Fagocitosis en neonatos: determinación de valores normales para la micro técnica de fagocitosis y muerte intracelular de Candida Albicans

Janeth Navarrete; Gladys Pinilla; Liliana Muñoz L; Ariel Ruiz A

doi:10.22490/24629448.1011

Vol. 10 No. 17 (2012)

Published 2012-06-15

license

Research Article (before OJS)

Phagocytosis in neonates: determination of normal values for the micro technique phagocytosis and intracellular death of Candida Albicans

DOI: https://doi.org/10.22490/24629448.1011

Janeth Navarrete Universidad Colegio Mayor de Cundinamarca

Gladys Pinilla Universidad Colegio Mayor de Cundinamarca

Liliana Muñoz L Universidad Colegio Mayor de Cundinamarca

Ariel Ruiz A Universidad Nacional de Colombia

PDF (Spanish)

Abstract
References

Protect the host against strange agents is the main function of the immune system. Thus the innate immunity is the first line of defensemainly through physical and chemical barriers, and the phagocytosis mediated by the polymorphonuclear neutrophils (PMN). The neonates are considered immunologically immature, which makes them more susceptible to pathogenic microorganism.

One of the variations that seem to increment the risk of bacterial infections is the abnormality of the phagocytic system, which can lead to a deficiency in the ability of the PMN to phagocytose and kill microorganisms. Therefore, the objective of this study is to determine the reference value of the micro-technique of phagocytosis and intracellular death of Candida albicans, is a simple and effective technique that can be used to evaluate the phagocytic function since asses the phagocytic index of each PMN and percentage of intracellular death of phagocytized Candida. We analyzed cord blood samples from fifty one healthy neonates.The index of phagocytosis found was 2.64 (SD 0.51) (1.71 to 3.77) and median of percentage of intracellular death was 20% with P25 (18%), P75 (23%), ( 13 to 38%). The reference value using the same technique for adults are: phagocytosis index 2.7 (2.03-3.92) and percentage of intracellular death 31.31% (21-39%).

keywords: phagocytosis, neonates, polymorph nuclear neutrophil and intracellular death

Silva M. Neutrophils and macrohpages work in concert as inducers and effectors and adaptative immunity against extracellular and intracellular microbial pathogens. J Leuko Biol. 2010;87:805-813

Miguel A. Hernández-Urzúa, Anabell Alvarado- Navarro. Interleucinas e inmunidad innata. Rev Biomed. 2001; Vol. 12 /No. 4.: 12:272-280.

Rojas O, Arce P. Fagocitosis: mecanismos y consecuencias. Primera parte. Bioquimia. 2003; 28: 19-28.

Navarrete J, Pinilla G, Muñoz L, Ruiz A, Cifuentes Y. Determinación de valores de referencia para la microtécnica de fagocitosis y muerte intracelular de Cándida álbicans en un grupo de población sana en Bogotá. NOVA. 2007; 7:1-7.

Rojas O, Arce P. Fagocitosis: mecanismos y consecuencias segunda parte. Bioquimia. 2004; 29:18-31.

Rojas O. Arce P. Fagocitosis: mecanismos y consecuencias tercera parte. Bioquimia.; 29:55-67.

Penagos M, Berrón R, García M, Zaragoza J. El sistema inmune del recién nacido. Alergia, Asma e Inmunología Pediátrica. 2003; 12:63-68.

Petrova A, Mehta R. Dysfunction of innate immunity and associated pathology in neonates. Indian J Pediatr. 2007;74:185-91.

Currie AJ, Curtis S, Strunk T, Riley K, Liyanage K, Prescott S, Doherty D, Simmer K, Richmond P, Burgner D. Preterm infants have deficient monocyte and lymphocyte cytokine responses to group B streptococcus. Infect Immun. 2011; 79:1588-96.

Raimondi F, Ferrara T, Maffucci R, Milite P, Del Buono D, Santoro P, Grimaldi LC. Neonatal sepsis: a difficult diagnostic challenge. Clin Biochem. 2011;44:463-4.

Cairo MS. Neonatal neutrophil host defense. Prospects for immunologic enhancement during neonatal sepsis. Am J Dis Child. 1989;143:40-6.

Destin K, Linden J, Laforce-Nesbitt S, Bliss J. Oxidative burst and phagocytosis of neonatal neutrophils confronting Candida albicans and Candida parapsilosis. Early Hum Dev. 2009;85:531-53.

Sasanakul W, Singalavanija S, Hathirat P, Mahaphan W, Chuansumrit A . Hemogram in normal newborn babies with special reference to platelet count Southeast Asian J Trop Med Public Health. 1993; Suppl 1:237-40.

license

How to Cite

Navarrete, J., Pinilla, G., Muñoz L, L., & Ruiz A, A. (2012). Phagocytosis in neonates: determination of normal values for the micro technique phagocytosis and intracellular death of Candida Albicans. NOVA Biomedical Sciences Journal, 10(17), 25-32. https://doi.org/10.22490/24629448.1011

Download Citation

Almétricas

Metrics

Metrics Loading ...

Declaración de privacidad.

Phagocytosis in neonates: determination of normal values for the micro technique phagocytosis and intracellular death of Candida Albicans

How to Cite