Determinación cualitativa de compuestos fenólicos biológicos mediante el reactivo de Millon

Roger Alberto Rabelo Florez; Valerin Paola Hincapie Tapia; Yurley Patricia Guerrero Rodríguez; Katerine Escorcia Díaz; Danna Lamboglia Rodríguez; Yibelis Yacelis Carrillo Almeira; Shirly Gutiérrez Beleño; Candy Margarita Alvarez Rodriguez; Katya Gicela García Pérez; Edhisson Navarro Guerrero; Milena Cuellar Meneses; Alexandra Rosmery Sanjuan Arias; Sofía Michel Lobo Pacheco; Laura Liceth Caamaño Ballesteros; Cristian David Rodriguez Leiva; Rosa Delis Torres Romero; Yulitza Yaileth Caldozo Rosado; Estefany Lorena Rosado Sánchez; Vanessa Martínez Díaz; Yamilitza Araujo Calero; Dana Vanessa Amaris Meza; Naren David Castañeda Lobo; Maria José Lora Bertel; Ingrith Gisela Sarabia Cardenas; Tatiana Marcela Mendoza Salas; Carolina Judith De La Cruz García; Vicky Consuelo Caycedo Rodriguez

doi:10.22490/26194759.12037

Vol. 10 No. 1 (2027): Vol.10.Núm.1.(2027).

Published 2026-11-15

license

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Short articles

Qualitative determination of biological phenolic compounds using Millon's reagent

DOI: https://doi.org/10.22490/26194759.12037

Roger Alberto Rabelo Florez National Open and Distance University (UNAD)

Valerin Paola Hincapie Tapia National Open and Distance University (UNAD)

Yurley Patricia Guerrero Rodríguez National Open and Distance University (UNAD)

Katerine Escorcia Díaz National Open and Distance University (UNAD)

Danna Lamboglia Rodríguez National Open and Distance University (UNAD)

Yibelis Yacelis Carrillo Almeira National Open and Distance University (UNAD)

Shirly Gutiérrez Beleño National Open and Distance University (UNAD)

Candy Margarita Alvarez Rodriguez National Open and Distance University (UNAD)

Katya Gicela García Pérez National Open and Distance University (UNAD)

Edhisson Navarro Guerrero National Open and Distance University (UNAD)

Milena Cuellar Meneses National Open and Distance University (UNAD)

Alexandra Rosmery Sanjuan Arias National Open and Distance University (UNAD)

Sofía Michel Lobo Pacheco National Open and Distance University (UNAD)

Laura Liceth Caamaño Ballesteros National Open and Distance University (UNAD)

Cristian David Rodriguez Leiva National Open and Distance University (UNAD)

Rosa Delis Torres Romero National Open and Distance University (UNAD)

Yulitza Yaileth Caldozo Rosado National Open and Distance University (UNAD)

Estefany Lorena Rosado Sánchez National Open and Distance University (UNAD)

Vanessa Martínez Díaz National Open and Distance University (UNAD)

Yamilitza Araujo Calero National Open and Distance University (UNAD)

Dana Vanessa Amaris Meza National Open and Distance University (UNAD)

Naren David Castañeda Lobo National Open and Distance University (UNAD)

Maria José Lora Bertel National Open and Distance University (UNAD)

Ingrith Gisela Sarabia Cardenas National Open and Distance University (UNAD)

Tatiana Marcela Mendoza Salas National Open and Distance University (UNAD)

Carolina Judith De La Cruz García National Open and Distance University (UNAD)

Vicky Consuelo Caycedo Rodriguez National Open and Distance University (UNAD)

PDF (Spanish)

Abstract
References

Introduction: Tyrosine is a non-essential aromatic amino acid that acts as a major constituent in protein synthesis. Objective: The purpose of this research was to identify the presence of tyrosine in different biological samples. Methodology: A qualitative, experimental approach was used to identify phenolic compounds in various biological samples by observing color changes resulting from specific chemical reactions. Results: Tyrosine was not successfully identified in the test tubes containing the reagents and biological samples. Discussion: Negative results (yellow color) were observed in test tubes expected to test positive (red or pink color). This occurred because the test tubes were not properly shaken after adding Millon's reagent. Conclusion: The laboratory objective was not achieved, as the qualitative Millon's test did not allow for the detection of tyrosine in biological simples.

