Determinación de valores de referencia para la microtécnica de fagocitosis y muerte intracelular deCándida álbicans en un grupo de población sana en Bogotá

Yolanda Cifuentes MD; Liliana Muñoz Molina MSC; Jeannette Navarrete MSC; Gladys Pinilla MSC; Ariel Iván Ruiz Parra MD MSC

doi:10.22490/24629448.370

Vol. 5 No. 7 (2007)

Published

2007-06-15

PDF (Spanish)

How to Cite

Cifuentes MD, Y., Muñoz Molina MSC, L., Navarrete MSC, J., Pinilla MSC, G., & Ruiz Parra MD MSC, A. I. (2007). Determination of Reference Values for the Microtechnique of Phagocytosis and Intracellelar Death of Candida Albicans in a Group of Healthy Population in Bogota. NOVA Biomedical Sciences Journal, 5(7), 31-37. https://doi.org/10.22490/24629448.370

Download Citation

Metrics

Metrics Loading ...

Determination of Reference Values for the Microtechnique of Phagocytosis and Intracellelar Death of Candida Albicans in a Group of Healthy Population in Bogota

DOI: https://doi.org/10.22490/24629448.370

Section

Article (before OJS)

Yolanda Cifuentes MD Universidad Nacional de Colombia

Liliana Muñoz Molina MSC Universidad Colegio Mayor de Cundinamarca Facultad de Ciencias de la Salud Programa de Bacteriología y Laboratorio Clínico

Jeannette Navarrete MSC Universidad Colegio Mayor de Cundinamarca Facultad de Ciencias de la Salud Programa de Bacteriología y Laboratorio Clínico

Gladys Pinilla MSC Universidad Colegio Mayor de Cundinamarca Facultad de Ciencias de la Salud Programa de Bacteriología y Laboratorio Clínico

Ariel Iván Ruiz Parra MD MSC Universidad Nacional de Colombia

The function of the phagocytic cells is an efficient mechanism for non-specific protection against infectious agents and elimination of dead or senile cells. Cells of the innate immunity, such as neutrophils and macrophages, are responsible for this process. Neutrophils are particularly effective at the initial phases of infections caused by Gram negative and Gram positive bacteria.

Macrophages participate in late stages of inflammation phagocyting bacteria and cellular debris. The techniques for evaluation of the phagocytic capacity are laborious, require large blood samples and need excessive manipulation of the cells. The micro technique of intracellular death of Candida albicans is a simple and fast technique, which requires a very little blood sample. This technique was used in 56 samples of healthy people to determine reference values of the phagocytic index and the percentage of intracellular death, which are indicators of neutrophils´ phagocytic capacity.

The phagocitic index was 2.70 +/- 0.43 (mean +/- SD), range 2.03 - 3.92, and the percentage of intracellular death was 31.31 +/- 5.10 (mean +/- SD), range 21 - 39. There were no differences by age groups, gender and number of leucocytes or percentage of neutrophils.

Cándida albicans, innate immunity, neutrophils, opsoninas, phagocytosis.

Kapetanovic R, Cavaillon J. Early events in innate immunity in recognition of microbial pathogens. Expert Opin Biol Ther. 2007;7:907-918.

Johnston R. Function and cell biologyof neutrophils and mononuclear phagocytes in the newborn infant. Vaccine. 1998;16:1363-1368.

Rojas O, Arce P. Fagocitosis:mecanismos y consecuencias. Bioquímica. 2004;29:18-31.

Molina R, Pérez L, Sánchez L. Evaluación de la función opsono fagocítica de los neutrófilos en pacientes infectados por el VIH. Rev Cubana Hematol Inmunol Hemoter. 2002;18:1-4.

Enrique B. El sistema inmunológico: Reconocimiento de lo propio y lo no propio. Rev. SEAIC Alergología e Inmunología Clínica, 2005: p. 24-27.

Muniz F, Silva-Filho. da L, Cardosa A, Tosta C. Novel Microtechnique for Assessment of Postnatal Maturation of the Phagocytic Function of Neutrophils and Monocytes. Clin Diag Lab Immunol. 2003;10:1096 1102.

Oseas R, Leher R. A micromethod for measuring neutrophil candidacidal activity in neonats. Pediat Res 1978;12:828-829.

Berrio M. El Hemograma. Medellín, Colombia: Corporación para Investigaciones Biológicas; 2006.

Wardowska A, Dzierzbicka K, Trzonkowski P, Mysliwski A. The biological activity of new tuftsin derivatives—induction of phagocytosis. Acta Pol Pharm. 2006;63:378-81

Unanue E. Interactions of pathogens with the innate and adaptive immune system. In: Cossart P, Boquet P, Normark S, Rappuoli R, eds. Cellular microbiology. Washington DC: ASM Press. 2000;291-311.

Kim S, Gershov D, Ma X, Brot N, Elkon K. Opsonization of apoptotic cells and its effect on macrophage and T cell immune responses. Ann N Y Acad Sci. 2003;987:68-78.

Van Eeden S, Klut M, Walter A, Hogg J. The use of flow citometry to measure neutrophil function. J. Immunol Methods. 1999;232:23-43

Egido JM, Viñuelas J. Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans. Rev Soc Bras Med Trop. 1997;30:51-58.

Vega B, García J, Hernandez F. Immunoglobulin Fc receptors in clinical strains of Staphylococcus aureus do not confer resistance to Phagocytosis in an in vitro assay. Rev Inst Med Trop S Paulo 1999;41: 45-49.

Download Citation

Published

How to Cite

Determination of Reference Values for the Microtechnique of Phagocytosis and Intracellelar Death of Candida Albicans in a Group of Healthy Population in Bogota

Declaración de privacidad.

Keywords