rocio Esperanza  Patiño Burbano

doi:10.22490/24629448.6585

Vol. 20 No. 39 (2022)

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2022-12-21

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Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

DOI: https://doi.org/10.22490/24629448.6585

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Research Article (before OJS)

Oscar Gabriel Beltrán Universidad Colegio Mayor de Cundinamarca

Ligia Denise Torres Higuera Universidad Colegio Mayor de Cundinamarca

José Luis Rodríguez Bautista Universidade Federal Rural do Rio de Janeiro

Rocio Esperanza Patiño Burbano Corporación colombiana de investigación agropecuaria-AGROSAVIA

Objective. The genetic stability of Strains of Leptospira spp., maintained under two conservation systems, was evaluated. Methodology. The degree of conservation of the 16S rRNA and ompL1 genes of 10 reference serovars from the Leptospira spp. collection, belonging to the Sistema de Bancos de Germoplasma de la Nación para la Alimentación y la Agricultura (SBGNAA), was determined. Results. It was corroborated that the genes evaluated these have not undergone considerable changes, since similarities greater than 99.69 % were evidenced for 16S rRNA and 99.02% for ompL1, in the paired alignments. Conclusion. The genetic stability and purity of the reference strains of Leptospira spp. were verified. spp., kept in cryopreservation in liquid nitrogen at -196 °C and at room temperature for approximately eight years.

sequence analysis, DNA, ompL1 protein, Leptospira, RNA, Ribosomal, 16S

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Beltrán, O. G. ., Torres Higuera, L. D. ., Rodríguez Bautista, J. L. ., & Patiño Burbano, R. E. . (2022). Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods. NOVA Biomedical Sciences Journal, 20(39), 65-79. https://doi.org/10.22490/24629448.6585

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