La biotecnología de Bacillus thuringiensis en la agricultura

Diana Daniela Portela-Dussán; Alejandro Chaparro-Giraldo; Silvio Alejandro López-Pazos

doi:10.22490/24629448.1031

Vol. 11 No. 20 (2013)

Published

2013-12-15

PDF (Spanish)

Metrics

Metrics Loading ...

Bacillus thuringiensis biotechnology in agriculture

DOI: https://doi.org/10.22490/24629448.1031

Section

Research Article (before OJS)

Diana Daniela Portela-Dussán Grupo de Ingeniería Genética de Plantas, Departamento de Biología, Universidad Nacional de Colombia.

Alejandro Chaparro-Giraldo Grupo de Ingeniería Genética de Plantas, Departamento de Biología, Universidad Nacional de Colombia.

Silvio Alejandro López-Pazos Grupo de Ingeniería Genética de Plantas, Departamento de Biología, Universidad Nacional de Colombia. Facultad de Ciencias de la Salud, Universidad Colegio Mayor de Cundinamarca, Bogotá D.C., Colombia

Bacillus thuringiensis (Bt) is a Gram-positive rod-shape bacterium that during its sporulation phase produces a parasporal inclusion formed by Cry proteins having biological activity against pest insects. Because of these proteins, Bt shows toxicity toward pest insect larvae of Lepidoptera, Co-leoptera and Diptera orders among others, besides of friendly with the environment, and for this reason Bt has a very common use for commercial products and transgenic plants with Cry protein basis in agriculture. In this review is described the most important Bt factors and their application as biotechnological tool in agrobusiness, considering Bt bioassays.

Bacillus thuringiensis, Cry protein, pest insect, biopesticide, transgenic crop.

Oerke E.-C., Dehne H.-W. Safeguarding production-losses in major crops and the role of crop protection. Crop Protection. 2004; 23: 275-285.

Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D.R., Dean, D.H. Bacillus thuringiensis and Its Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 1998; 62: 775-806.

Porcar M., Juárez-Pérez V. Aislamiento y establecimiento de una colección de Bacillus thuringiensis. En Bacillus thuringiensis en el control biológico. Bravo, A. y Cerón, J. eds. Universidad Nacional de Colombia. Bogotá, Colombia. 2004; pp. 69-100.

Balaraman K. 2005. Occurrence and diversity of mosquitocidal strains of Bacillus thuringiensis. J. Vector Borne Dis. 42:81-86.

Iriarte J., Bel Y., Ferrandis M. D., Andrew, R., Murillo J. Ferre J. Caballero P. Environmental distribution and diversity of Bacillus thuringiensis in Spain. Syst. Appl. Microbiol. 998; 21: 97-106.

Ruiz de Escudero I., Ibañez I., Padilla M., Carnero A., Caballero P. Aislamiento y caracterización de nuevas cepas de Bacillus thuringiensis procedentes de tierras canarias. Bol. San. Veg. Plagas. 2004; 30: 703-712.

López-Pazos S.A., Martínez J.W., Castillo A.X., Cerón Salamanca J.A. Presence and significance of Bacillus thuringiensis Cry proteins associated with the Andean weevil Premnotrypes vorax (Coleoptera: Curculionidae). Rev. Biol. Trop. 2009; 57: 1235-1243.

Bravo A. Sarabia S., López, L., Ontiveros H., Abarca C., Ortiz A., Ortiz M., Lina L., Villalobos F. J., Pena G., Núñez-Valdez M. E., Soberon M., Quintero R. Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection. Appl. Environ. Microbiol. 1998; 64: 4965-4972.

Maduell P., Callejas R., Cabrera K.R., Armengol G.,Orduz S. Distribution and characterization of Bacillus thuringiensis on the phylloplane of species of Piper (Piperaceae) in three altitudinal levels. Microb. Ecol. 2002; 44: 144–153.

Vachon V., Laprade R., Schwartz J.L. año 2012. Current models of the mode of action of Bacillus thuringiensis insecticidal crystal proteins: A critical review. J. Invertebr. Pathol. In press.

van Frankenhuyzen K. 2009. Insecticidal activity of Bacillus thuringiensis crystal proteins. J Invertebr Pathol. 101: 1-16.

Galitsky N., Cody V., Wojtczak A., Ghosh D., Luft J.R. Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis. Acta. Crystallogr. 2001; D 57:1101–1109.

Morse R.J, Yamamoto T., Stroud R.M. Structure of Cry2Aa suggests an unexpected receptor binding epitope. Structure. 2001; 9: 409–417.

