Desafío diagnóstico en pediatría: detección de múltiples virus respiratorios y neurotrópicos mediante RT-PCR

Erica Marcela Aranguren Reina; Lorena García Agudelo; Julio Cesar Saavedra Parra

doi:10.22490/26194759.12055

Vol. 10 No. 1 (2027): Vol.10.Núm.1.(2027).

Published 2026-11-15

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Case reports

Diagnostic challenge in pediatrics: detection of multiple respiratory and neurotropic viruses using RT-PCR

DOI: https://doi.org/10.22490/26194759.12055

Erica Marcela Aranguren Reina Departamento de Investigación, Hospital Regional de la Orinoquía, Yopal, Colombia

Lorena García Agudelo Departamento de Investigación, Hospital Regional de la Orinoquía, Yopal, Colombia

Julio Cesar Saavedra Parra Departamento de Investigación, Hospital Regional de la Orinoquía, Yopal, Colombia

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Abstract
References

Objective. To describe the case of a pediatric patient with febrile seizures and respiratory symptoms in whom influenza A H1N1-2009 and enterovirus were detected by RT-PCR. Methodology. Case report of a 3-year-old girl with a history of pneumonia 30 days earlier, admitted with a two-day history of fever (39 °C) and a generalized tonic-clonic seizure with loss of consciousness and spontaneous recovery. The clinical course and the laboratory and molecular tests performed during 14 days of hospitalization were reviewed. Results. She had fever spikes between days 3 and 10, thrombocytopenia and leukopenia; tests for COVID-19, acute dengue, malaria, Chagas disease, leptospirosis and brucellosis were negative. The RT-PCR respiratory panel detected influenza A H1N1-2009 and the cerebrospinal fluid meningitis panel detected enterovirus; the patient received oseltamivir and improved until discharge. Conclusion. Timely molecular diagnosis guided appropriate treatment. The dual detection must be interpreted together with clinical and epidemiological data to distinguish between coinfection and a sequential course.

keywords: Viruses, Infectious diseases, Pediatrics, Molecular biology, Virology, Epidemiology

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How to Cite

Aranguren Reina, E. M., García Agudelo, L., & Saavedra Parra, J. C. (2026). Diagnostic challenge in pediatrics: detection of multiple respiratory and neurotropic viruses using RT-PCR. Biociencias, 10(1), 104-109. https://doi.org/10.22490/26194759.12055

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