Caracterización Molecular de Cepas Colombianas de . a Través del Rflp-is200

Elizabeth Vásquez; Salim Máttar; Néstor Mossos; Darío Mogollón; Raúl Alberto Poutou Piñales BQ MSC

doi:10.22490/24629448.17

Vol. 3 No. 3 (2005)

Published

2005-06-15

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Vásquez, E., Máttar, S., Mossos, N., Mogollón, D., & Poutou Piñales BQ MSC, R. A. (2005). Molecular Characterization of Colombian Stumps of . Through the Rflp-is200. NOVA Biomedical Sciences Journal, 3(3), 37-45. https://doi.org/10.22490/24629448.17

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Molecular Characterization of Colombian Stumps of . Through the Rflp-is200

DOI: https://doi.org/10.22490/24629448.17

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Article (before OJS)

Elizabeth Vásquez

Salim Máttar

Néstor Mossos Laboratorio de Diagnostico

Darío Mogollón Laboratorio de Diagnostico

Raúl Alberto Poutou Piñales BQ MSC Pontificia Universidad Javeriana, Bogotá, D.C.

Molecular techniques, used for the genomic DNA analysis, as IS200 RFLP has shown to be efficient to solve phylogenetic and epidemiologic relationship between Salmonella spp., strains, isolated from poultry and human. Present study had the aim to determine whether the IS200 sequence was present in the Salmonella spp., strains isolated from both origins, to establish a possible transmition chain between both. Phylogenetic analysis of Salmonella strains showed 13 IS200 profiles that exhibit between 1 and 11 copies of the insertion sequence, located in a range size between 13 and 1.7kb. This work allowed to conclude that IS200 is an efficient, sensible and specific molecular marker, useful to epidemiological studies because it capacity of discriminating between different origin strains and showed the existence of a relationship between this strains.

The results obtained in this work showed the necessity of implementing sanitary rules to minimize the propagation of Salmonella spp., in the poultry production.

RFLP, Salmonella spp., IS200, dendogram.

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Molecular Characterization of Colombian Stumps of . Through the Rflp-is200

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