Detección de anticuerpo tipo IgG contra Borrelia burgdorferi  en bovinos de tres regiones de Colombia

Lucía Constanza  Corrales Ramírez; Blanyfer Jholany Rodríguez Peña; Denisse Daniela  Ríos Suarez

doi:10.22490/24629448.10147

Vol. 23 No. 45 (2025): 2.

Published 2025-07-01

license

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Research Article (before OJS)

Detection of IgG-type antibody against Borrelia burgdorferi in cattle from three regions of Colombia

DOI: https://doi.org/10.22490/24629448.10147

Lucía Constanza Corrales Ramírez Universidad Colegio Mayor de Cundinamarca

Blanyfer Jholany Rodríguez Peña Ese Sor Teresa Adele

Denisse Daniela Ríos Suarez Ese Sor Teresa Adele

PDF (Spanish)

Abstract
References

Objective. To identify the presence of Borrelia burgdorferi by the IIF technique in cattle from three regions of Colombia, classify the vector and confirm the presence of spiroche tes in FSP of cattle and ticks. Methods. For the study, blood samples were taken from the coccygeal vein of 58 bovines from the municipalities of Silvania Cundinamarca and the municipalities of Guavatá and San Vicente de Chucurí Santander, to carry out the immunological test in serum, as well as the search for spirochetes in FSP from bovine and tick hemolymph by Wright’s stain. Results. A high seropositivity was obtained in the animals studied, the vector by morphometric characteristics was classified in the genus Rhipicephalus microplus, of the Ixodidae family, the tick with the highest incidence in Colombia and through special colorations the presence of spirochetes was evidenced in the samples of bovine blood and tick hemolymph. Conclusion. The country does not have studies of this nature, which is worrying since it is known that this bacterium is involved in the production of spontaneous abortions in cattle and can be transmitted as a zoonosis by consumption of unpasteurized milk and by exposure to the sting of its vector

keywords: Lyme disease, Borrelia burgdorferi, disease vectors, zoonosis

1. Steere AC. Lyme disease. N Engl J Med 2001; 345: 115–125.

2. Escudero-Nieto R, A Guerrero-Espejo. Enfermedades producidas por Borrelia. Enferm Infecc Microbiol Clin. 2005; 23, 232-240.

3. Villamil E, Epidemiología de “Borrelia Burgdorferi S L” (Enfermedad De Lyme) En Un Ecosistema de Pinar de Montaña Supramediterráneo, Veterinaria FDE. Universidad Complutense de Madrid. 2002.

4. Corrales L, Ávila S, Estupiñán S. Bacteriología Teoría y Práctica. Primera edición, Bogotá: Editorial Universidad Colegio Mayor de Cundinamarca; 2013: 281-283.

5. Rodríguez González I. Actualización acerca de Borrelia burgdorferi sensu lato y enfermedad de Lyme. Revista Cubana de Medicina Tropical. 2013; 65, (2), pp.149-165.

6. Villanueva Caro María Pilar. Borrelia burgdorferi: Una revisión bibliográfica sobre el agente causal de la enfermedad de Lyme. Tesis de maestría. Valdivia–Chile. Universidad Austral de Chile. 2009; Disponible en: http://cybertesis.uach.cl/tesis/uach/2009/fvv718b/doc/fvv718b.pdf

7. García M, Skinner C, Salas J, Ocampo J. Enfermedad de Lyme: actualizaciones. Gac Med Mex. 2014;150(1):84-95.

8. Bart Jan Kullberg, Hedwig D Vrijmoeth, Freek van de Schoor, Joppe W Hovius Review BMJ. Lyme borreliosis: diagnosis and management. BMJ.2020:369

9. Strnad M, Hönig V, Růžek D, Grubhoffer L, Rego ROM. Europe-Wide Meta-Analysis of Borrelia burgdorferi Sensu Lato Prevalence in Questing Ixodes ricinus Ticks. Appl Environ Microbiol. 2017 Jul 17;83(15):e00609-17. doi: 10.1128/AEM.00609-17. PMID: 28550059; PMCID: PMC5514677.

