Determinación de la asociación entre Chlamydia trachomatis y Neisseria gonorrhoeae, en muestras de orina mediante técnicas debiología molecular

Gabriela Coy BSC; Olga Lucia Ostos Ortiz MSC; Janeth Pinzón BSC; Ruth Mélida Sánchez, MSC

doi:10.22490/24629448.4

Vol. 2 No. 2 (2004)

Published 2004-12-15

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Article (before OJS)

Determine the Association Betweenandthrough Molecular Biology Techniques in Urinesamples

DOI: https://doi.org/10.22490/24629448.4

Gabriela Coy BSC Facultad de Ciencias de la Salud, Universidad Colegio Mayor de Cundinamarca.

Olga Lucia Ostos Ortiz MSC Facultad de Ciencias de la Salud, Universidad Colegio Mayor de Cundinamarca.

Janeth Pinzón BSC Laboratorio de Biología Molecular de la Secretaria de Salud de Bogotá.

Ruth Mélida Sánchez, MSC Facultad de Ciencias de la Salud, Universidad Colegio Mayor de Cundinamarca,

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Abstract
References

The following study evaluated the association between Chlamydia Trachomatis (CT) and Neisseriae Gonorrhoeae (NG) using COBAS AMPLICOR CT/NG MOLECULAR SYSTEM technique in 35 women urine samples with a 23.9 year old average. 20 samples were positive to CT, showing a prevalence of 5.63%. Although, epidemiological studies have reported a relationship between Chlamydia Trachomatis infection and the ones caused by others microorganisms like Neisseriae Gonorrhoeae; this study didn’t find any association. Factors like kind of sample and the presence of inhibitors in urine, diminished sensibility to detect Neisseriae Gonorrhoeae.

keywords: Chlamydia Trachomatis, association, Neisseriae Gonorrhoeae, PCR, sensibility, urine.

Todar K. The pathogenic Neisseria. University of Wisconsin- Madison Department of Bacteriology; 2002.

Merz AJ. Interactions of pathogenic Neisseria with epithelial cell membranes. Annu rev cell dev biol 2000; (16): 423-57.

Kanoh Y. The infections by Chlamydia trachomatis and Neisseria gonorrhoeae Rinsho Byori 2002; 123 (Suppl Nov):57-61.

Wiesenfeld HC. Bacterial vaginosis is a strong predictor of Chlamydia trachomatis and Neisseria gonorrhoeae infection. Clin Infecto Dis 2003; 36(5): 663-8.

Knox J. Evaluation of self-colected samples in contrast to practitioner-collected samples for detection of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis PCR among Women living in remote areas. Sex transm dis 2002; 29(11): 647.

Koumans EH. Comparison of methodos for detection of Chlamydia trachomatis and Neisseria gonorrhoeae using commercially available nucleic acid amplification test and liquid pap smear medium. Journal of clinical microbiology 2003; 41(4):1507-11.

Bachmann LH, Prevalence and factors associated with gonorrhoea and chamydial infection in at-risk females presenting to an urban emergency department. Sex Transm dis 2003; 30(4): 335-9.

Castillo MC. Purification and characterization of lactamase from Neisseria gonorrhoeae from clinical samples Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs and male urethral swabs. Revista latinoamericana de microbiologia 2001; 43(2):70-5.

Birch NC. Evaluation of urine specimen integrity in a public health STD screening program. Am J Pathol 2003; 119(4):516-21.

Ministerio de salud pública. Informe epidemiológico Nacional INS: 2001; Nov 30; 6(22).

Carroll K. Evaluation of the ABBOTT LCx ligase chain reaction assay for detection of Chlamydia trachomatics and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted diseases clinic population. Journal of clinical microbiology 1998; 36(6):1630-3.

Farrell D. Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR. Journal of clinical microbiology 1999; 37(2): 386-90.

Hardick J. Surveillance of Chlamydia trachomatis and Neisseria gonorrhoeae infections in women in detection in Baltimore, Maryland. Sex Transm dis 2003; 30(1): 64-70.

Taffa N. Prevalence of gonococcal and chlamydial infections and sexual risk behaviour among youth in Addis Adaba, Ethiopia. Sex transm dis 2002; 29(12): 828-33.

Palmer HM. Evaluation of the specificities of five DNA amplification methods for the detection of Neisseria gonorrhoeae Journal of Clinical Mycrobiology 2003; 41(2): 835-7.

Schachter J. Which test is best for Chlamydia? Curr Opin Infect Dis 1999; 12;41-5.

Van der pol B, et al. Multicenter evaluation of the BD ProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs and male urethral swabs. Journal of clinical microbiology 2001; 39(3): 1008-16.

Newman SB, et al. Female prisioners preferences of collection methods for testing for Chlamydia trachomatis and Neisseria gonorrhoeae infection. Sex Transm dis 2003; 30(4): 306-9.

Van doornum GJ. Comparison between the LCx probe system and the COBAS AMPLICOR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Infections in patients attending a clinic for treatment of sexually transmitted diseases in amsterdam the Netherlands. Journal of clinical microbiology 2001; 39(3): 829-35.

Makepeace B, et al. Interactions of Neisseria gonorrhoeae with Mature Human Macrophage Opacity Proteins Influence Production of Proinflammatory Cytokines. Infection and immunity 2001; 69(3): 1909-13.

Massari P. The role of porins in neisserial pathogenesis and immunity. Trends Microbiol 2003; 11(2):87-93.

Mazzone M, et al. Whole-genome organization and functional properties of miniature DNA insertion sequences conserved in pathogenic Neisseria. Gene 2001; 278(1-2): 211-22.

Mcknew DL, et al. Porin variation among clinical isolates of Neisseria gonorrhoeae over a 10 year period, as determinated by variable region typing. J Infection disease 2003; 187(8): 1213-22.

Rahman M, et al. Etiology of sexually transmitted infections among street based female sex workers in Dhaka Bangladesh. Journal of microbiology 2000; 38(3): 1244-6.

Pujol C, et al. Do pathogenic Neisseria need several ways to the host cell cytoskeleton? Microbes and infection 2000; 2(7) 821-7

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Coy BSC, G., Ostos Ortiz MSC, O. L., Pinzón BSC, J., & Sánchez, MSC, R. M. (2004). Determine the Association Betweenandthrough Molecular Biology Techniques in Urinesamples. NOVA Biomedical Sciences Journal, 2(2), 28-32. https://doi.org/10.22490/24629448.4

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Determine the Association Betweenandthrough Molecular Biology Techniques in Urinesamples

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