Determinación de valores de referencia para la microtécnica de fagocitosis y muerte intracelular deCándida álbicans en un grupo de población sana en Bogotá

Yolanda Cifuentes MD; Liliana Muñoz Molina MSC; Jeannette Navarrete MSC; Gladys Pinilla MSC; Ariel Iván Ruiz Parra MD MSC

doi:10.22490/24629448.370

Vol. 5 No. 7 (2007)

Published 2007-06-15

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Determination of Reference Values for the Microtechnique of Phagocytosis and Intracellelar Death of Candida Albicans in a Group of Healthy Population in Bogota

DOI: https://doi.org/10.22490/24629448.370

Yolanda Cifuentes MD Universidad Nacional de Colombia

Liliana Muñoz Molina MSC Universidad Colegio Mayor de Cundinamarca Facultad de Ciencias de la Salud Programa de Bacteriología y Laboratorio Clínico

Jeannette Navarrete MSC Universidad Colegio Mayor de Cundinamarca Facultad de Ciencias de la Salud Programa de Bacteriología y Laboratorio Clínico

Gladys Pinilla MSC Universidad Colegio Mayor de Cundinamarca Facultad de Ciencias de la Salud Programa de Bacteriología y Laboratorio Clínico

Ariel Iván Ruiz Parra MD MSC Universidad Nacional de Colombia

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Abstract
References

The function of the phagocytic cells is an efficient mechanism for non-specific protection against infectious agents and elimination of dead or senile cells. Cells of the innate immunity, such as neutrophils and macrophages, are responsible for this process. Neutrophils are particularly effective at the initial phases of infections caused by Gram negative and Gram positive bacteria.

Macrophages participate in late stages of inflammation phagocyting bacteria and cellular debris. The techniques for evaluation of the phagocytic capacity are laborious, require large blood samples and need excessive manipulation of the cells. The micro technique of intracellular death of Candida albicans is a simple and fast technique, which requires a very little blood sample. This technique was used in 56 samples of healthy people to determine reference values of the phagocytic index and the percentage of intracellular death, which are indicators of neutrophils´ phagocytic capacity.

The phagocitic index was 2.70 +/- 0.43 (mean +/- SD), range 2.03 - 3.92, and the percentage of intracellular death was 31.31 +/- 5.10 (mean +/- SD), range 21 - 39. There were no differences by age groups, gender and number of leucocytes or percentage of neutrophils.

keywords: Cándida albicans, innate immunity, neutrophils, opsoninas, phagocytosis.

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Cifuentes MD, Y., Muñoz Molina MSC, L., Navarrete MSC, J., Pinilla MSC, G., & Ruiz Parra MD MSC, A. I. (2007). Determination of Reference Values for the Microtechnique of Phagocytosis and Intracellelar Death of Candida Albicans in a Group of Healthy Population in Bogota. NOVA Biomedical Sciences Journal, 5(7), 31-37. https://doi.org/10.22490/24629448.370

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Determination of Reference Values for the Microtechnique of Phagocytosis and Intracellelar Death of Candida Albicans in a Group of Healthy Population in Bogota

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