keywords: Millon's test, Tyrosine, Protein, Aromatic rings

Afanador, J. A. L. (2023). Trabajo inteligente productivo. Sello Editorial UNAD. https://doi.org/10.22490/9789584975096 DOI: https://doi.org/10.22490/9789584975096

Bordas, J., Philpot, R., Elkins, M., Nasr, M. R., & Cao, Z. T. (2024). B-310 Freezing and thawing serum without mixing prior to analysis causes false low Thyroglobulin results. Clinical Chemistry, 70(Supplement_1). https://doi.org/10.1093/clinchem/hvae106.667 DOI: https://doi.org/10.1093/clinchem/hvae106.667

Dai, Y., Yi, S., Lu, Z., Pan, X.-L., Li, Y.-X., Chen, Y.-Y., Huang, F., & Li, C.-H. (2026). Mechanisms underlying the changes in interaction characteristics of beef sarcoplasmic, myofibrillar protein and their correlations induced by ovalbumin and ethanol addition. Food Chemistry: X, 36(103905), 103905. https://doi.org/10.1016/j.fochx.2026.103905 DOI: https://doi.org/10.1016/j.fochx.2026.103905

Dolganyuk, V., Sukhikh, S., Kalashnikova, O., Ivanova, S., Kashirskikh, E., Prosekov, A., Michaud, P., & Babich, O. (2023). Food proteins: Potential resources. Sustainability, 15(7), 5863. https://doi.org/10.3390/su15075863 DOI: https://doi.org/10.3390/su15075863

Dziki, D., Krajewska, A., & Findura, P. (2024). Particle size as an indicator of wheat flour quality: A review. Processes (Basel, Switzerland), 12(11), 2480. https://doi.org/10.3390/pr12112480 DOI: https://doi.org/10.3390/pr12112480

Goulding, D. A., Fox, P. F., & O’Mahony, J. A. (2020). Milk proteins: An overview. In Milk Proteins (pp. 21–98). Elsevier. https://doi.org/10.1016/b978-0-12-815251-5.00002-5 DOI: https://doi.org/10.1016/B978-0-12-815251-5.00002-5

Kulkarni, V. V., Girish, P. S., Barbuddhe, S. B., Naveena, B. M., & Muthukumar, M. (2021). Meat Protein Analysis. In Analytical Techniques in Meat Science (pp. 8–21). CRC Press. https://doi.org/10.1201/9781003230892-3 DOI: https://doi.org/10.1201/9781003230892-3

Li, Z., Huang, X., Tang, Q., Ma, M., Jin, Y., & Sheng, L. (2022). Functional properties and extraction techniques of chicken egg white proteins. Foods (Basel, Switzerland), 11(16), 2434. https://doi.org/10.3390/foods11162434 DOI: https://doi.org/10.3390/foods11162434

Małecki, J., Muszyński, S., & Sołowiej, B. G. (2021). Proteins in Food Systems—Bionanomaterials, Conventional and Unconventional Sources, Functional Properties, and Development Opportunities. Polymers, 13(15), 1–21. https://doi.org/10.3390/polym13152506 DOI: https://doi.org/10.3390/polym13152506

Mannott, I., Bosse, R., & Gibis, M. (2026). Recent Advances in Egg White Biotechnology, Microbiology, and Quality. Comprehensive Reviews in Food Science and Food Safety, 25(3), e70495. https://doi.org/10.1111/1541-4337.70495 DOI: https://doi.org/10.1111/1541-4337.70495

Mezzetti, M., Passamonti, M. M., Dall’Asta, M., Bertoni, G., Trevisi, E., & Ajmone Marsan, P. (2024). Emerging Parameters Justifying a Revised Quality Concept for Cow Milk. Foods (Basel, Switzerland), 13(11). https://doi.org/10.3390/foods13111650 DOI: https://doi.org/10.3390/foods13111650

Muñoz, Y. L. A., & Tueros, V. J. M. (2019). Efecto cicatrizante del gel elaborado con extracto etanólico de las flores de Matricaria Chamomilla l. (manzanilla) en ratones albinos [ Químico Farmacéutico y Bioquímico, Universidad INCA Garcilaso De La Vega]. https://hdl.handle.net/20.500.11818/4540

Pedley, E. (1950). A volumetric determination of barbituric acid derivatives. The Journal of Pharmacy and Pharmacology, 2(1), 39–44. https://doi.org/10.1111/j.2042-7158.1950.tb12901.x DOI: https://doi.org/10.1111/j.2042-7158.1950.tb12901.x

