Implementación y evaluación de dos métodos deconservación y generación de la base de datos del banco de cepas y genes del Instituto de Biotecnología de la Universidad Nacional de Colombia (IBUN)

Sandra Liliana Parra Huertas; María Marcela Pérez Casas; Mauricio Bernal Morales; Zulma Suárez Moreno; Dolly Montoya Castaño

doi:10.22490/24629448.346

Vol. 4 No. 5 (2006)

Published 2006-06-15

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Implementation and Evaluation of Two Conservation and Generation Methods of the Data Base of the Vine-Stocks and Genes Bank of the Institute of Biotecnology of the National University of Colombia (Ibun)

DOI: https://doi.org/10.22490/24629448.346

Sandra Liliana Parra Huertas Universidad Colegio Mayor de Cundinamarca

María Marcela Pérez Casas Universidad Colegio Mayor de Cundinamarca

Mauricio Bernal Morales Instituto de Biotecnología de la Universidad Nacional2, Bogotá, Colombia.

Zulma Suárez Moreno Instituto de Biotecnología de la Universidad Nacional2, Bogotá, Colombia.

Dolly Montoya Castaño Instituto de Biotecnología de la Universidad Nacional

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Abstract
References

76 strains of the family Enterobacteriaceae and Pseudomonadaceae were conserved by filter paper semistock method and 68 strains by Cryopreservation stock method in the bank of strains and genes of Biotechnology Institute of the Universidad Nacional de Colombia (IBUN). The effectiveness of methods was tested for the viability at 24 hours after preservation and was calculated the recovery percentage. Thus in conclusion, the preservation in paper filter and cryopreservation are suitable semistock and Stock methods, respectively; because 73.7% of family Enterobacteriaceae microorganisms and 82.3% of family Pseudomonadaceae microorganisms, presented a high recovery percentage greater 50%. In addition, was implemented a strain bank data base made in software Access ®, in Biotechnology Institute and Universidad Nacional de Colombia Web page www.ibun.unal.edu.co/instituto/cepario . It is available for general public.

keywords: cryopreservation, viability, semistock culture, stock culture, database

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Parra Huertas, S. L., Pérez Casas, M. M., Bernal Morales, M., Suárez Moreno, Z., & Montoya Castaño, D. (2006). Implementation and Evaluation of Two Conservation and Generation Methods of the Data Base of the Vine-Stocks and Genes Bank of the Institute of Biotecnology of the National University of Colombia (Ibun). NOVA Biomedical Sciences Journal, 4(5), 39-49. https://doi.org/10.22490/24629448.346

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Implementation and Evaluation of Two Conservation and Generation Methods of the Data Base of the Vine-Stocks and Genes Bank of the Institute of Biotecnology of the National University of Colombia (Ibun)

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