Cuantificación de ADN libre en plasma sanguíneo de voluntarios sanos en una población bogotana

Jairo Amaya Guio; Fabio Ancizar Aristizabal Gutiérrez; Ignacio Briceño Balcázar; Marcos Castillo Zamora; Dabeiba Adriana García Robayo; Hernán David Salazar Jordan

doi:10.22490/24629448.427

Vol. 7 No. 12 (2009)

Published 2009-12-15

license

Research Article (before OJS)

Quantification of free DNA in blood plasma of healthy volunteers in Bogotá population

DOI: https://doi.org/10.22490/24629448.427

Jairo Amaya Guio Hospital de Engativá, Departamento de Ginecología

Fabio Ancizar Aristizabal Gutiérrez Universidad Nacional de Colombia, Departamento de Farmacia

Ignacio Briceño Balcázar Universidad de La Sabana, Facultad de Medicina

Marcos Castillo Zamora Dinámica – IPS, Departamento de Colposcopia. Universidad de La Sabana, Facultad de Medicina

Dabeiba Adriana García Robayo Pontificia Universidad Javeriana, Centro de Investigaciones Odontológicas

Hernán David Salazar Jordan Pontificia Universidad Javeriana, Departamento de Biología

PDF (Spanish)

Abstract
References

The free DNA in blood increases with some pathological conditions and certain physiological state. Several reports in the literature have highlighted that the free DNA in plasma or serum has clinical potential as a possible tool for the prognosis of human cancer. However, so far there are no reference values of healthy individuals with a representative sample size and no values have been reported for specific populations such as Bogota. That is why in this study we quantified the concentration of free DNA in healthy people of the Bogota population and thus established a normal range or reference value, the relationship between levels of free DNA and characteristics such as age and gender was further analyzed. The concentration of free DNA in the Bogota population was 0.72 ng/μL and no significant differences between ages and genders were found.

keywords: ALU repeats, free DNA, real-time PCR, reference value.

Giacona MB, Ruben GC, Iczkowski KA, Roos TB, Porter DM, Sorenson GD. Cell-Free DNA in human blood plasma: length measurements in patients with pancreatic cancer and healthy controls. Pancreas. 1998;17:89-97.

Jung M, Klotzek S, Lewandowski M, Fleischhacker M, Jung K. Changes in concentration of DNA in serum and plasma during storage of blood samples. Clin Chem. 2003;49:1028-1029.

Stemmer C, Beau-Faller M, Pencreac’h E, Guerin E, Schneider A, Jaqmin D, Quoix E, Gabú MP, Oudet P. Use of magnetic beads for plasma cell-free DNA extraction: toward automation of plasma DNA analysis for molecular diagnostics. Clin Chem. 2003;49:1953-1955.

Sozzi G, Conte D, Leon ME, Cirincione R, Roz L, Ratcliffe C, Roz L, Cirenei N, Bellomi M, Pelosi G, Pierotti MA, Pastorino U. Quantification of free circulating DNA as a diagnostic marker in lung cancer. J Clin Oncol. 2003;21:3902 3908.

Pathak AK, Bhutani M, Kumar S, Mohan A, Guleria R. Circulating cell-free DNA in plasma/serum of lung cancer patients as a potential screening and prognostic tool. Clin Chem. 2006;52:1833-1842.

Jahr S, Hentze H, Englisch S, Hardt D, Fackelmayer FO, Hesch RD, Knippers R. DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res. 2001;61:1659-65.

Fatouros IG, Destouni A, Margonis K, Jamurtas AZ, Vrettou C, Kouretas D, Mastorakos G, Mitrakou A, Taxildaris K, Kanavakis E, Papassotiriou I. Cell-free plasma DNA as a novel marker of aseptic inflammation severity related to exercise overtraining. Clin Chem. 2006;52:1820-1824.

Zhong XY, Hahn S, Kiefer V, Holzgreve W. Is the quantity of circulatory cell-free DNA in human plasma and serum samples associated with gender, age and frequency of blood donations?. Ann Hematol. 2006;86:139-143.

Steinman CR. Free DNA in serum and plasma from normal adults. J Clin Invest. 1975;56:512-515.

Trejo-Becerril C, Oñate-Ocaña LF, Taja-Chayeb L, Vanoye-Carlo A, Cetina L, Dueñas-Gonzalez A. Serum nucleosomes during neoadjuvant chemotherapy in patients with cervical cancer. Predictive and prognostic significance. BMC Cancer. 2005;5:65.

Dong SM, Pai PI, Rha SH, Hildesheim A, Kurman RJ, Schwartz PE, Mortel R, McGowan L, Greenberg MD, Barnes WA, Sidransky D. Detection and quantitation of human Papillomavirus DNA in the plasma of patients with cervical carcinoma. Cancer Epidemiol Biomarkers Prev. 2002;11:3-6.

Pornthanakasem W, Shotelersuk K, Termrungruanglert W, Voravud N, Niruthisard S, Mutirangura A. Human papillomavirus DNA in plasma of patients with cervical. Cancer. BMC Cancer. 2001;1:2.

Rogachev VA, Likhacheva A, Vratskikh O, Mechetina LV, Sebeleva TE, Bogachev SS, Yakubov LA, Shurdov MA. Qualitative and quantitative characteristics of the extracellular DNA delivered to the nucleus of a living cell. Cancer Cell Int. 2006;6-23.

Wu TL, Zhang D, Chia JH, Tsao KC, Sun CF, Wu JT. Cell-free DNA: measurement in various carcinomas and establishment of normal reference range. Clin Chim Acta. 2002;321:77–87.

Sozzi G, Conte D, Mariano L, Lo Vullo S, Roz L, Lombardo C, Pierotti MA, Tavecchio L. Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up f lung cancer patients. Cancer Res. 2001;61:4675-4678.

Anker P, Mulcahy H, Chen XQ, Stroun M. Detection of circulating tumor DNA in the blood (plasma/serum) of cancer patients. Cancer Metastasis Rev. 1999;18:65–73.

Umetani N, Hiramatsu S, Hoon DS. Higher amount of free circulating DNA in serum than in plasma is not mainly caused by contamined extraneus DNA during separation. Ann NY Acad Sci. 2006;1075:299-307.

Umetani N, Kim J, Hiramatsu S, Reber HA, Hines OJ, Bilchik AJ, Hoon DSB. Increased integrity of free circulating DNA in sera of patients with colorectal or periampullary cancer: Direct quantitative PCR for ALU repeats. Clin Chem. 2006;52:1062-1069.

Lui YY, Chik KW, Chiu RW, Ho CY, Lam CW, Lo YM. Predominant hematopoietic origin of cf-DNA in plasma and serum after gender-mismatched bone marrow transplantation. Clin Chem. 2002;48:421–427.

license

How to Cite

Amaya Guio, J., Aristizabal Gutiérrez, F. A., Briceño Balcázar, I., Castillo Zamora, M., García Robayo, D. A., & Salazar Jordan, H. D. (2009). Quantification of free DNA in blood plasma of healthy volunteers in Bogotá population. NOVA Biomedical Sciences Journal, 7(12), 136-142. https://doi.org/10.22490/24629448.427

Download Citation

Almétricas

Metrics

Metrics Loading ...

Declaración de privacidad.

Quantification of free DNA in blood plasma of healthy volunteers in Bogotá population

How to Cite