Caracterización Molecular de Cepas Colombianas de . a Través del Rflp-is200

Elizabeth Vásquez; Salim Máttar; Néstor Mossos; Darío Mogollón; Raúl Alberto Poutou Piñales BQ MSC

doi:10.22490/24629448.17

Vol. 3 No. 3 (2005)

Published 2005-06-15

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Molecular Characterization of Colombian Stumps of . Through the Rflp-is200

DOI: https://doi.org/10.22490/24629448.17

Elizabeth Vásquez

Salim Máttar

Néstor Mossos Laboratorio de Diagnostico

Darío Mogollón Laboratorio de Diagnostico

Raúl Alberto Poutou Piñales BQ MSC Pontificia Universidad Javeriana, Bogotá, D.C.

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Abstract
References

Molecular techniques, used for the genomic DNA analysis, as IS200 RFLP has shown to be efficient to solve phylogenetic and epidemiologic relationship between Salmonella spp., strains, isolated from poultry and human. Present study had the aim to determine whether the IS200 sequence was present in the Salmonella spp., strains isolated from both origins, to establish a possible transmition chain between both. Phylogenetic analysis of Salmonella strains showed 13 IS200 profiles that exhibit between 1 and 11 copies of the insertion sequence, located in a range size between 13 and 1.7kb. This work allowed to conclude that IS200 is an efficient, sensible and specific molecular marker, useful to epidemiological studies because it capacity of discriminating between different origin strains and showed the existence of a relationship between this strains.

The results obtained in this work showed the necessity of implementing sanitary rules to minimize the propagation of Salmonella spp., in the poultry production.

keywords: RFLP, Salmonella spp., IS200, dendogram.

Coll P. Interés de las Técnicas de Biología Molecular en el Estudio de las Infecciones Nosocomiales. Microb Clín 1994; 12: 369-371.

Beuzón CR, Casadesús J. Conserved Structure of IS200 Elements in Salmonella. Nuc Ac Res 1997; 25: 1355-1361.

Casadesús J, Roth J. Absence of Insertions Among Spontaneous Mutants of Salmonella typhimurium. Mol Gen Genet 1989; 216: 210-216.

Stanley J, Baquar N, Burnens A. Molecular Subtyping Scheme for Salmonella panama. J Clin Microbiol 1995; 33 (5): 1206- 1211.

Stanley J, Saunders N. DNA Insertion Sequences and the Molecular Epidemiology of Salmonella and Mycobacterium. J Med Microbiol 1996; 45: 236-251.

Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic Susceptibility Testing by a Standardized Single Disk Method. Am J Clin Pathol 1966; 45: 493-496.

Sambrook J, Manniatis T, Fritsch EF. Molecular Cloning: A Laboratory Manual. 2nd ed, ed. T Manniatis, Sambrook, J. Vol. 3. 1989; New York: Cold Spring Harbor Laboratory Press. 459.

Ausbel FM, Brent R, Ringston RE, Moore DD, Seidman JG, Smith JA, Struhl R. Current Protocols in Molecular Biology. 1987; New York, USA: Greene Publishing Associates & Wiley Interscience.

Olsen JE, Angel O, Threlfall EJ, Bisgaard M. Genomic Relationships Between Selected Phage Types of Salmonella enterica subsp. enterica Serotype typhimurium Defined by Ribotyping, IS200 Typing and PFGE. Microbiol 1997; 143: 1471-1479.

Olsen JE, Skov MN, Threlfall EJ, Brown DJ. Clonal Lines of Salmonella enterica serotype enteritidis Documented by IS200-, ribo-, Pulsed-Field Gel Electrophoresis and RFLP Typing. J Med Microbiol 1994; 40: 15-22.

Jaccard P. étude Comparative de la Distribution Floral dans une Portion des Alpes et des Jura. Bull Soc Vaudoise Sci Nat 1901; 37: 547-555.

Poutou R, Máttar S. Amplificación al Azar del Polimorfismo del ADN (RAPD) de Cepas de E.coli O157:H7 Aisladas de Pacientes Pediátricos. Pediatría 2000; 35 (3): 259-263.

Poutou R, Máttar S, Del Portillo P, Visbal J, Bermúdez A. RAPD Fingerprinting of Enterohaemorrhagic Escherichia coli O157:H7 Isolates in Santafé de Bogotá, DC, Colombia. Med Sci Res 2000; 28: 29-32.

