Efecto de la composición del medio de cultivo sobre la capacidad antagónica de los basidiomicetos comerciales contra Fusarium spp. y Alternaria alternata

Jeny Paola Pérez Holguína; Juan Carlos Quitian; Alexander Puentes Santos; Astrid Maribel Aguilera Becerra; Ingrid Rocio  Fonseca-Guerra

doi:10.22490/24629448.10559

Vol. 24 No. 46 (2026): 1

Published 2025-11-11

license

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Research Article (before OJS)

Effect of Culture Medium Composition on the Antagonistic Capacity of Commercial Basidiomycetes against Fusarium spp. and Alternaria alternata

DOI: https://doi.org/10.22490/24629448.10559

Jeny Paola Pérez Holguína Universidad de Boyacá

Juan Carlos Quitian Universidad de Boyacá

Alexander Puentes Santos Universidad de Boyacá

Astrid Maribel Aguilera Becerra Universidad de Boyacá

Ingrid Rocio Fonseca-Guerra Universidad de Boyacá

PDF (Spanish)

Abstract
References

Objective. To evaluate the antagonistic capacity of basidiomycetes against relevant phytopathogens in vitro, in search of strains with antagonistic capacity. Methods. In the present study, antagonism assays were conducted in eight culture media differing in carbon and nitrogen sources, mineral salts, and vitamins. Results. Qualitative and quantitative observations demonstrated antibiosis by H. erinaceus and T. versicolor, which exhibited marked mycoparasitism against all phytopathogenic species tested, whereas Ganoderma showed limited in vitro antagonism. Conclusion. These findings highlight the utility of antagonism assays as a bioprospecting tool for screening and identifying basidiomycetes with controlled potential against fungal phytopathogens.

keywords: culture medium composition, antifungal activity, dual culture, antagonism, biological control, mushrooms, composición del medio de cultivo; actividad antifúngica; antagonismo en cultivo dual; control biológico; hongos

1. Agamez Ramos E, Barrera Violeth J, Oviedo Zumaqué L. Evaluación del antagonismo y multiplicación de Trichoderma sp. en sustrato de plátano en medio líquido estático. Acta Biol Colomb 14 (2009), pp. 61-70.

2. Andrade-Bustamante G, Aispuro Hernández E, Hernández-Montiel LG, Holguín Peña RJ, Rueda-Puente EO. Antisuero vs hongos fitopatógenos en el cultivo de tomate en Sonora, México. Rev Mex Cienc Agríc 10 (2019), pp. 873-884, 10.29312/remexca.v10i4.1706.

3. Baby S, Johnson AJ, Govindan B. Secondary metabolites from Ganoderma. Phytochemistry 114 (2015), pp. 66-101, 10.1016/j.phytochem.2015.03.010.

4. Bakratsas G, Polydera A, Katapodis P. Recent trends in submerged cultivation of mushrooms and their bioactive metabolites. Curr Res Biotechnol. 2021;3:185–197.

5. Bell D, Well H, Markham C. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology. 1982;72:379–382.

6. Bertrand S, Schumpp O, Bohni N, Bujard A, Azzollini A, Monod M, et al. Detection of metabolite induction in fungal co-cultures on solid media by high-throughput differential ultra-high-pressure liquid chromatography-time-of-flight mass spectrometry fingerprinting. J Chromatogr A 1292 (2013), pp. 219-228, 10.1016/j.chroma.2013.01.098.

7. Carranza CS, Barberis CL, Chiacchiera SM, Magnoli CE. Assessment of growth of Aspergillus spp. from agricultural soils in the presence of glyphosate. Rev Argent Microbiol 49 (2017), pp. 384-393, 10.1016/j.ram.2016.11.007.

8. Dudekula UT, Ram U, Qazi M, Khan N. A critical review on submerged production of mushroom bioactive metabolites. 3 Biotech. 2020;10:522.

9. Dullah S, Hazarika DJ, Parveen A, Kakoti M, Borgohain T, Gautom T, et al. Fungal interactions induce changes in hyphal morphology and enzyme production. Mycology 12 (2021), pp. 279-295, 10.1080/21501203.2021.1932627.

