Aislamiento de los microorganismos cultivables de la rizosfera de Ornithogalum umbellatum y evaluación del posible efecto biocontrolador en dos patógenos del suelo

Solange Benítez; Johanna Bentley; Paula Bustamante; Ligia Consuelo Sánchez; Lucía Corrales

doi:10.22490/24629448.383

Vol. 5 No. 8 (2007)

Published

2007-12-15

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Isolating Culturedrhizosphere Microorganisms and Assessment of Possible Biocontrolling Effect On Two Ground Pathogens

DOI: https://doi.org/10.22490/24629448.383

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Research Article (before OJS)

Solange Benítez Universidad Colegio Mayor de Cundinamarca

Johanna Bentley Universidad Colegio Mayor de Cundinamarca

Paula Bustamante Universidad Colegio Mayor de Cundinamarca

Ligia Consuelo Sánchez Universidad Colegio Mayor de Cundinamarca

Lucía Corrales Universidad Colegio Mayor de Cundinamarca

The microorganisms present in the ground exert a specific action when related to a vegetal species. Ornithogalum umbellatum is a wild plant used in the country as ornamental but it is recognized in other parts of the world by the chemical components that the bulb has and by the application of these substances for the alternative treatment of different diseases. In effect, this particularity is the one that leads the investigators to raise the hypothesis that the existing microflore in the rhizosphere could have possible biocontrolers effects in phytopathogens of the ground and to make an evaluation of the antagonistic effect in vitro with Botrytis cinerea and Sclerotinia sclerotiorum.

The study was made taking 12 samples from rhizosphere and seeding them by duplicate in solids average, agar dextrose potatoe, agar Bengal Rose, (with and without antibiotic), agar yeast mold and agar Luria Bertani. 8 morphotypes of leavenings, 5 morphotypes of bacteria and 9 morphotypes of moulds were isolated; most of them demonstrated antagonism in vitro with the selected phytopathogens. This study allows us to conclude that in the rhizosphere of the plant O. umbellatum are microorganisms that demonstrated antagonism in vitro and that they could be used like biocontrolers of Botrytis cinerea and Sclerotinia sclerotiorum. In addition, it was observed that some of the isolated microbial morphotypes displayed a very discreet antagonism, but they caused changes in the structure and color of hyphae of the used phythopatogens fungi. It leads us to think that the concentration of the biocontroler influences the mechanisms of biocontrol.

biocontrol, Ornithogalum umbellatumrizosphere, phytophatogens, rhizosphere, Sclerotinia sclerotiorum.

AzziorI O, Braemer R, Paris M. Glucosylflavones in the genus Ornithogalum. Biochem Syst Ecol 1989;17: 449-450.

Kuroda M, Mimaki Y, Yokosuka A, Hasegawa F, Sashida Y. Cholestane glycosides from the bulbs of Ornithogalum thyrsoides and their cytotoxic activity against HL-60 leukemia cells. J Nat Prod 2002; 65:1417 1423.

Mimaki Y, Kuroda M, Sashida Y, Hirano T, Oka K, Dobashi A. Three Novel Rearranged Cholestane Glycosides from Ornithogalum saundersiae Bulbs and Their Cytostatic Activities on Leukemia HL-60 and MOLT-4 Cells. Tetrahedron Lett 1996;37:1245-1248.

Hirano T, Oka K, Mimaki Y, Kuroda M, Sashida Y. Potent growth inhibitory activity of a novel ornlthogalum cholestane glycoside on human cells: induction of apoptosis in promyelocytic leukemia HL-60 cells. Life Sciences. 1996; 58:739-798.

Fernández O, Vega L. Microorganismos antagonistas para el control fitosanitario. Manejo integrado de plagas 2001; 62:96-100.

Bautista G. Determinación de la asociación y antagonismo contra R. solani de aislamientos nativos de Pseudomonas fluoresens en un cultivo de papa Solanum phuroja en condiciones de invernadero. Universidad Nacional. Maestría de ínter facultades en Microbiología. 2000.

Carruthers F, Connere A, Mahonty H. Identification of genetic locus in P.aureofacions involved I fungal inhibition. Applied. Env Microbiol. 1994;60:71-75.

Uribe D, Ortiz E, Portillo M, Bautista G, Ceron J. Diversidad de pseudomonas fluorescentes en cultivos de papa de la región cundí boyacense y su actividad antagonista in vitro sobre Rhizoctonia solani. Rev. Colombiana de Biotecnología. 1999; 6: Nº 1.

Mead G, Hudson W, Hinton M. Microbiological survey of five poultry processing plants in the UK. Br Poult Sci. 1993;34:497-503.

Cundom M, Mazza S, Mazzanti M, Gutierrez S, Coutinho M. Actividad antagónica in vitro de Hongos Saprofitos sobre S. sclerotiorum. Cátedra de Fitopatología, Facultad de ciencias agrarias, UNNE Argentina. 1998.

Molina G, Forero de la R C, Torres E.. Incidencia de Infecciones Quiescientes de Moho Gris, causado por Botrytis cinerea, en flores, en frutos de mora de Castilla Rubus glaucus. Fitopatología colombiana 2004; 28: 49-56.

Benítez, S., Bentley, J., Bustamante, P., Sánchez, L. C., & Corrales, L. (2007). Isolating Culturedrhizosphere Microorganisms and Assessment of Possible Biocontrolling Effect On Two Ground Pathogens. NOVA Biomedical Sciences Journal, 5(8), 147-153. https://doi.org/10.22490/24629448.383

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Isolating Culturedrhizosphere Microorganisms and Assessment of Possible Biocontrolling Effect On Two Ground Pathogens

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