Ramya, S., Chandran, M., King, I. J., Jayakumararaj, R., Loganathan, T., Pandiarajan, G., Kaliraj, P., Grace Lydial Pushpalatha, G., Abraham, G. C., Vijaya, V., Aruna, D., Sutha, S., & Dhakar, R. C. (2022). Phytochemical Screening, GCMS and FTIR Profile of Bioactive Compounds in Solanum lycopersicum Wild Fruits collected from Palani Hill Ranges of the Western Ghats. Journal of Drug Delivery and Therapeutics, 12(6), 56–64. https://doi.org/10.22270/jddt.v12i6.5665 DOI: https://doi.org/10.22270/jddt.v12i6.5665

Rasch, E., & Swift, H. (1960). MICROPHOTOMETRIC ANALYSIS OF THE CYTOCHEMICAL MILLON REACTION. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/8.1.4 DOI: https://doi.org/10.1177/8.1.4

Remi, V.-O., & Esther, O. M. (2025). Characterization of phytochemical and nutritional composition of wheat flour (Triticum aestivum). GSC Advanced Research and Reviews, 22(1), 031–036. https://doi.org/10.30574/gscarr.2025.22.1.0001 DOI: https://doi.org/10.30574/gscarr.2025.22.1.0001

Samira, M., El-Mokhtar, D. A., Noureddine, M., Mustapha, D., Mohammed, Z., Mohammed, B., & Mohammed, B. (2021). Egg quality from avian species: Electrophoretic characterization of egg white proteins. Animal Production, 34–43. https://doi.org/10.20884/1.jap.2021.23.1.2 DOI: https://doi.org/10.20884/1.jap.2021.23.1.2

Shan, K., Zhou, G., & Li, C. (2025). Meat phenomics: A novel concern linking meat across the whole chain. Meat Science, 229(109913), 109913. https://doi.org/10.1016/j.meatsci.2025.109913 DOI: https://doi.org/10.1016/j.meatsci.2025.109913

Shi, K., Shi, Y., & Ji, A. (2026). Recent advances in metabolic engineering for microbial production of L-tyrosine. World Journal of Microbiology and Biotechnology, 42(1), 38. https://doi.org/10.1007/s11274-025-04776-y DOI: https://doi.org/10.1007/s11274-025-04776-y

Silvano, F., Santos, L. H., & Campos, C. E. (2025). Urinary tyrosine detection for cancer screening and metabolic disorder diagnosis: a comprehensive evaluation of a colorimetric method. https://doi.org/10.5281/ZENODO.17630234

Tamura Z., Iitaka Y., & Kido Y. (1966). Mechanism of the Millon reaction. 分析化学, 15(10), 1136–1137. https://doi.org/10.2116/bunsekikagaku.15.1136 DOI: https://doi.org/10.2116/bunsekikagaku.15.1136

Tomczak, A., Zielińska-Dawidziak, M., Klimowicz, P., Hejdysz, M., Kaczmarek, S., Siger, A., & Cieślak, A. (2024). Modification of the protein amino acid content in hen eggs as a consequence of different concentrations of lupine and soy in feed. Molecules (Basel, Switzerland), 29(16), 3727. https://doi.org/10.3390/molecules29163727 DOI: https://doi.org/10.3390/molecules29163727

Zou, Y., Chen, Y., Meng, Q., Wang, Y., & Zhang, Y. (2024). Cow milk fatty acid and protein composition in different breeds and regions in China. Molecules (Basel, Switzerland), 29(21), 5142. https://doi.org/10.3390/molecules29215142 DOI: https://doi.org/10.3390/molecules29215142

license

How to Cite

Rabelo Florez, R. A., Hincapie Tapia, V. P., Guerrero Rodríguez, Y. P., Escorcia Díaz, K., Lamboglia Rodríguez, D., Carrillo Almeira, Y. Y., Gutiérrez Beleño, S., Alvarez Rodriguez, C. M., García Pérez, K. G., Navarro Guerrero, E., Cuellar Meneses, M., Sanjuan Arias, A. R., Lobo Pacheco, S. M., Caamaño Ballesteros, L. L., Rodriguez Leiva, C. D., Torres Romero, R. D., Caldozo Rosado, Y. Y., Rosado Sánchez, E. L., Martínez Díaz, V., … Caycedo Rodriguez, V. C. (2026). Qualitative determination of biological phenolic compounds using Millon’s reagent. Biociencias, 10(1), 89-97. https://doi.org/10.22490/26194759.12037

Download Citation

Almétricas