10. K Holubar. Erythema chronicum migrans: Afzelius and Lipschütz. J. Am Acad Dermatol. 1985;13(4):663-5.

11. Orestes Herrera Lorenzo, José Infante Ferrer, Carlos Ramírez Reyes, Hugo Lavastida Hernández. Enfermedad de Lyme: historia, microbiología, epizootiología y epidemiología. Revista Cubana de Higiene y Epidemiología. 2011; 50(2): 231-244

12. Batlle Almodovar M del C, Meneses FOD. Borreliosis de Lyme: Acercamiento a una enfermedad infecciosa emergente. Rev. cubana Hig Epidemiol [Internet]. 1997; vol.35(2):54. Disponible en: Cuba.

13. Angela Zuluaga de Cadena, Fernando Botero, Walter León Herrera. Jaime Robledo, Alonso Cortés, María Cristina Lotero. Enfermedad de Lyme: Un caso comprobado en Colombia. Instituto de Ciencias de las Salud CES. 2000: 14; (2): 44-50.

14. Villagra Mauricio, Martínez M. José. Enfermedad de Lyme: a propósito de un caso clínico importado. Rev. chil. infectol. [Internet]. 2018 [citado 2022 Oct 03];35( 5 ): 606-611. Disponible en: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182018000500606&lng=es.

15. Lucia Corrales, E. Stiven Quijano, Evelyn Ramírez. Detección de anticuerpos tipo IgG contra Borrelia Burgdorferi, y factores asociados a la enfermedad de Lyme en población canina, de los municipios Honda-Tolima, La Mesa y Chía-Cundinamarca. NOVA. 2022; 20 (38): 11-35.

16. Stewart PE, Rosa PA. Physiologic and Genetic Factors Influencing the Zoonotic Cycle of Borrelia burgdorferi. Curr Top Microbiol Immunol. 2018;415:63-82. doi: 10.1007/82_2017_43. PMID: 28864829.

17. Stevenson B. The Lyme disease spirochete, Borrelia burgdorferi, as a model vector-borne pathogen: insights on regulation of gene and protein expression. Curr Opin Microbiol. 2023 Aug;74:102332. doi: 10.1016/j.mib.2023.102332. Epub 2023 Jun 4. PMID: 37279610; PMCID: PMC10524203.

18. Ferdows MS, Barbour AG. Megabase-sized linear DNA in the bacterium Borrelia burgdorferi, the Lyme disease agent. Proc Natl Acad Sci USA. 1989; 86:5969–5973. [PMC free article] [PubMed] [Google Scholar]

19. Hyde FW, Johnson RC. Characterization of a circular plasmid from Borrelia burgdorferi, etiologic agent of Lyme disease. J Clin Microbiol. 1988; 26: 2203–2205. [PMC free article] [PubMed] [Google Scholar]

20. Jauris-Heipke S, Fuchs R, Motz M, et al. Genetic heterogeneity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi. Med Microbiol Immunol. 1993; 182:37–50. [PubMed] [Google Scholar]

21. Livey I, Gibbs CP, Schuster R, et al. Evidence for lateral transfer and recombination in OspC variation in Lyme disease Borrelia. Mol Microbiol. 1995; 18:257–269. [PubMed] [Google Scholar]

22. Zhang JR, Hardham JM, Barbour AG, et al. Antigenic variation in Lyme disease borreliae by promiscuous recombination of VMP-like sequence cassettes. Cell. 1997; 89: 275–285. [PubMed] [Google Scholar]

23. Jing-Ren Zhang, John M Hardham, Alan G Barbour, Steven J Norris. «Antigenic Variation in Lyme Disease Borreliae by Promiscuous Recombination of VMP-like Sequence Cassettes». Cell. 1997; 96, Issue 3.

