Evaluation of the genetic stability of Leptospira reference strains maintained under two conservation methods

Oscar Gabriel  Beltrán; Ligia Denise  Torres Higuera; José Luis  Rodríguez Bautista; Rocio Esperanza  Patiño Burbano

doi:10.22490/24629448.6585

Vol. 20 Núm. 39 (2022)

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2022-12-21

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Evaluación de la estabilidad genética de las cepas de referencia de Leptospira mantenidas bajo dos métodos de conservación

DOI: https://doi.org/10.22490/24629448.6585

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Artículo producto de Investigación

Oscar Gabriel Beltrán Universidad Colegio Mayor de Cundinamarca

Ligia Denise Torres Higuera Universidad Colegio Mayor de Cundinamarca

José Luis Rodríguez Bautista Universidade Federal Rural do Rio de Janeiro

Rocio Esperanza Patiño Burbano Corporación colombiana de investigación agropecuaria-AGROSAVIA

Objetivo. Se evaluó la estabilidad genética de cepas de Leptospira spp., mantenidas bajo dos sistemas de conservación. Metodología. Se determinó el grado de conservación de los genes 16S rRNA y ompL1 de 10 serovares de referencia de la colección de Leptospira spp., pertenecientes al Sistema de Bancos de Germoplasma de la nación para la Alimentación y la Agricultura (SBGNAA). Resultados. Se corroboró que los genes evaluados estos no han sufrido cambios considerables, puesto que se evidenciaron similitudes superiores al 99,69% para 16S rRNA y de 99,02 % para ompL1, en los alineamientos pareados. Conclusión. Se comprobó la estabilidad genética y la pureza de las cepas de referencia de Leptospira spp., mantenidas en criopreservación en nitrógeno líquido a -196 °C y a temperatura ambiente durante ocho años aproximadamente.

proteína ompL1, secuenciación de ADN, Leptospira, ARN ribosómico

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Beltrán, O. G. ., Torres Higuera, L. D. ., Rodríguez Bautista, J. L. ., & Patiño Burbano, R. E. . (2022). Evaluación de la estabilidad genética de las cepas de referencia de Leptospira mantenidas bajo dos métodos de conservación. Nova, 20(39), 65-79. https://doi.org/10.22490/24629448.6585

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