Efecto del Mercurius-Heel®s sobre la citotoxicidad de fibroblastos gingivales humanos en un modelo in vitro

Gabriela Rueda; Dabeiba Adriana García; Liliana Catherine Patiño; Angélica Cagueñas; Mariana García

doi:10.22490/24629448.1027

Vol. 11 No. 20 (2013)

Published 2013-12-15

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Research Article (before OJS)

Effect of Mercurius-Heel®s on the cytotoxicity of human gingival fibroblasts in an in vitro model

DOI: https://doi.org/10.22490/24629448.1027

Gabriela Rueda Pontificia Universidad Javeriana. Centro de Investigaciones Odontológicas

Dabeiba Adriana García Pontificia Universidad Javeriana. Centro de Investigaciones Odontológicas

Liliana Catherine Patiño Pontificia Universidad Javeriana. Centro de Investigaciones Odontológicas

Angélica Cagueñas Pontificia Universidad Javeriana. Centro de Investigaciones Odontológicas

Mariana García Pontificia Universidad Javeriana. Centro de Investigaciones Odontológicas

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Abstract
References

Periodontal disease is pathology of infectious origin, characterized because it causes destructive consequences to the supporting tissue of the tooth, its treatment is aimed at the destruction of the etiologic agents and to the periodontal regeneration. An alternative is the use of homeo-pathic agents because they are natural and are managed to very low concentrations, one of them is the Mercurius Heel ® S as an adjunctive therapy in infectious diseases. In this work, we present the results of an investigation whose objective was to evaluate the effect of Mercurius Heel® S on the viability of human gingival fibroblasts, which were undergoing treatment with Mercurius Heel® S for 15 minutes and two hours to concentrations from 300mg to 0.00006mg. After these times, the treatment was removed and the cells were maintained for 24, 48, and 72 more hours. It was followed by a colorimetric assay for cell viability and proliferation called MTS of Promega ®.

Human gingival fibroblasts treated with Mercurius Heel® S showed an increase in cell prolife-ration compared with the untreated cells. Low medication concentrations of 0.0001 mg and 0.00006 mg showed a greater proliferation showed greater statistically differences. Finally, the effect of Mercurius Heel® was maintained for the first 48 hours. Considering the above, the Mercurius Heel® did not provide any cytotoxic effect on human gingival fibroblasts; on the contrary, the cells proliferated, suggesting its usefulness as supplementary treatment for periodontal disease.

keywords: cytotoxicity, gingival fibroblasts, Mercurius Heel ®S, viability.

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Rueda, G., García, D. A., Patiño, L. C., Cagueñas, A., & García, M. (2013). Effect of Mercurius-Heel®s on the cytotoxicity of human gingival fibroblasts in an in vitro model. NOVA Biomedical Sciences Journal, 11(20), 15-22. https://doi.org/10.22490/24629448.1027

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Effect of Mercurius-Heel®s on the cytotoxicity of human gingival fibroblasts in an in vitro model

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