Adaptación de bacterias a diferentes concentraciones de fenol en el laboratorio: aspectos esenciales para un proceso de biodegradación

Gustavo Eugenio Echeverri Jaramillo

doi:10.22490/24629448.489

Vol. 9 No. 15 (2011)

Published 2011-06-15

license

Research Article (before OJS)

Adaptation of Bacteria to Different Concentrations of Phenol in the Laboratory: Essentials for Biodegradation Process

DOI: https://doi.org/10.22490/24629448.489

Gustavo Eugenio Echeverri Jaramillo Universidad San Buenaventura-Cartagena

PDF (Spanish)

Abstract
References

It was intended to see growth of bacterial load of contaminated samples, based on adaptation to different concentrations of phenol. Were followed in the middle mineralized concentrations (200 to10000mg/L), and variations of inoculum. Demonstrated changes in bacterial growth, increased burden found in soil, with phase adjustment on the second day and growth phase on the third day, most clearly in 200 and 500mg/L, decreasing with increasing concentration. Comparing variations in the inoculums (directly adapted and pre-enrichment), the samples can be applied to direct high load (soil), the pre-enrichment is not practical for bacterial stress, can be used and adapted to 500mg/L with no problems. In ringing on agar (mineralized and nutritious), there was growth up to 1000mg/ L, making it clear toal low detailed study biochemical characterization. It is important to purify waste water from different sources to find bacteria that resist high concentrations of phenol and allow effective ness in bioprocesses, knowing preadaptation behaviors, such as study time and appropriate treatment for the preparation of inoculum.

keywords: biodegradation of phenol, bioremediation, environmental biotechnology, degrading bacteria, marine environment.

Chi-Wen, L., Chia-Hsien, Y.,Shen-Long.T.Biotreatment of phenolcontaminated wastewater in a spiral packed-bed bioreactor. Bioprocess BiosystEng.2009(32):575–580.

Jiang, H,TayJand Tay, S.Aggregation of immobilized activated sludge cells into aerobically grown microbial granules for the aerobic biodegradation of phenol. Letters in Applied Microbiology. 2002(35): 439–445.

Gurusamy, A., Ruey-Shin, J. and Duu-Jong, L. Biodegradation and adsorption ofphenol using activated carbon immobilized with Pseudomonas putida. J. Environ. Sci. Health, A. 2002(37):1133–1146.

Begonña,M, Hidalgo, A, Serra, JL, Llama, MJ. Degradation of phenol by RhodococcuserythropolisUPV-1 immobilized on Biolite® in a packed-bed reactor.Journal of Biotechnology. 2002(97): 1–11.

Godjevargova, T,Ivanova, D, Alexieva, Z, Dimova, N. Biodegradation of toxic organic components from industrialphenol production waste waters by free and immobilizedTrichosporoncutaneum R57. Process Biochemistry. 2003(38) 915-/920.

Diomi, M., Emmanuel, K., Nikolaos, P., Dimitris G., Hatzinikolaou, P. Biodegradation of Phenol by Acclimatized Pseudomonas putida Cells Using Glucose as an Added Growth Substrate. Journal of Environmental Science and Health Part A—Toxic/Hazardous Substances & Environmental Engineering. 2004 (39): 2093–2104.

Arutchelvan,V, Kanakasabai, V, Elangovan,R,Nagarajan, S,Muralikrishnan, V.Kinetics of high strength phenol degradation using Bacillus brevis.Journal of Hazardous Materials B. 2006(129) 216–222.

Vidya,K, Kalifathulla, I, Srinikethan,G.Performance of pulsed plate bioreactor for biodegradation of phenol. Journal of Hazardous Materials. 2007(140):346–352.

Dipty, S., Fulekar,MH. Bioremediation of Phenol Using Microbial Consortium InBioreactor.Innovative Romanian Food Biotechnology (2007): 1-11.

Ruiz, N, Ruiz, JC, Castañón, JH, Hernández, E, Cristiani, E, y Galíndez, J.PhenolBiodegradationUsing a RepeatedBatch Culture ofCandidatropicalisin a MultistageBubbleColumn. RevistaLatinoamericana de Microbiología2001(43):19-25.

Khaled,M.Biodegradation of Phenol by Actinobacillus Sp.: Mathematical Interpretation and effect of some growth condititions.Bioremediation Journal. 2007 (11):103-12.

Kyung Han Kwon Æ Sung Ho Yeom. Optimal microbial adaptation routes for the rapid degradation of high concentration of phenol. BioprocessBiosystEng (2009) 32:435–442.

Morlett, JA, Ascacio, JA, Rivas, AM, Velázquez, JF,Haskins, W, Barrera, HA, Acuña, K. Kinetics of BTEX biodegradation by a microbial consortium acclimatized to unleaded gasoline and bacterial strains isolated from it. International Biodeterioration & Biodegradation. 2010(64): 581-587.

Yan, J, Jianping, W, Jing, B, Xiaoqiang, J, Zongding, H. Biodegradation of phenol at high initial concentration by Alcaligenesfaecalis.Journal of HazardousMaterials.2007(147): 672–676.

Vidya,K., Ramanjaneyulu, R, Srinikethan,G.Biological phenol removal using immobilized cells in a pulsed plate bioreactor: Effect of dilution rate and influent phenol concentration.Journal of Hazardous Materials. (2007b).

Echeverri, G. et al. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la bahía de Cartagena, Colombia. Revista Nova. 2011( 8): 92-102.

Jiangya, Z, Xiaojuan, Y, Cong, D, Zhiping W, Qianqian, Z, Hao, P, Weimin, C. Optimization of phenol degradation by Candida tropicalisZ-04 usingPlackett-Burman design and response surface methodology. Journal of Environmental Sciences. 2011(23):22–30.

Plackett-Burman.Design and Response SurfaceMethodology. Journal of Environmental Sciences. 2011( 23) 22–30.

license

How to Cite

Echeverri Jaramillo, G. E. (2011). Adaptation of Bacteria to Different Concentrations of Phenol in the Laboratory: Essentials for Biodegradation Process. NOVA Biomedical Sciences Journal, 9(15), 60-69. https://doi.org/10.22490/24629448.489

Download Citation

Almétricas

Metrics

Metrics Loading ...

Declaración de privacidad.

Adaptation of Bacteria to Different Concentrations of Phenol in the Laboratory: Essentials for Biodegradation Process

How to Cite