10. Elisashvili VI, Kachlishvili ET, Wasser SP. Carbon and nitrogen source effects on basidiomycetes exopolysaccharide production. Appl Biochem Microbiol. 2009;45(5):531–535.

11. Ezziyyani M, Requena ME, Sánchez CP, Castillo MEC. Efecto del sustrato y la temperatura en el control biológico de Phytophthora capsici en pimiento (Capsicum annuum L.). Dialnet (2005).

12. Fonseca-Guerra IR, Beltrán Pineda ME, Benavides Rozo ME. Characterization of Alternaria alternata and Alternaria scrophulariae Brown Spot in Colombian quinoa (Chenopodium quinoa). J Fungi 9 (2023), 947, 10.3390/jof9090947.

13. Fonseca-Guerra IR, Chiquillo-Pompeyo JC, Rozo MEB, Ovalle JFD. Fusarium spp. associated with Chenopodium quinoa crops in Colombia. Sci Rep 12 (2022), 10.1038/s41598-022-24908-w.

14. Gao Y, Li Y, Xie F, Zhang H, Zhang J, Xu J, et al. The liquid-fermentation formulation of Sanghuangporus sanghuang: Optimization of nutrient sources and fermentation parameters. J Fungi. 2024;10(2):120.

15. Hernández Domínguez C, Vázquez Benito JA, Vázquez Moreno F, Berdeja Arbeu R, Morales Fernández SD, Reyes López D. Abundancia y diversidad genética de Fusarium oxysporum y Trichoderma sp. en Musa AAB. Rev Mex Cienc Agríc 10 (2019), pp. 1783-1796, 10.29312/remexca.v10i8.1831.

16. Hernández-Ochoa JS, Levin LN, Hernández-Luna CE, Contreras-Cordero JF, Niño-Medina G, Chávez-Montes A, et al. Antagonistic potential of Macrolepiota sp. against Alternaria solani as causal agent of early blight disease in tomato plants. Gesunde Pflanzen 72 (2019), pp. 69-76, 10.1007/s10343-019-00484-4.

17. Hu G, Guo M, Xu J. Optimization of culture condition for ganoderic acid and polysaccharide production by Ganoderma lucidum. J Food Drug Anal. 2018;26(2):706–715.

18. Imtiaj A, Jayasinghe C, Lee GW, Lee TS. Antibacterial and antifungal activities of Stereum ostrea, an inedible wild mushroom. Mycobiology 35 (2007), pp. 210-214, 10.4489/MYCO.2007.35.4.210.

19. Jorcin G, Barneche S, Vázquez A, Cerdeiras MP. Effects of culture conditions on antimicrobial activity of Ganoderma resinaceum (Agaricomycetes) extracts. Plants 12 (2013), 10.3390/plants12122271.

20. Ling L, Feng L, Li Y, Yue R, Wang Y, Zhou Y. Endophytic fungi volatile organic compounds as crucial biocontrol agents used for controlling fruit and vegetable postharvest diseases. J Fungi 10 (2024), 332, 10.3390/jof10050332.

21. Mejía Betancourt, F. D. L. Á., & Alvarado Rodríguez, R. Y. (2016). Evaluación in vitro de hongos nativos antagonistas de Moniliophthora rereri (Cif. & Par., Evans et al.,) en el cultivo de cacao (Theobroma cacao L.) (Doctoral dissertation, Universidad Nacional Agraria).

22. Pajares JM, Gisbert JP. Helicobacter pylori: su descubrimiento e importancia en la medicina. Rev Esp Enferm Dig 98 (2006), pp. 770-785.

23. Qiu Z, Wu X, Zhang J, Huang C, Li Q. High-temperature–induced changes of extracellular metabolites of Pleurotus ostreatus promote the growth of Trichoderma asperellum. Front Microbiol. 2018;9:10.

24. Song X, Gaascht F, Schmidt-Dannert C, Salomon CE. Discovery of antifungal and biofilm preventative compounds from mycelial cultures of a unique North American Hericium sp. fungus. Molecules 25 (2020), 963, 10.3390/molecules25040963.

