Evaluación de la prueba ELISA usando una cepa de campo como antígeno, para detectar anticuerpos contra el virus de la Rinotraqueitis Bovina Infecciosa

Jeannette Navarrete MSC; Víctor Vera, PhD; Gloria Ramírez; Luis Carlos Villamil PhD

doi:10.22490/24629448.5

Vol. 2 No. 2 (2004)

Published

2004-12-15

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Navarrete MSC, J., Vera, PhD, V., Ramírez, G., & Villamil PhD, L. C. (2004). Evaluation of Elisa Test Use of Field Strain (Antigen) to Detect Antibodies Against Infectious Bovine Rhinotracheitis Virus. NOVA Biomedical Sciences Journal, 2(2), 33-39. https://doi.org/10.22490/24629448.5

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Evaluation of Elisa Test Use of Field Strain (Antigen) to Detect Antibodies Against Infectious Bovine Rhinotracheitis Virus

DOI: https://doi.org/10.22490/24629448.5

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Jeannette Navarrete MSC Universidad Colegio Mayor de Cundinamarca

Víctor Vera, PhD Facultad de Medicina Veterinaria y Zootecnia, Programa de Postgrado. Universidad Nacional de Colombia

Gloria Ramírez Facultad de Medicina Veterinaria y Zootecnia, Programa de Postgrado. Universidad Nacional de Colombia

Luis Carlos Villamil PhD Facultad de Medicina Veterinaria y Zootecnia, Programa de Postgrado. Universidad Nacional de Colombia

The following study set up an enzymatic immune test (ELISA) using viral particles broke up of field strain of IBR virus; it showed a high sensibility and specificity and low rate of false negatives and positives. Positive and negative bovine serum which ELISA technique was normalized, were analyzed by seroneutralization to determine anti IBR antibodies; It was collected positive and negative serum with titles 1:4 to 1:128 and was considered dilution of bovine serum 1:50 as optimal dilution to ELISA technique.

On the other hand, coefficient of variation BR was used to analyze the rehearsing of absorbance obtained by positive and negative serum. The results obtained were less than 20%, which indicated an appropriate trustiness and a low coefficient of variation. Sensibility and specificity found was 96% and 98% respectively, taking as a reference test the seroneutralization. The results obtained determined that ELISA test with viral particles broke up of field strain detected anti IBR antibodies with a high sensibility and specificity, being an improvement tool to diagnose the illness.

ELISA, seroneutralization, infectious bovine rhinotracheitis, antibodies, diagnostic enhancement

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Evaluation of Elisa Test Use of Field Strain (Antigen) to Detect Antibodies Against Infectious Bovine Rhinotracheitis Virus

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