Copyright (c) 2021 NOVA Biomedical Sciences Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
NOVA por http://www.unicolmayor.edu.co/publicaciones/index.php/nova se distribuye bajo una licencia Reconocimiento No Comercial- Compartir igual
Así mismo, los autores mantienen sus derechos de propiedad intelectual sobre los artículos,
Declaración de privacidad.
Los nombres y las direcciones de correo electrónico introducidos en esta revista se usarán exclusivamente para los fines establecidos en ella y no se proporcionarán a terceros o para su uso con otros fines.
Astaxanthin production under stress factors using a 5L laboratory scale bioreactor
Introduction. Haematococcus pluvialis is a microalgae that produces astaxanthin, a beta-carotene and antioxidant widely used in industry. In order to obtain a higher production of astaxanthin, the Objective was to use different stress factors, in a 5-liter laboratory-scale bioreactor. Methodology. The microalgae was cultivated in the RM medium, pH 6.8, temperature 20 ± 2ºC, filtered air, illumination with white lamps 20h light/4h darkness, irradiance 70 μE m − 2s − 1, different concentrations of sodium acetate and chloride of sodium. Cell growth, morphological changes and quantification of astaxanthin and chlorophyll were determined by spectrophotometry. Statistical analysis was performed using ANOVA (95%).
Results. Using 0.299 mg/L of sodium acetate a cell growth of 2.0 x 104 Cel/mL and an astaxanthin concentration of 2.530 µg/mL were obtained, while with 1.6 mg/L of sodium acetate the cell growth It was 3.5 x 104 Cel/mL and an astaxanthin concentration of 1.9 µg/mL. The treatment to which 1.6 g L of sodium acetate and 6.4 g/L of sodium chloride were added showed the highest astaxanthin production, 7.3 µg/ml. Treatment with 0.320 g/L sodium acetate + 1.28 g/L sodium chloride showed the highest cell growth with 1.64x105 cells/ml. Conclusion. This research highlights the importance of initially cultivating the microalgae using the 5-liter Tecferm bioreactor and, after its exponential phase, subjecting it to stress factors with sodium acetate and sodium chloride, thus achieving the highest production of 7.325 µg / ml astaxanthin.
How to Cite
