Purificación rápida de inmunoglobulina M a partir de suero de cachama blanca (piaractus brachypomus) y preparación de antisuero policlonal en conejo

Francy J. Montoya; Jorge E. Almanza M. MSC; Sandra Parada; Pedro René Eslava; Alfredo Arias; Rubén Darío Toro; Joyce Andrea Rojas

doi:10.22490/24629448.385

Vol. 5 No. 8 (2007)

Published

2007-12-15

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Rapid Purification Immunoglobulin M From Serum of White Cachama () and Preparation of a Rabbit Polyclonal Antiserum.

DOI: https://doi.org/10.22490/24629448.385

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Research Article (before OJS)

Francy J. Montoya Corpoica, Ceisa, Bogotá.

Jorge E. Almanza M. MSC Programa Estratégico de Investigación Biomédica, Universidad Antonio Nariño, Bogotá.

Sandra Parada Instituto de Acuicultura de la Universidad de los Llanos Orientales, IALL.

Pedro René Eslava

Alfredo Arias Instituto de Acuicultura de la Universidad de los Llanos Orientales, IALL.

Rubén Darío Toro Corpoica, Ceisa, Bogotá.

Joyce Andrea Rojas

In spite of the recognized rusticity of the cachama, the increasing tendency towards the intensification of the production systems of the species, begins to favor the increase of sanitary problems, which will have to be characterized in order to be able to establish methods of diagnosis and rational plans of prevention. Since the knowledge of ictiopathology of native species is incipient, among other things because of the little availability of information on basic aspects of histology, embryology, physiology, anatomopathology, immunology and epidemiology, the establishment of investigation projects that allow to know such aspects in order to support the production of these species technically becomes necessary. With this work begins the acquisition of knowledge about the basic immunologic aspects of the White Cachama (Piaractus brachypomus).

For this end, gamma-globulins were purified by means of the elution starting from agarosa gels. The presence of two possible attachés of IgM was confirmed. One of them seems to correspond to form monomerics and the dependability of this purification method allows us to obtain small quantities of protein without the presence of pollutants. The reactivity of the obtained antiserum only reacted against the fraction of the gamma-globulins, what was proven by means of the inmunoelectroforesis technique with the serums of White Cachama. Also, they obtained two bands with SDS - PAGE under conditions without reduction; the first one with an approximate weight of 80 kDa and the second one about 70 kDa. Under reduced conditions, two bands were obtained, the first one with an approximate weight of 90 kDa and the second of 70 kDa.

cachama, electrophoresis, gamma-globulines, ictiopathology, immunization, immunoglobulins.

Díaz FJ, López R A. El cultivo de cachama. INPA;1990:23.

Estevez M. La cachama: Colossoma macropomum Cultivo en estanques. Ministerio de Agricultura, Instituto Nacional de Recursos Naturales Renovables y del Medio Ambiente (INDERENA). Colombia. 1990.

Martínez H, González F. La Cadena de la piscicultura en Colombia: Una Mirada Global de su Estructura y Dinámica. En www.agrocadenas.gov.co Documento de Trabajo No 72. 2005.

Ingram G.A. Complement-fixation test. En: Techniques in fish immunology. FITC-1. J.S. Stolen, T.C. Fletcher, D.P. Anderson, B.S. Roberson y W.B. Muiswinkel. SOS Publications. Fair Haven, New Jersey. USA. 1990; 25-44.

Rodríguez G, González O, Mariño C. Manual de técnicas en microbiología. ICA. Documento de trabajo No. 18. Código 10- 6-018-78. Bogotá (Colombia). 1978:506.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680-685.

Aguillon JC. Anticuerpos policlonales antiinmunoglobulinas de salmón: Herramientas potenciales para diagnóstico. Arch Med Vet. 1997;29:133-139.

Palenzuela O, Sitja-Bobadilla A, Alvarez-Pellitero P. Isolation and partial characterization of serum immunoglobulins from sea bass (Dicentrarchu labrax L.) and gilhead sea bream (Spaurus aurata L.). Fish Shellfish Immunology. 1996;6:81–94.

Bourmaud C, Romestand B, Bouix G. Isolation and partial characterization of IgM-like seabass (Dicentrarchus labrax L. 1758) immunoglobulins. Aquaculture. Science 1995;132:53–58.

Verjan N, Bobadilla N. Estudio electroforético de las proteínas séricas de la cachama blanca (Piaractus brachypomus) cultivada en el departamento del Meta. Tesis Universidad de los Llanos. 1998;190.

Coeurdacier J, Pepin J, Fauvel C, Legall P, Bourmaud A, Romestand B. Alterations in total protein, IgM and specific antibody activity of male and female sea bass (Dicentrarchu labrax L., 1758) sera following injection with killed Vibrio anguillarum. Fish & Shellfish Immunology. 1997;7:151–160.

Ellis A. Ontogeny of the immune response in Salmo salar. Histogenesis of the lymphoid organs and appearance of membrane immunoglobulin and mixed leukocyte reactivity. In: Developmental Immunology. Elsevier/North – Holland Biomedical Press Amsterdam, 1977;225-231.

Rombout JH, Taverne N, van de Kamp M, Taverne-Thiele AJ. Differences in mucus and serum immunoglobulin of carp (Cyprinus carpio L.). Dev Comp Immunol. 1993;17:309-317.

Koumans-van Diepen JC, Egberts E, Peixoto BR, Taverne N, Rombout JH B cell and immunoglobulin heterogeneity in carp (Cyprinus carpio L.); an immuno(cyto)chemical study. Dev Comp Immunol. 1995;19:97-108.

Montoya, F. J., Almanza M. MSC, J. E., Parada, S., Eslava, P. R., Arias, A., Toro, R. D., & Rojas, J. A. (2007). Rapid Purification Immunoglobulin M From Serum of White Cachama () and Preparation of a Rabbit Polyclonal Antiserum. NOVA Biomedical Sciences Journal, 5(8), 161-167. https://doi.org/10.22490/24629448.385

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Rapid Purification Immunoglobulin M From Serum of White Cachama () and Preparation of a Rabbit Polyclonal Antiserum.

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