Perspectivas en investigación: : células HEp-2 infectadas con EB’s de Chlamydia trachomatis serovar 2 (VR-902B)

Natalia  Castellanos Hernández; Yessica Marcela Castañeda Franco; Paola Andrea Caro Burgos; Ruth Mélida Sánchez Mora

doi:10.22490/24629448.3696

Vol. 18 No. 33 (2020)

Published 2020-02-10

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Chlamydia trachomatis:

DOI: https://doi.org/10.22490/24629448.3696

Natalia Castellanos Hernández

Yessica Marcela Castañeda Franco

Paola Andrea Paola Andrea

Ruth Mélida Sánchez Mora

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Abstract
References

Chlamydia trachomatis (C. Trachomatis) is a Gram negative unmoving bacterium, characterized by being an obligate intracellular microorganism and having a reproductive cycle in which a metabolically inactive extracellular infectious form (elementary body - EB's) can be distinguished from an intracellular active and non-infectious form (reticulated body - RB's). C trachomatis is characterized by causing infection in humans, is related to sexually transmitted diseases and eye infections, so it can lead to sequelae of interest if timely treatment is not given. The objective of this study was to optimize the infection model of C. trachomatis in HEp-2 cells with elementary bodies (EB’s) of C. trachomatis serovar L2. Initially, the conditions for the adequate growth of HEp-2 cells were established in time and with a confluence of 90%, to continue with the optimization of an infection protocol. The infection was confirmed from the staining with Giemsa allowing to evaluate morphological characteristics of both uninfected and infected HEp-2 cells and also of the elementary bodies of C. trachomatis. Finally, the infection was corroborated with the direct immunofluorescence technique, that detects the C. trachomatis MOMP membrane protein. After the tests were performed, the presence of elementary bodies nearby and within the cellular cytoplasm was evidenced, as well as vacuolated cells and cellular damage caused by the infection.

keywords: C. trachomatis, cultivo celular, asintomático, infección, persistencia, Giemsa, inmunofluorescencia, HEp-2, LGV

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Castellanos Hernández, N. ., Castañeda Franco, Y. M., Paola Andrea, P. A., & Sánchez Mora, R. M. (2020). Chlamydia trachomatis: : HEp-2 Cells Infected with EB’s from Chlamydia trachomatis serovar 2 (VR-902B): Research Perspectives. NOVA Biomedical Sciences Journal, 18(33). https://doi.org/10.22490/24629448.3696

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