24. Hirschfeld M, Kirschning CJ, Schwandner R, et al. Cutting edge: inflammatory signaling by Borrelia burgdorferi lipoproteins is mediated by toll-like receptor 2. J Immunol. 1999; 163, (5):2382–2386. [PubMed] [Google Scholar]

25. Wooten RM, Ma Y, Yoder RA, et al. Toll-like receptor 2 is required for innate, but not acquired, host defense to Borrelia burgdorferi. J Immunol. 2002;168: 348– 355. [PubMed] [Google Scholar]

26. Wang G, Ma Y, Buyuk A, et al. Impaired host defense to infection and Toll-like receptor 2-independent killing of Borrelia burgdorferi clinical isolates in TLR2-deficient C3H/HeJ mice. FEMS Microbiol Lett. 2004; 231(2):219–225. [PubMed] [Google Scholar]

27. Burgess EC, Wachal MD, Cleven TD. Borrelia burgdorferi infection in dairy cows, rodents, and birds from four Wisconsin dairy farms. Veterinary Microbiology. [Internet]. 1993; 35 (1-2); 61-77. [Cited 27 Ago. 2022]. Available in: https://www.sciencedirect.com/science/article/pii/037811359390116O

28. Veronesi F, Laus F, Passamonti F, Tesei B, Fioretti DP, Genchi C. Occurrence of Borrelia lusitaniae infection in horses. Veterinary Microbiology. [Internet]. 2012; 160 (3-4); 535-538. [Cited 30 abril. 2022]. Available in: https://www.sciencedirect.com/science/article/pii/S0378113512003811

29. Zuquete, Coelho, Rosa, Vaz, Casammá, Padre, et al. Title: Tick (Acari: Ixodidae) infestations in cattle along Geba River basin in Guinea-Bissau. [Internet]. 2016. [Cited 3 nov. 2021]. Available in: https://www.sciencedirect.com/science/article/abs/pii/S1877959X16301893

30. Lane RS, Piesman J, Burgdorfer W. Lyme borreliosis: relation of its causative agent to its vectors and hosts in North America and Europe. Annu Rev Entomol. 1991; 36: 587–609. [PubMed] [Google Scholar]

31. Burgdorfer W, Lane RS, Barbour AG, et al. The western black-legged tick, Ixodes pacificus, a vector of Borrelia burgdorferi. Am J Trop Med Hyg. 1985; 34: 925–930. [PubMed] [Google Scholar]

32. Miranda Jorge, Mattar Salim, Perdomo Katya, Palencia Luis. Seroprevalencia de Borreliosis, o Enfermedad de Lyme, en una Población Rural Expuesta de Córdoba, Colombia. Rev. salud pública [Internet]. 2009 June [cited 2022 Jul 03] ; 11( 3 ): 480-489. Available from: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0124-00642009000300016&lng=en.

33. Fajardo M, Fajardo L. La enfermedad de Lyme. Acta médica colombiana. 1994;19(4):193-8.

34. Răileanu C, Silaghi C, Fingerle V, Margos G, Thiel C, Pfister K, Overzier E. Borrelia burgdorferi Sensu Lato in Questing and Engorged Ticks from Different Habitat Types in Southern Germany. Microorganisms. 2021 Jun 10; 9(6):1266

35. Polanco Echeverry DN, Ríos Osorio LA. Aspectos biológicos y ecológicos de las garrapatas duras. Corpoica Cienc y Tecnol Agropecu [Internet]. 2016;17(1):81. Disponible en: http://revista.corpoica.org.co/index.php/revista/article/view/463

36. TICK IDENTIFICATION KEY. http://www.animaldisease.org/TICK/TIK/tick-key/index.htm

37. Unidos E, Agropecuaria S. Rhipicephalus ( Boophilus ) microplus Recursos en Internet. Cent Foof Secur Public Heal [Internet]. 2007;1-3. Disponible en: http://www.cfsph.iastate.edu/Factsheets/es/boophilus_microplus-es.pdf