Pardo-López L., Soberón M., Bravo A. Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect resistance and consequences for crop protection. FEMS Microbiol. Rev. In press. 2012.

de Maagd R.A., Bravo A., Crickmore N. How Bacillus thuringiensis has evolved specific toxins to colonize the insect world. Trends Genet. 2001; 17: 193-199.

Sanahuja, G., Banakar, R., Twyman, R. M., Capell, T. and Christou, P. 2011. Bacillus thuringiensis: a century of research, development and commercial applications. Plant Biotechnology Journal, 9: 283 -300.

Aronson A.I., Shai Y. Why Bacillus thuringiensis insecticidal toxins are so effective: unique features of their mode of action. FEMS Microbiology Letters. 2001; 195: 1-8.

Bravo A., Gill S.S., Soberón M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007; 49: 423-435.

Tiewsiri K., Angsuthanasombat C. Structurally conserved aromaticity of Tyr249 and Phe264 in helix 7 is important for toxicity of the Bacillus thuringiensis Cry4Ba toxin. J Biochem Mol Biol. 2007; 40:163-171.

Roh J.Y., Choi J.Y., Li M.S., Jin B.R., Je Y.H. Bacillus thuringiensis as a Specific, Safe, and Effective Tool for Insect Pest Control. J. Microbiol. Biotechnol. 2007; 17: 547–559.

Pigott C.R., Ellar D.J. Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity. Microbiol. Mol. Biol. Rev. 2007;71: 255–281.

Bravo A, Gómez I, Porta H, García-Gómez BI, Rodriguez-Almazan C, Pardo L, Soberón M. 2013. Evolution of Bacillus thuringiensis Cry toxins insecticidal activity. Microb Biotechnol. 6: 17-26.

Shu C., Zhang J. año 2009. Current patents related to Bacillus thuringiensis insecticidal crystal proteins. Recent. Pat. DNA Gene Seq. 3: 26-28.

Rosas-García N.M. Biopesticide production from Bacillus thuringiensis: an environmentally friendly alternative. Recent Pat. Biotechnol. 2009; 3: 28-36.

Buitrago G. La producción de ingredientes activos con Bacillus thuringiensis. En Bacillus thuringiensis en el control biológico. Bravo, A. y Cerón, J. eds. Editorial Buena Semilla. Bogotá, Colombia. 2004; pp. 233-273.

Içgen Y., Içgen B., Özcengiz, G. año 2002 (a). Regulatión of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources. Rev. Microbiol. 153: 605-609.

Sauka D., Benintende G. Bacillus thuringiensis: generalidades un acercamiento a su empleo en el biocontrol de insectos lepidópteros que son plagas agrícolas. Revista Argentina de Microbiología. 2008; 40:124-140.

Brookes G., Barfoot P. GM Crops: The First Ten Years-Global Socio-Economic and Environmental Impacts. año 2006; ISAAA Brief No. 36. ISAAA: Ithaca, N..Y.

Kumar S, Chandra A., Pandey K.C. Bacillus thuringiensis (Bt) transgenic crop: an environment friendly insect-pest management strategy. J. Environ. Biol. 2008. 29:641-653.

Cerón J. Productos comerciales: nativos y recombinantes. En Bacillus thuringiensis en el control biológico. Bravo, A. y Cerón, J. eds. Editorial Buena Semilla. Bogotá, Colombia. año 2004; pp. 123-147.

Mohan Babu R., A. Sajeena, K. Seetharaman, Reddy M.S. año 2003. Advances in genetically engineered (transgenic) plants in pest management-an over view. Crop Protection. 22: 1071–1086.

Martínez, W. 2004. Evaluación de la toxicidad de Bacillus thuringiensis. En Bacillus thuringiensis en el control biológico. Bravo, A. y Cerón, J. eds. Universidad Nacional

de Colombia. Bogotá, Colombia. pp. 207-232. Navon, A. 2000. Bioassays of Bacillus thuringiensis products used against agricultural pests. CAB International. Bioassays of entomopathogenic microbes and ne matodes (eds. A. Navon and K.R.S. Ascher). pp. 1-24. Ochoa-Campuzano C, Real MD, Martínez-Ramírez AC, Bravo A, Rausell C. 2007. An ADAM metalloprotease is a Cry3Aa Bacillus thuringiensis toxin receptor. Biochem. Biophys. Res.

Commun. 362: 437-442.

Portela-Dussán, D. D., Chaparro-Giraldo, A., & López-Pazos, S. A. (2013). Bacillus thuringiensis biotechnology in agriculture. NOVA Biomedical Sciences Journal, 11(20), 87-96. https://doi.org/10.22490/24629448.1031

Download Citation

Published

Bacillus thuringiensis biotechnology in agriculture

Declaración de privacidad.

Keywords