Modelo Animal de Anemia Inducida por Flebotomía Crónica: Relación Funcional Entre Hierro y Eritropoyesis

Martha Castillo Bohórquez MSC; María D´Anna; Suani Gaona Prieto; Betina García; Gisela Giorgi; Ana Isabel Mora Bautista MSC; Marta Roque; María del Mar Villarraga Muñoz

doi:10.22490/24629448.517

Vol. 10 No. 17 (2012)

Published

2012-06-15

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Castillo Bohórquez MSC, M., D´Anna, M., Gaona Prieto, S., García, B., Giorgi, G., Mora Bautista MSC, A. I., Roque, M., & Villarraga Muñoz, M. del M. (2012). Modelo Animal de Anemia Inducida por Flebotomía Crónica: Relación Funcional Entre Hierro y Eritropoyesis. NOVA Biomedical Sciences Journal, 10(17), 50-63. https://doi.org/10.22490/24629448.517

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Modelo Animal de Anemia Inducida por Flebotomía Crónica: Relación Funcional Entre Hierro y Eritropoyesis

DOI: https://doi.org/10.22490/24629448.517

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Research Article (before OJS)

Martha Castillo Bohórquez MSC Universidad Colegio Mayor de Cundinamarca

María D´Anna Universidad Nacional del Sur. Bahía Blanca.

Suani Gaona Prieto Universidad Colegio Mayor de Cundinamarca

Betina García Universidad Nacional del Sur. Bahía Blanca.

Gisela Giorgi Universidad Nacional del Sur. Bahía Blanca.

Ana Isabel Mora Bautista MSC Universidad Colegio Mayor de Cundinamarca

Marta Roque Universidad Nacional del Sur. Bahía Blanca.

María del Mar Villarraga Muñoz Universidad Colegio Mayor de Cundinamarca

In the present work we studied the erythropoietic response to chronic phlebotomy-induced anemia and changes in cellular and systemic body iron distribution. Female mice (CF1 strain, n =32) were divided into control and experimental groups, following a paired experimental design. Iron distribution in spleen and liver during chronic phlebotomy was assessed by morphological studies and erythropoietic activity by hematological studies. Statistical differences were determined by Student test. The significance level was set at p <0.05. During chronic phlebotomy we observed a significantly decreased of hemoglobin as an anemia indicator and reticulocytosis as an indicator of erythron restoration. Adult mouse spleen was the main tissue that contributed available iron to the erythron, being evident splenic iron depletion. The study of chronic induced anemia in mice allowed us to the development of patho physiological conditions similar to those observed in human pathologies. The model of chronic phlebotomy was useful to evaluate the erythropoiesis in the setting of anemia and its recovery, correlating with the distribution of an essential nutrient such as iron.

Anemia, chronic phlebotomy, erythropoiesis, hematological studies

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