38. Navarro García, Amanda Estrella. Borreliosis de Lyme: estudio de posibles vectores ixódidos y evaluación de métodos de diagnóstico microbiológico. Tesis doctoral. Universitat de València. Departament de Microbiologia i Ecologia. 2005. 204 p. http://hdl.handle.net/10803/10103

39. Craft J, Fischer D, Shimamoto G, Steere A. Antigens of Borrelia burgdorferi recognized during Lyme disease. Appearance of a new immunoglobulin M response and expansion of the immunoglobulin G response late in the illness. Journal of Clinical Investigation. 1986; 78(4): 934-939.

40. Magnarelli, Bushmich, Fikrig. Una comparación de las pruebas serológicas para la detección de anticuerpos séricos contra los antígenos de Borrelia burgdorferi de células enteras y recombinantes en bovinos. Can Vet J [Internet]. 2004 [Consultado 17 de Julio de 2022]; 45: 667–674. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC546445/

41. Ishikawa M. Epidemiologia da Borreliose de Lyme em Bovinos na região Sudeste do Brasil e Padronização Do diagnóstico Sorológico. Universidade Federal Rural Do Rio. Tese Magister scientiae. 1996. Disponible en http://www.adivaldofonseca.vet.br/Teses%20e%20dissertacoes/Ishikawa%201996%20mestrado.pdf

42. Dennis Verhaegh, Leo A B Joosten, Marije Oosting. The role of host immune cells and Borrelia burgdorferi antigens in the etiology of Lyme disease. Eur Cytokine Netw. 2017 Jun 1;28(2):70-84. [Citado 25 jul. 2022]. Disponible en: https://pubmed.ncbi.nlm.nih.gov/28840838/

43. Portillo A, Santibáñez S, Oteo J. Enfermedad de Lyme. Enfermedades Infecciosas y Microbiología Clínica. 2014;32:37-42.

44. Xavier P. La Maladie de Lyme Chez les Bovins Enquete Sero-Epidemiologique Dans L’est de la France. La Faculte de Medecine de Creteil; 2004.

45. Elhelw, El-Enbaawy, Samir. Lyme borreliosis: A neglected zoonosis in Egypt. Acta tropica [Internet]. 2014 [Consultado 17 de Julio 2021]; 140: 188-192. Disponible en: https://pubmed.ncbi.nlm.nih.gov/25239124/

46. Xinjiang: changes in tick distribution and the isolation of Borrelia burgdorferi sensu stricto. Parasites & vectors. [Internet]. 2015; 8 (1); 1-9. [Cited 2 nov. 2021]. Available in: https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-015-1021-0

47. Niu Q, Guan G, Yang J, Fu Y, Xu Z , Li Y, et al. Detection and differentiation of Borrelia burgdorferi sensu lato in ticks collected from sheep and cattle in China. BMC.[Internet]. 2011; 7 (1); 17-25. [Cited 30 jul. 2022]. Available in: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108939/

48. Helble JD, McCarthy JE, Hu LT. Interactions between Borrelia burgdorferi and its hosts across the enzootic cycle. Parasite Immunol. 2021 May;43(5):e12816. doi: 10.1111/pim.12816. Epub 2021 Jan 11. PMID: 33368329; PMCID: PMC8058304.

license

How to Cite

Corrales Ramírez, L. C., Rodríguez Peña, B. J., & Ríos Suarez, D. D. (2025). Detection of IgG-type antibody against Borrelia burgdorferi in cattle from three regions of Colombia. NOVA Biomedical Sciences Journal, 23(45), 59-83. https://doi.org/10.22490/24629448.10147

Download Citation

Almétricas

Metrics

Metrics Loading ...

Declaración de privacidad.

Detection of IgG-type antibody against Borrelia burgdorferi in cattle from three regions of Colombia

How to Cite