Colonización por Staphylococcus aureus en una población de pacientes VIH positivos de la ciudad de Medellín: perfil de sensibilidad antimicrobiana y caracterización de la resistencia a la meticilina

Luz Astrid Velásquez; Diana Marcela Sánchez; Orville Hernández; Andrés González; Diana Henao; Ángela Perez; Clara María Duque Restrepo

doi:10.22490/24629448.445

Vol. 8 No. 14 (2010)

Published 2010-12-15

license

Research Article (before OJS)

Colonization By in an Hiv-Positive Patient Population of the City of Medellín: Antimicrobial Susceptibility Patterns and Characterization of Resistance to Methicillin

DOI: https://doi.org/10.22490/24629448.445

Luz Astrid Velásquez Grupo Biociencias Institución Universitaria Colegio Mayor de Antioquia

Diana Marcela Sánchez Grupo Biociencias Institución Universitaria Colegio Mayor de Antioquia

Orville Hernández Grupo Biociencias Institución Universitaria Colegio Mayor de Antioquia

Andrés González Dinámica IPS

Diana Henao Estudiantes Institución Universitaria Colegio Mayor de Antioquia

Ángela Perez Estudiantes Institución Universitaria Colegio Mayor de Antioquia

Clara María Duque Restrepo Grupo Biociencias Institución Universitaria Colegio Mayor de Antioquia

PDF (Spanish)

Abstract
References

To determine the prevalence of nasal colonization of S Staphylococcus aureus, its antimicrobial sensitivity profile, methicillin-resistance in strains isolated and the relation between carrier state, immunological status and use of antimicrobials in a group of patients infected with human immunodeficiency virus (HIV) in Medellin.

151 nasal samples were obtained from ambulatory patients with HIV. Antimicrobial sensitivity profile of S. aureus isolates were evaluated using the Kirby-Bauer method. In addition, we also determined minimum inhibitory concentrations (MIC) for oxacillin and vancomycin. Presence of the mecA gene in methicillin-resistant S. aureus isolates (MRSA) was confirmed through polymerase chain reaction.

keywords: Staphylococcus aureus, methicillin resistance, colonization, human immunodeficience virus and PCR.

Velázquez Meza ME. Surgimiento y diseminación de Staphylococcus aureus Meticilinorresistente. Salud Publica Mex 2005; 47: 381-387.

Daza Pérez R.M. Resistencia bacteriana a antimicrobianos: su importancia en la toma de decisiones en la práctica diaria. Inf Ter Sist Nac alud 1998; 22: 57-67.

Justos JA, Handan A, Gutierrez M. Staphylococcus aureus: La Reemergencia de un Patógeno en la comunidad. 2006 Rev Biomed 17 (4): 287-305.

Echevarria Zarate J, Iglesias Quilca D. Estafilococo Meticilinorresistente, un problema actual en la emergencia de resistencia entre los Gram positivos. Rev Med Hered 2003; 14 (4): 195-199 Rev

Koneman EW. Capitulo11 parte I: Estafilococos y microorganismos relacionados. En: Diagnostico Microbiológico Texto y Atlas Color. Montevideo, Uruguay: Editorial médica panamericana, 2004: 528-540

Tenover FC, Lancaster MV, Hill B, Steward CD. Characterization of Staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J Clin Microbiol. 1998; 36: 1020-7.

Po-Liang Lu, Lien-Chun Chin, Chien-Fang Peng, Yi-Hsiung Chiang, Tyen-Po Chen, Ling Ma, L. K Siu. Risk factors and molecular analysis of community methicillin-resistant staphylococcus aureus carriage. Journal of clinical microbiology 2005; 43 (1):132–139.

Cortes JA, Gómez CA, Cuervo SI, Leal AL, GREBO. Communityacquired methicillin-resistant Staphylococcus aureus in Bogotá, Colombia: Public Health implications. Revista de salud pública, 2007; 9 (3): 448-454.

Carleton H. A Ten Year Survey of Staphylococcus aureus (Saur) Isolates Causing Infections Among Gay Men and People with HIV in San Francisco: ICAAC San Francisco Septiembre 2006. pg. 123 Abstract C2-1142

Wiiliamson JC. Community-Acquired MRSA (CA-MRSA) Infection Among HIV-Positive Patients Is Associated with Active Viral Replication: ICAAC San Francisco Septiembre 2006. pg. 364 Abstract K-1674

A. Uche. Outcomes of MRSA Bacteremia in HIV Infected Patients: ICAAC San Francisco Septiembre 2006. pg. 324 Abstract K-779

Safdar N, Narans L, Gordon B, Maki DG. Comparison of Culture Screening Methods for Detection Of Nasal Carriage of Methicillin- Resistant Staphylococcus aureus: a Prospective Study Comparing 32 Methods. J Clin Microb. 2003 41 (7): 3163-3166.

Jaffe RI, Lane JD, Albury SV, Niemeyer DM. rapid Extraction from and Direct Identification in Clinical samples of Methicillin-resistant Staphylococcus aureus Using the PCR. J Clin Microb. 2000 38 (9): 2407 3412

Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. CLSI document M 100-S17

J.S Villacian, T.Barkham, A.Earnest, N.I. Paton. Prevalence of and Risk Factors for Nasal Colonization with Sthaphylococcus aureus Among Human Immunodeficiency Virus- Positive Outpatients in Singapore. Infect Control Hosp Epidemiol 2004;25:438-440.

M. Amir, J. Paul, B. Batchelor, S. Kariuki, J. Ojoo, R Waiyaki and C. Gilks. Nasopharyngeal Carriage of Staphylococcus aureus and Carriage of Tetracycline-Resistant Strains Associated with HIV-Seropositivity. Eur. J. Clin. Microbiol. Infect. Dis., 1995, 14:34-40.

L Clifford McDonald, Tsai-Ling Lauderdale, Hsiu-Jung Lo, Jih-Jin Tsai and Chien-Ching Hung. Colonization of HIV-infected outpatients in Taiwan with methicillin-resistant and methicillin-susceptible Staphylococcus aureus. Int J STD AIDS.2003; 14: 473-477

license

How to Cite

Velásquez, L. A., Sánchez, D. M., Hernández, O., González, A., Henao, D., Perez, Ángela, & Duque Restrepo, C. M. (2010). Colonization By in an Hiv-Positive Patient Population of the City of Medellín: Antimicrobial Susceptibility Patterns and Characterization of Resistance to Methicillin. NOVA Biomedical Sciences Journal, 8(14), 133-139. https://doi.org/10.22490/24629448.445

Download Citation

Almétricas

Declaración de privacidad.

Colonization By in an Hiv-Positive Patient Population of the City of Medellín: Antimicrobial Susceptibility Patterns and Characterization of Resistance to Methicillin

How to Cite