Poutou RA, Máttar S, Moncayo S, Moreno C. Determinación Molecular De La Multirresistencia En Cepas Aisladas De Infección Nosocomial Del Hospital Universitario San Ignacio De Santafé De Bogotá. MEDICAS UIS 1999; 13 (1): 12- 18.

Bögel K. Salmonelosis: An International Comparison. In Symposium .Salmonellose. of German Veterinary Association. 1992. Bonn, Germany.

Rodríguez DC, Tauxe RV, Row B. International Increase in Salmonella enteritidis: A New Pandemic? Epid Infect 1990; 105: 21-27.

Alterkruse SF, Tollefson LK, Bögel K. Strategies for Salmonella enteritidis in Five Countries. Food Control 1993; 4: 10-16.

Talaska T. A Salmonella Data Bank for Routine Surveillance and Research. Bull WHO 1994; 72: 69-72.

Oosterom J. Epidemiológical Studies and Proposed Preventive Measures in the Fight Against Human Salmonelosis. Int J Food Microbiol 1991; 12: 41-52.

Borrego J, Castro D, Jiménez M, Luque A, Martínez E, Rodríguez A, Picazo J. Comparison of Epidemiological Markers of Salmonella Strains Isolated from Different Sources in Spain. J Clin Microbiol 1992; 30: 3058-3064.

Callow BR. A New Phage Typing Scheme for Salmonella typhimurium. J Hyg 1959; 57: 346-349.

McDonough Pl, Timoney JF, Jacoson RH, Khakhria R. Clonal Groups of Salmonella typhimurium in New York State. J Clin Microbiol 1989; 27: 622-627.

Millemann Y, Lesage MC, Chaslus E, Lafont J. Value of Plasmid Profiling, Ribotyping, and Detection of IS200 for Tracing Avian Isolates of Salmonella typhimurium and S. enteritidis. J Clin Microbiol 1995; 33: 173-179.

Gonzáles MA, Alvarez JA, Mendoza MC. Salmonella entérica en el área Sanitaria de Avilés (Asturias). Aspectos Microbiológicos, Clínicos y Epidemiológicos. Enf Infec Microb 1990; 8 (3): 144-147.

Cohen ML, Fontaine RE. An Assesment of Patient Related Economic Cost in an Outbreak of Salmonelosis. N Eng J Med 1978; 299: 459-460.

Le Minor L, Leminar S, Grimont PAD. Rapport Quatriennal du Center National des Salmonella sur l.origine et la Repartition en Serotypes des Souches Isolees en France Continentale au cours des Annees 1980 a 1983. Rev Epidem Sanite Pub 1985; 33: 13-21.

Liebisch B, Schwarz S. Evaluation of Molecular Techniques for Epidemiological Typing of Salmonella enterica subsp. enterica serovar dublin. J Clin Microbiol 1996; 34: 641- 646.

Liebisch B, Schwarz S. Moleculat Typing of Salmonella enterica subsp. enterica Serovar enteritidis Isolates. J Med Microbiol 1996; 44: 52-59.

Baxter C. Control de la Transmisión Vertical de Salmonella. Avicultura Profesional 1996; 14: 10-12.

Hermans PWM, Saha SK, Van Leeuwen WJ, Verbrugh HA, Van Belkum A, Goessens WHF. Molecular Typing of Salmonella typhi Strains from Dhaka (Bangladesh) and Development of ADN Probes Identifying Plasmid-encoded Multidrug-resistant Isolates. J Clin Microbiol 1996; 34 (6): 1373-1379.

Brunner F, Margadant A, Peduzzi R, Piffaretti JC. The Plasmid Pattern as Epidemiological Tool for Salmonella typhimurium Epidemics Comparison with the Lysotype. Clin Infec Dis 1983; 148: 711.

Martinetti G, Alt W. Egg tRNA Restriction Patterns and Plasmid Analysis as a Tool for Typing Salmonella enteritidis. Res Microbiol 1990; 141: 1151-1162.

Vatopoulos AC, Maines E, Balis E, Threlfall J, Kanelopou M, Kalaputhaki V, Malamou H, Legakis MJ. Molecular Epidemiology of Ampicilin Resistant Clinical Isolates of Salmonella enteritidis. J Clin Microbiol 1994; 32: 1322- 1325.

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Vásquez, E., Máttar, S., Mossos, N., Mogollón, D., & Poutou Piñales BQ MSC, R. A. (2005). Molecular Characterization of Colombian Stumps of . Through the Rflp-is200. NOVA Biomedical Sciences Journal, 3(3), 37-45. https://doi.org/10.22490/24629448.17

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Molecular Characterization of Colombian Stumps of . Through the Rflp-is200

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