25. Tao Z, Dang T, Lu W, Zhu H, Zhao W, Wang T. Yeast extract: Characteristics, production, applications and future perspectives. Food Res Int. 2023;168:112742.

26. Tonial F, Maia BHLNS, Gomes-Figueiredo JA, Sobottka AM, Bertol CD, Nepel A, et al. Influence of culturing conditions on bioprospecting and the antimicrobial potential of endophytic fungi from Schinus terebinthifolius. Curr Microbiol 72 (2016), pp. 173-183, 10.1007/s00284-015-0929-0.

27. Torres López AM, Quintero Díaz JC, Atehortua Garcés L. Efecto de nutrientes sobre la producción de biomasa del hongo medicinal Ganoderma lucidum. Rev Colomb Biotecnol 13 (2011), pp. 103-109.

28. Villota-Burbano JE, Vazquez-Ochoa OY. Evaluación in vitro del extracto etanólico de hojas de borraja (Borago officinalis) contra la actividad fungistática. Agron Costarricense 45 (2021), pp. 9-27, 10.15517/rac.v45i2.47764.

29. Wang H, Ng TB. Ganodermin, an antifungal protein from fruiting bodies of the medicinal mushroom Ganoderma lucidum. Peptides 27 (2006), pp. 27-30, 10.1016/j.peptides.2005.06.009.

30. Woo EE, Kim JY, Kim JS, Kwon SW, Lee IK, Yun BS. Mannonerolidol, a new nerolidol mannoside from culture broth of Schizophyllum commune. J Antibiot (Tokyo) 72 (2019), pp. 178-180, 10.1038/s41429-018-0130-3.

31. Yan Z, Chen X, Wu X, Li Q. Metabolic response of Pleurotus ostreatus to continuous heat stress. Front Microbiol. 2020;11:3148.

32. Zhang X, Hou X, Xu D, Xue M, Zhang J, Wang J, et al. Effects of carbon, nitrogen, ambient pH and light on mycelial growth, sporulation, sorbicillinoid biosynthesis and related gene expression in Ustilaginoidea virens. J Fungi 9 (2023), 390, 10.3390/jof9040390.

33. González MM, Pérez LM. Antibiosis de proteínas y metabolitos en especies de Trichoderma contra aislamientos paraguayos de Macrophomina phaseolina. Agrofor Américas 33 (2011), pp. 1-8.

34. Campuzano SE, Urquijo L, Valderrama J. Evaluación de la actividad celulolítica y quitinolítica de hongos filamentosos aislados de rizósfera de cultivos de papa para control de Rhizoctonia solani. Nova. 2017;15(28):45–55. doi:10.22490/24629448.2078.

35. Castañeda Alvarez E, Sánchez LC. Evaluación del crecimiento de cuatro especies del género Bacillus sp., primer paso para entender su efecto biocontrolador sobre Fusarium sp. Nova. 2016;14(26):53–62. doi:10.22490/24629448.1751.

36. Ostos Ortíz OL, Rosas Arango SM, González Devia JL. Aplicaciones biotecnológicas de los microorganismos. Nova. 2019;17(31):129–163. doi:10.22490/24629448.3629.

37. Ostos Ortíz OL, Rosas Arango SM, González Devia JL. Aplicaciones biotecnológicas de los microorganismos. Nova. 2019;17(31):129–163. doi:10.22490/24629448.3629.

license

How to Cite

Pérez Holguína, J. P., Quitian, J. C., Puentes Santos , A., Aguilera Becerra, A. M., & Fonseca-Guerra, I. R. (2025). Effect of Culture Medium Composition on the Antagonistic Capacity of Commercial Basidiomycetes against Fusarium spp. and Alternaria alternata. NOVA Biomedical Sciences Journal, 24(46), 59-76. https://doi.org/10.22490/24629448.10559

Download Citation

Almétricas

Metrics

Metrics Loading ...

Declaración de privacidad.

Effect of Culture Medium Composition on the Antagonistic Capacity of Commercial Basidiomycetes against Fusarium spp. and Alternaria alternata

How to Cite