Detección de anticuerpos tipo IgG contra Borrelia  Burgdorferi, y factores asociados a la enfermedad de Lyme  en población canina, de los municipios Honda-Tolima,  La Mesa y Chia-Cundinamarca

Lucia Constanza  Corrales; Stiven  Quijano Duarte; Evelyn  Ramírez Hern´ández

doi:10.22490/24629448.6180

Vol. 20 No. 38 (2022)

Published

2022-09-13

PDF (Spanish)

Metrics

Metrics Loading ...

Detection of IgG-type antibodies against Borrelia Burgdorferi, and factors associated with Lyme disease in the canine population, of the municipalities Honda-Tolima, La Mesa and Chia-Cundinamarca

DOI: https://doi.org/10.22490/24629448.6180

Section

Research Article (before OJS)

Lucia Constanza Corrales

Stiven Quijano Duarte

Evelyn Ramírez Hern´ández

Borrelia burgdorferi, is the spirochete responsible for causing Lyme disease in man and different animals. Objective. Detect specific IgG type antibodies against Borrelia burgdorferi, in canines using the immunofluorescence technique and its correlation with other factors associated with Lyme disease. Methods. Blood was taken for IgG detection against Borrelia burgdorferi sl; Peripheral blood smear of the canines and hemolymph of the ticks to search for spirochetes with Wright staining and finally classification of the ticks using morphometric keys. Results. In the serological test, on average 69.0% of the canines sampled gave positive results in the different titrations. Bacterial structures were spirally visualized both in the peripheral blood of the canines and in the hemolymph of the ticks. The vector found was classified as Rhipicephalus sanguineus, until now not reported in the scientific literature as a carrier of Borrelia, nor associated with the disease.

Borrelia burgdorferi, Lyme disease, spirochete, Erythema, dogs, immunofluorescence

Leschnik M, Canine borreliosis: Are we facing the facts? J.med.vet.mycol. [Internet] 2013; 2:197-198. [Consultado 2018 nov 18]. Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/24316153

García M, Skinner C, Salas J, Ocampo J. Enfermedad de Lyme: Actualizaciones. Gac.méd. Méx. [Internet] 2014; 150:84-95 [Consultado 2019 ene 20]. Disponible en: https://www.anmm.org.mx/GMM/2014/n1/GMM_150_2014_1_084-095.pdf

Corrales L, Ávila S, Estupiñan S. Bacteriología Teoría y Práctica. Bogotá, D.C. Editorial Universidad Colegio Mayor de Cundinamarca;2013.

Gomes D, Costa R, Wischral T, Santos R, Tamekuni K, Rodríguez N et al. Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil. Rev. bras. parasitol. vet. [Internet]. 2016; 25 (4): 418-422. [Consultado 2018 nov 18]. Disponible en: http://www.scielo.br/pdf/rbpv/v25n4/1984-2961-rbpv-S1984-29612016085.pdf

Tinoco L, Quiroz H, Quintero M, Renteria T, Barreras A, López G, Hori S, Tamayo A, et al. Seroprevalence of borrelia burgdorferi in dogs from a Mexico-U.S. Border deser región: pilot study. J. anim. vet. adv. [Internet] 2007; 6: 787-789. [Consultado 2019 ene 22] Disponible en: http://docsdrive.com/pdfs/medwelljournals/javaa/2007/787-789.pdf

Campos C. El sistema inmunológico en los mamíferos: Las defensas del cuerpo. Rev.biol. trop. [internet] 2014;8 (1) :80-93 [Consultado 2018 feb 10] Disponible: https://revistas.ucr.ac.cr/index.php/nutrianimal/article/view/14921

Bautista C, Mosqueda J. Papel de los receptores tipo Toll en la inmunidad innata y su complicación en medicina veterinaria. Vet. Méx. [Internet] 2005; 36 (4): 453-468 [Consultado 2018 feb 10] Disponible en: https://www.redalyc.org/articulo.oa?id=42336407

Girardin S, Travassos L, Herve M, Blanot D, Boneca I, Philpott D, et al. Peptidoglycan Molecular Requirements Allowing Detection by Nod1 and Nod2. J. Biol. Chem. [Internet] 2003, 278:41702-41708. [Consultado 2018 feb 10] Disponible en: http://www.jbc.org/content/278/43/41702.full.html#ref-list-1

Collado V, Porras R, Cutili M, Gómez E. El sistema inmune innato I: sus mecanismos. RCCV. [Internet] 2008 2(1): 1-16. [Consultado 2018 feb 10] Disponible en: http://revistas.ucm.es/index.php/RCCV/article/view/RCCV0808120001A/22569

Tizard I. Inmunología veterinaria. 10a ed. BarcelonaEspaña: Saunders Elsevier. 2019.11. Meryl P. Littman, VMD. Canine Borreliosis. Vet. Med. Small anim. clin. [Internet] 2003;33: 827–862 [Consultado 2018 nov 18]. Disponible en: http://www.vetsmall.theclinics.com/article/S0195-5616(03)00037-8/abstract

Caride Villaamil E. epidemiología de Borrelia Burgdorferi s.l. (Enfermedad de Lyme) en un ecosistema de pinar de montaña supramediterraneo. [Tesis doctoral en Veterinaria] Madrid: Universidad Complutense de Madrid. Facultad de veterinaria. Departamento de Patología Animal; 2002.

