Cuantificación de ADN libre en plasma sanguíneo de voluntarios sanos en una población bogotana

Jairo Amaya Guio; Fabio Ancizar Aristizabal Gutiérrez; Ignacio Briceño Balcázar; Marcos Castillo Zamora; Dabeiba Adriana García Robayo; Hernán David Salazar Jordan

doi:10.22490/24629448.427

Vol. 7 No. 12 (2009)

Published

2009-12-15

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Amaya Guio, J., Aristizabal Gutiérrez, F. A., Briceño Balcázar, I., Castillo Zamora, M., García Robayo, D. A., & Salazar Jordan, H. D. (2009). Quantification of free DNA in blood plasma of healthy volunteers in Bogotá population. NOVA Biomedical Sciences Journal, 7(12), 136-142. https://doi.org/10.22490/24629448.427

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Quantification of free DNA in blood plasma of healthy volunteers in Bogotá population

DOI: https://doi.org/10.22490/24629448.427

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Research Article (before OJS)

Jairo Amaya Guio Hospital de Engativá, Departamento de Ginecología

Fabio Ancizar Aristizabal Gutiérrez Universidad Nacional de Colombia, Departamento de Farmacia

Ignacio Briceño Balcázar Universidad de La Sabana, Facultad de Medicina

Marcos Castillo Zamora Dinámica – IPS, Departamento de Colposcopia. Universidad de La Sabana, Facultad de Medicina

Dabeiba Adriana García Robayo Pontificia Universidad Javeriana, Centro de Investigaciones Odontológicas

Hernán David Salazar Jordan Pontificia Universidad Javeriana, Departamento de Biología

The free DNA in blood increases with some pathological conditions and certain physiological state. Several reports in the literature have highlighted that the free DNA in plasma or serum has clinical potential as a possible tool for the prognosis of human cancer. However, so far there are no reference values of healthy individuals with a representative sample size and no values have been reported for specific populations such as Bogota. That is why in this study we quantified the concentration of free DNA in healthy people of the Bogota population and thus established a normal range or reference value, the relationship between levels of free DNA and characteristics such as age and gender was further analyzed. The concentration of free DNA in the Bogota population was 0.72 ng/μL and no significant differences between ages and genders were found.

ALU repeats, free DNA, real-time PCR, reference value.

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Quantification of free DNA in blood plasma of healthy volunteers in Bogotá population

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