Isolamento de lectinas por cromatografia de afinidade

José Camilo Torres Romero, Myriam Janeth Ortega Torres, Jhon Alexander Infante Betancour, Cicero Antonio Maia Cavalcante

Resumen


Cromatografia de afinidade é uma técnica usada para separar compostos, como, por exemplo, determinadas proteínas, que têm a capacidade de se ligar não covalentemente e reversivelmente a moléculas específicas conhecidas como ligantes. Esse método difere das técnicas de cromatografia clássica devido a proteína conseguir ser separada com base em uma única propriedade bioquímica. Em cromatografia de afinidade, o ligante está ligado covalentemente à matriz, que deve ser quimicamente inerte, porosa e também ter uma variedade de grupos funcionais adequados para acoplamento com ligantes diferentes.   Várias matrizes e ligantes são usados em cromatografia de afinidade, dependendo da proteína a ser purificada. Esse trabalho descreverá alguns aspectos importantes para o isolamento de lectinas através da técnica de cromatografia de afinidade utilizando-se carboidratos.

Palabras clave


proteínas; lectinas; carboidratos; isolectinas; cromatografia.

Referencias


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DOI: http://dx.doi.org/10.22490/21456453.1837

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