Mycobacterium tuberculosisESAT-6 antigen immunogenicity in Owl Monkeys

Rosalba Alfonso; Paola Barato; Martha Calderon; Diana Giraldo; Martha Pinto; Carlos Parra-López; Manuel A. Patarroyo.

doi:10.22490/24629448.344

Vol. 4 No. 5 (2006)

Published 2006-06-15

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Mycobacterium tuberculosisESAT-6 antigen immunogenicity in Owl Monkeys

DOI: https://doi.org/10.22490/24629448.344

Rosalba Alfonso Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

Paola Barato Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

Martha Calderon Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

Diana Giraldo Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

Martha Pinto Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

Carlos Parra-López Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

Manuel A. Patarroyo. Molecular Biology Department, Fundación Instituto de Inmunología de Colombia Universidad Nacional de Colombia

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Abstract
References

Several ESAT-6-based vaccines have been widely studied in different animal models, presenting potent ability to induce both cellular and humoral responses. As ESAT-6 is a well-characterized mycobacterial antigen, its capacity to induce immune responses in a nonhuman primate model has been evaluated. The immunization of recombinant ESAT-6 (rESAT-6) in Aotus nancymaae monkeys has elicited a strong cellular response, not just to rESAT-6, but also to the native protein present in Mycobacterium tuberculosis culture filtrate proteins measured as [3H]thymidine incorporation in lymphocyte proliferation assays. High humoral response was also observed, having antibody titers of 1:12,800 directed towards rESAT-6. The protein.s multi-epitope nature was further demonstrated since several peptide sequences were specifically recognized at both cellular and humoral level. The high immunogenicity observed, as well as the relatively high characterization of the Aotus. immune system at molecular level, are two advantage to propose Aotus nancymaae as animal model for studying M. tuberculosis infection; however, our results reveal an animal-to-animal variation in response to vaccination, this could be a disadvantage

keywords: Owl monkey, animal model, Mycobacterium tuberculosis ESAT-6, immune response.

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Alfonso, R., Barato, P., Calderon, M., Giraldo, D., Pinto, M., Parra-López, C., & Patarroyo., M. A. (2006). Mycobacterium tuberculosisESAT-6 antigen immunogenicity in Owl Monkeys. NOVA Biomedical Sciences Journal, 4(5), 14-26. https://doi.org/10.22490/24629448.344

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Mycobacterium tuberculosisESAT-6 antigen immunogenicity in Owl Monkeys

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