Rodríguez I. Actualización acerca de Borrelia burgdorferisensu lato y enfermedad de lyme. Rev. cuba. med. trop. [Internet] 2013; 65(2): 149-165. [Consultado 2019 ene 20]. Disponible en: http://scielo.sld.cu/pdf/mtr/v65n2/mtr02213.pdf

Orestes L, Infante J, Ramírez C, Lavastida H. Enfermedad de Lyme: Historia, microbiología, epizootiología y epidemiologia. Rev. cuba. hig. epidemiol. [Internet] 2011; 50(2): 231-244 [Consultado 2019 ene 22]. Disponible en: http://scielo.sld.cu/pdf/hie/v50n2/hie12212.pdf

The center for food security and public health. Enfermedad de Lyme [Internet]. [Consultado 2019 ene 22]. Disponible en: http://www.cfsph.iastate.edu/DiseaseInfo/disease.php?name=lyme-disease&lang=es

Jacobson R, Chang Y, Shin S. Lyme disease: laboratory diagnosis of infected and vaccinated symptomatic dogs. Semin Vet Med Surg [Internet] 1996; 11 (3): 172-182 [Consultado 2019 feb 12]. Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/8942214

Aguero-Rosenfeld M, Wang G, Schwartz I, Wormser G. Diagnosis of Lyme Borreliosis. Clin Microbiol Rev. [Internet] 2005;18(3):484-509. [Consultado 2019 ene 20]. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1195970/pdf/0038-04.pdf

Brisson D, Drecktrah D, Eggers C, Samuels D. Genetics of Borrelia burgdorferi. Ann.rev.genet. [Internet] 2012; 46 :513–34. [Consultado 2019 mar 17] Disponible en: https://www.annualreviews.org/doi/abs/10.1146/annurevgenet-011112-112140

Casjens S, Mongodin E, Qiu W, Luft B, Schutzer S, Gilcrease E. et al. Genome Stability of Lyme Disease Spirochetes: Comparative Genomics of Borrelia burgdorferi Plasmids. Plos ONE. [Internet] 2012; 7 (3). [Consultado 2019 mar 17] Disponible en: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0033280

Lagal V, Postic D, Ruzic-Sabljic E, Baranton G. Genetic Diversity among Borrelia Strains Determined by SingleStrand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness. J. clin. microbiol. [Internet] 2003; 41 (11): 5059–5065. [Consultado 2019 mar 17] Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC262544/

Marconi R, Samuels D, Garon C. Transcriptional Analyses and Mapping of the ospC Gene in Lyme Disease Spirochetes. J. Bacteriol. [Internet] 1993;175(4): 926–932. [Consultado 2019 mar 17] Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC193003/

Chaconas G, Kobryn K. Structure, Function, and Evolution of Linear Replicons in Borrelia. Annu. Rev. Microbiol. [Internet] 2010; 64:185–202. [Consultado 2019 mar 17] Disponible en: https://www.annualreviews.org/doi/abs/10.1146/annurev.micro.112408.134037?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=micro

Lin T, Gao L, Edmondson D, Jacobs M, Philipp M, Norris S. Central Role of the Holliday Junction Helicase RuvAB in vlsE Recombination and Infectivity of Borrelia burgdorferi. Plos ONE. [Internet] 2009; 5 (12). [Consultado 2019 mar 17] Disponible en: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1000679

Radolf J, Calimano M, Stevenson B, T.Hu L. Of ticks, mice and men: understanding the dual-host lifestyle of Lyme disease spirochaetes. Nat. Rev. Microbiol. [Internet] 2012 10(2): 87–99. [Consultado 2019 mar 17] Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/22230951

Zhang J, Hardham J, Barbour A, Norris S. Antigenic Variation in Lyme Disease Borreliae by Promiscuous Recombination of VMP-like Sequence Cassettes. Cell. [Internet] 1997; 89: 275-285. [Consultado 2019 mar 17] Disponible en: https://www.ncbi.nlm.nih.gov/pubmed/9108482

Dresser A, Hardy P, Chaconas G. Investigation of the Genes Involved in Antigenic Switching at the vlsE Locus in Borrelia burgdorferi: An Essential Role for the RuvAB Branch Migrase. PLoS Pathog. [Internet] 2009; 5 (12). [Consultado 2019 mar 17] Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779866/

Cruz N, Mayoral P. Borreliosis, fiebre recurrente causada por espiroquetas. Informe de un caso. Bol. méd. Hosp. Infant. Méx. [Internet] 2012;69(2):121-125 [Consultado 2019 mar 20] Disponible en: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1665-11462012000200008

Boria B. Enfermedad de Lyme: presentación de casos clínicos y conocimiento de la enfermedad entre veterinarios y estudiantes en los municipios de Veracruz y Boca del Rio. [Tesis de grado para obtención de título de Médico Veterinario Zootecnista] Veracruz: Universidad Veracruzana. Facultad de Medicina Veterinaria y zootecnia; 2012.

Osorio G. Búsqueda de la espiroqueta Borrelia burgdorferi sensu lato mediante PCR en garrapatas ixoideas chilenas silvestres. Rev. méd. Chile. [Internet] 2001;129(3). [Consultado 2019 mar 20] Disponible en: https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0034-98872001000300006

Corrales, L. C. ., Quijano Duarte, S. ., & Ramírez Hern´ández, E. . (2022). Detection of IgG-type antibodies against Borrelia Burgdorferi, and factors associated with Lyme disease in the canine population, of the municipalities Honda-Tolima, La Mesa and Chia-Cundinamarca. NOVA Biomedical Sciences Journal, 20(38), 11-35. https://doi.org/10.22490/24629448.6180

Download Citation

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Published

Detection of IgG-type antibodies against Borrelia Burgdorferi, and factors associated with Lyme disease in the canine population, of the municipalities Honda-Tolima, La Mesa and Chia-Cundinamarca

Declaración de privacidad.

Keywords