Publicado: 2016-12-15

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Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria

Sección
Artículo producto de Investigación

Autores/as

Laura Cristina Pachón Meza
Sandra Milena Barrera Ayala
Instituto Nacional de Salud – INS , Colombia

Describir el ensayo realizado para la estandarización de la técnica de diagnóstico para malaria, denominada amplificación isotérmica mediada por loop (LAMP). Métodos. Se extrajeron 48 muestras divididas en 22 muestras para P. falciparum, 20 de P. vivax y 6 negativas para las mismas especies. Resultados. Se observaron falsos positivos, amplificaciones específicas que evidencian la inespecificidad de la técnica. Se propone estudiar los amplicones obtenidos por medio de restricción de fragmentos y secuenciación para elucidar el origen del problema.

Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria

Autores/as

  • Laura Cristina Pachón Meza
  • Sandra Milena Barrera Ayala Instituto Nacional de Salud – INS

DOI:

https://doi.org/10.22490/24629448.1748

Palabras clave:

Malaria, ampliación, isotérmica mediada por loop (LAMP), especificidad LAMP, P. falciparum, P. Vivax.

Resumen

Describir el ensayo realizado para la estandarización de la técnica de diagnóstico para malaria, denominada amplificación isotérmica mediada por loop (LAMP). Métodos. Se extrajeron 48 muestras divididas en 22 muestras para P. falciparum, 20 de P. vivax y 6 negativas para las mismas especies. Resultados. Se observaron falsos positivos, amplificaciones específicas que evidencian la inespecificidad de la técnica. Se propone estudiar los amplicones obtenidos por medio de restricción de fragmentos y secuenciación para elucidar el origen del problema.

Biografía del autor/a

Laura Cristina Pachón Meza

Joven investigador

Sandra Milena Barrera Ayala, Instituto Nacional de Salud – INS

Profesional especializado. Grupo de Parasitología, Red Nacional de Laboratorios, Instituto Nacional de Salud – INS

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Publicado

2016-12-15

Cómo citar

Pachón Meza, L. C., & Barrera Ayala, S. M. (2016). Inespecificidad persistente en amplificación isotérmica mediada por loop para diagnóstico de malaria. Nova, 14(26), 29–33. https://doi.org/10.22490/24629448.1748

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Sección

Artículo producto de Investigación